Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T00:36:06.106Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of polymorphic proteins of the human malaria parasite Plasmodium falciparum

Published online by Cambridge University Press:  14 April 2009

Brian Fenton  and
David Walliker
Brian Fenton*
Affiliation:
Department of Genetics, University of Edinburgh, West Mains Road, Edinburgh EH9 3JN, Scotland, U.K.
David Walliker
Affiliation:
Department of Genetics, University of Edinburgh, West Mains Road, Edinburgh EH9 3JN, Scotland, U.K.
*
* Corresponding author.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Five polymorphic proteins, detected by two-dimensional electrophoresis, were analysed in the parents and progeny of a cross between two clones of the malaria parasite Plasmodium falciparum. The information obtained showed that different forms of each protein were determined by allelic variants of each respective gene. One protein was identified as the parasite enzyme adenosine deaminase. Recombinant parasites were produced at a higher than expected frequency.

Type
Research Article
Information
Genetics Research , Volume 55 , Issue 2 , April 1990 , pp. 81 - 86
Copyright
Copyright © Cambridge University Press 1990

References

Dadonna, P. E., Weismann, W. P., Lambros, C., Kelley, W. N. & Webster, H. K. (1984). Human malaria parasite adenosine deaminase. Journal of Biological Chemistry 259, 1472–1475.Google Scholar
Fenton, B. (1987). Peptide digest studies of polymorphic proteins of Plasmodium falciparum. Molecular and Biochemical Parasitology 24, 13–22.Google Scholar
Fenton, B., Walker, A. & Walliker, D. (1985). Protein variation in clones of Plasmodium falciparum detected by two dimensional electrophoresis. Molecular and Biochemical Parasitology 16, 173–183.Google Scholar
Haynes, J. D., Diggs, C. L., Hines, F. A. & Desjardins, R. E. (1976). Culture of the human malaria parasite Plasmodium falciparum. Nature 263, 767–769.CrossRefGoogle Scholar
Kemp, D. J., Thompson, J. K., Walliker, D. & Corcoran, L. M. (1987). Molecular karyotype of Plasmodium falciparum: conserved linkage groups and expendable histidine rich protein genes. Proceedings of the National Academy of Sciences, U.S.A. 84, 7672–7676.CrossRefGoogle ScholarPubMed
O'Farrell, P. H. (1975). High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–4021.CrossRefGoogle ScholarPubMed
Prensier, G. & Slomianny, C. (1986). The karyotype of Plasmodium falciparum determined by ultrastructural serial sectioning and 3D reconstruction. Journal of Parasitology 72, 731–736.Google Scholar
Rosario, V. E. (1981). Cloning of naturally occurring mixed infections of malaria parasites. Science 212, 1037–1038.CrossRefGoogle ScholarPubMed
Sanderson, A., Walliker, D. & Molez, J.-F. (1981). Enzyme typing of Plasmodium falciparum from some African and other old world countries. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 263–267.Google Scholar
Sinnis, P. & Wellems, T. E. (1988). Long range restriction maps of Plasmodium falciparum chromosomes: crossing- over and size variation among homologous chromosomes from geographically distant isolates. Genomics 3, 287–295.CrossRefGoogle ScholarPubMed
Thaithong, S., Beale, G. H., Fenton, B., McBride, J., Rosario, V., Walker, A. & Walliker, D. (1984). Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum. Transactions of the Royal Society of Tropical Medicine and Hygiene 78, 242–245.CrossRefGoogle Scholar
Trager, W. & Jensen, J. B. (1976). Human malaria parasites in continuous culture. Science 193, 673–675.CrossRefGoogle ScholarPubMed
Walliker, D., Carter, R. & Morgan, S. (1973). Genetic recombination in Plasmodium berghei. Parasitology 66, 309–320.Google Scholar
Walliker, D., Carter, R. & Sanderson, A. (1975). Genetic studies on Plasmodium chabaudi: recombination between enzyme markers. Parasitology 70, 19–24.Google Scholar
Walliker, D., Quakyi, I. A., Wellems, T. E., McCutchan, T. F., Szarfman, A., London, W. T., Corcoran, L. M., Burkot, T. R. & Carter, R. (1987). Genetic analysis of the human malaria parasite Plasmodium falciparum. Science 236, 1661–1666.CrossRefGoogle ScholarPubMed
Wellems, T. E., Walliker, D., Smith, C. L., Rosario, V. E., Maloy, W. L., Howard, R. J., Carter, R. & McCutchan, T. F. (1987). A histidine rich protein gene marks a linkage group favored strongly in a genetic cross of Plasmodium falciparum. Cell 49, 633–642.Google Scholar
World Health Organization (1985). Tropical Disease Research, Seventh Programme Report. World Health Organisation, Geneva.Google Scholar