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Genetic analysis of amidase mutants of Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

Joan L. Betz ,
Jane E. Brown ,
Patricia H. Clarke  and
Martin Day
Joan L. Betz
Affiliation:
Department of Biochemistry, University College London, Gower Street, London WC1E 6BT
Jane E. Brown
Affiliation:
Department of Biochemistry, University College London, Gower Street, London WC1E 6BT
Patricia H. Clarke
Affiliation:
Department of Biochemistry, University College London, Gower Street, London WC1E 6BT
Martin Day
Affiliation:
Department of Biochemistry, University College London, Gower Street, London WC1E 6BT
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Mutants of Pseudomonas aeruginosa, which differed in amide growth phenotype from the wild-type strain, were subjected to genetic analysis using the generalized transducing phage F116. The map order of some mutational sites was determined by 3-factor crosses in which a mutation in the linked regulator gene amiR was used as the outside marker to determine the relative order of mutations in the amidase structural gene amiE. Acetamide-positive transductants were recovered in crosses between amidase-negative strains and strains PhB3(PAC377), V2(PAC353) and V5(PAC356) producing mutant amidases which hydrolyse phenylacetamide and valeramide but not acetamide. Some recombinants carried the mutation amiE16 determining the properties of the mutant B amidase produced by strain B6(PAC351) from which both PhB and V class mutants were derived, while other recombinants produced A amidase determined by the wild-type amiE gene.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 3 , June 1974 , pp. 335 - 359
Copyright
Copyright © Cambridge University Press 1974

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