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Genetic analysis of axonemal mutants in Paramecium tetraurelia defective in their response to calcium

Published online by Cambridge University Press:  14 April 2009

Robert D. Hinrichsen  and
Ching Kung
Robert D. Hinrichsen
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706
Ching Kung
Affiliation:
Laboratory of Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706 Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706

Summary

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Six axonemal mutants of Paramecium tetraurelia have been isolated that are unable to respond properly to calcium. The mutants, designated atalantas, cannot swim backward when stimulated by ions or heat. Genetic analyses reveal that all six mutants are recessive and fall into four complementation groups. Three of the mutants in one complementation group are phenotypically non-leaky, one is leaky and two are extremely leaky, only displaying their phenotypes at elevated temperatures. The complete mutants, ataA, are also abnormal in their forward swimming. This abnormality co-segregates with the inability to swim backward. ataA1 is not allelic to several membrane mutants of P. tetraurelia.

Type
Research Article
Information
Genetics Research , Volume 43 , Issue 1 , February 1984 , pp. 11 - 20
Copyright
Copyright © Cambridge University Press 1984

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