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Genetic analysis of axonemal mutants in Paramecium tetraurelia defective in their response to calcium

Published online by Cambridge University Press:  14 April 2009

Robert D. Hinrichsen  and
Ching Kung
Robert D. Hinrichsen
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706
Ching Kung
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706 Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706
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Six axonemal mutants of Paramecium tetraurelia have been isolated that are unable to respond properly to calcium. The mutants, designated atalantas, cannot swim backward when stimulated by ions or heat. Genetic analyses reveal that all six mutants are recessive and fall into four complementation groups. Three of the mutants in one complementation group are phenotypically non-leaky, one is leaky and two are extremely leaky, only displaying their phenotypes at elevated temperatures. The complete mutants, ataA, are also abnormal in their forward swimming. This abnormality co-segregates with the inability to swim backward. ataA1 is not allelic to several membrane mutants of P. tetraurelia.

Type
Research Article
Information
Genetics Research , Volume 43 , Issue 1 , February 1984 , pp. 11 - 20
Copyright
Copyright © Cambridge University Press 1984

References

REFERENCES

Bessen, M., Fay, R. & Witman, G. (1980). Calcium control of waveform in isolated flagellar axonemes of Chlamydomonas. Journal of Cell Biology 86, 446–455.CrossRefGoogle ScholarPubMed
Gibbons, B. H. & Gibbons, I. R. (1980). Calcium-induced quiescence in reactivated sea urchin sperm. Journal of Cell Biology 84, 13–27.Google Scholar
Gibbons, I. R. (1981). Cilia and flagella of eukaryotes. Journal of Cell Biology 91, 197s–224s.Google Scholar
Hayashi, M. & Takahashi, M. (1979). Ciliary adenosinetriphosphatase from a slow swimming mutant of Paramecium caudatum. Journal of Biological Chemistry 254, 11561–11565.Google Scholar
Hennessey, T. & Nelson, D. (1979). Thermosensory behaviour in Paramecium tetraurelia. Journal of General Microbiology 112, 337–347.Google Scholar
Hinrichsen, R. D., Saimi, Y., Hennessey, T. & Kung, C. (1983). Mutants in Paramecium tetraurelia defective in their axonemal response to calcium. Submitted for publication to Journal of Cell Biology.Google Scholar
Holwill, M. E. J. & McGregor, J. L. (1975). Control of flagellar wave movement in Crithidia oncopelti. Nature 255, 156–158.Google Scholar
Huang, B., Pipero, G., Ramanis, Z. & Luck, D. J. L. (1981). Radial spokes of Chlamydomonas flagella: genetic analysis and assembly and function. Journal of Cell Biology 88, 80–88.CrossRefGoogle ScholarPubMed
Kung, C. (1971). Genic mutations with altered systems of excitation in Paramecium aurelia. II. Mutagenesis, screening and genetic analysis of mutants. Genetics 69, 29–45.Google Scholar
Kung, C. (1979). Biology and genetics of Paramecium behavior. In Neurogenetics: Genetic Approaches to the Nervous System (ed. Breakfield, X. O.), pp. 1–26. New York: Elsevier.Google Scholar
Kung, C., Chang, Y.Satow, Y.Van Houten, J. & Hansma, H. (1975). Genetic dissection of behavior in Paramecium. Science (Wash.) 188, 898–904.Google Scholar
Kung, C. & Naitoh, Y. (1973). Calcium-induced ciliary reversal in the extracted models of ‘Pawns’, a behavioral mutant of Paramecium. Science (Wash.) 179, 195–196.Google Scholar
Kung, C. & Saimi, Y. (1982). The physiological basis of taxis in Paramecium. Annual Review of Physiology 44, 519–534.CrossRefGoogle ScholarPubMed
Naitoh, Y. & Eckert, R. (1974). The control of ciliary activity of protozoa. In Cilia and Flagella, ed. Sleigh, M., pp. 305–352. New York: Academic Press.Google Scholar
Naitoh, Y. & Kaneko, H. (1972). Reactivated triton-extracted models of Paramecium. Modification of ciliary movement by calcium ions. Science (Wash.) 176, 523–524.Google Scholar
Nakamura, S. (1979). A backward swimming mutant of Chlamydomonas reinhardii. Experimental Cell Research 123; 441–444.CrossRefGoogle ScholarPubMed
Satir, P. (1975). Ionophore-mediated calcium induces mussel gill ciliary arrest. Science (Wash.) 190, 586–588.Google Scholar
Schmidt, J. A. & Eckert, R. (1976). Calcium couples flagellar reversal to photo-stimulation in Chlamydomonas reinhardii. Nature (Lond.) 262, 713–715.CrossRefGoogle Scholar
Sonneborn, T. M. (1970). Methods in Paramecium research. In Methods in Cell Physiology, vol. 4 (ed. Prescott, D. M.), pp. 241–331. New York: Academic Press.Google Scholar
Sturgess, J. M., Chao, J., Wong, J., Aspin, N. & Turner, J. A. P. (1979). Cilia with defective radial spokes: a cause of human respiratory disease. New England Journal of Medicine 300, 53–56.CrossRefGoogle Scholar
Takahashi, M. & Naitoh, Y. (1978). Behavioral mutants of Paramecium caudatum with defective membrane electrogenesis. Nature (Lond.) 271, 616–619.CrossRefGoogle Scholar
Witman, G. B., Plummer, J. & Sander, G. (1978). Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition and function of specific axonemal components. Journal of Cell Biology 76, 729–747.Google Scholar