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Genetic analysis of actidione-resistant mutants in the Myxomycete Physarum polycephalum, Schw.

Published online by Cambridge University Press:  14 April 2009

Jennifer Dee
Affiliation:
Department of Zoology, University College London, W.C.I
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1. Improved methods for the culture and preservation of Physarum polycephalum amoebae have been developed.

2. Strains of amoebae have been isolated which are stably resistant to 4, 8 or 16 μg./ml. actidione.

3. Sensitivity vs. resistance to 4 μg./ml. actidione is probably controlled by a single pair of alleles at a locus unlinked to the mating-type locus.

4. Plasmodia homozygous or heterozygous for the allele determining resistance at this locus are no more resistant to actidione than plasmodia homozygous for the allele determining sensitivity.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1966

References

Bennett, L., Smithers, D. & Ward, C. T. (1964). Inhibition of DNA synthesis in mammalian cells by actidione. Biochim. biophys. Acta, 87, 6069.Google ScholarPubMed
Cooney, W. J. & Bradley, S. G. (1962). Action of cycloheximide on animal cells. In Antimicrobial Agents and Chemotherapy — 1961 (Finland, M. & Savage, G. M., eds.), pp. 237244. Ann Arbor: Braun-Brumfield.Google Scholar
Cummins, J. E., Brewer, E. N. & Rusch, H. P. (1965). The effect of actidione on mitosis in the slime mould Physarum polycephalum. J. Cell Biol. 27, 337341.CrossRefGoogle Scholar
Daniel, J. W. & Baldwin, H. H. (1964). Methods of culture for plasmodial Myxomycetes. Methods in Cell Physiology (Prescott, D. M., ed.), vol. 1, pp. 941. New York: Academic Press.Google Scholar
Daniel, J. W. & Rusch, H. P. (1962). Method for inducing sporulation of pure cultures of the Myxomycete Physarum polycephalum. J. Bact. 83, 234240.CrossRefGoogle ScholarPubMed
Dee, J. (1960). A mating-type system in an acellular slime-mould. Nature, Lond. 185, 780781.CrossRefGoogle Scholar
Dee, J. (1962). Recombination in a Myxomycete, Physarum polycephalum Schw. Genet. Res. 3, 1123.CrossRefGoogle Scholar
Grover, R. K. & Moore, J. D. (1961). Adaptation of Sclerotinia fructicola and Sclerotinia laxa to higher concentrations of fungicides. Phytopathology, 51, 399401.Google Scholar
Gundersen, K. & Wadstein, T. (1962). Morphological changes and resistance induced in Saccharomyces pastorianus by the antibiotic cycloheximide. J. gen. Microbiol. 28, 215230.CrossRefGoogle ScholarPubMed
Guttes, E. & Guttes, S. (1964). Mitotic synchrony in the plasmodia of Physarum polycephalum and mitotic synchronization by coalescence of micro-plasmodia. Methods in Cell Physiology (Prescott, D. M., ed.), vol. 1, pp. 4354. New York: Academic Press.Google Scholar
Hsu, K. S. (1963). The genetic basis of actidione resistance in Neurospora. J. gen. Microbiol. 32, 341347.CrossRefGoogle ScholarPubMed
Kerr, N. S. (1959). Actidione-resistant mutants of Didymium nigripes. J. Protozool. 6, (Suppl.), 16.Google Scholar
Kerr, N. S. (1965). Disappearance of a genetic marker from a cytoplasmic hybrid plasmodium of a true slime mould. Science, N.Y. 147, 15861588.CrossRefGoogle Scholar
Kerridge, D. (1958). The effect of actidione and other antifungal agents on nucleic acid and protein synthesis in Saccharomyces carlsbergensis. J. gen. Microbiol. 19, 497506.CrossRefGoogle ScholarPubMed
De Kloet, S. R. (1965). Accumulation of RNA with a DNA-like base composition in Saccharomyces carlsbergensis in the presence of cycloheximide. Biochem. biophys. Res. Commun. 19, 582586.CrossRefGoogle ScholarPubMed
Lee, B. K. & Wilkie, D. (1965). Sensitivity and resistance of yeast strains to actidione and actidione derivatives. Nature, Lond. 206, 9092.CrossRefGoogle ScholarPubMed
Loefer, J. B. & Matney, T. S. (1952). Growth inhibition of free living Protozoa by actidione. Physiol. Zool. 25, 272276.CrossRefGoogle Scholar
Middlekauff, J. E., Hino, S., Yang, S. P., Lindgeren, C. C. & Lindegren, G. (1957). Gene control of resistance vs. sensitivity to actidione in Saccharomyces. Genetics, 42, 6671.CrossRefGoogle ScholarPubMed
Siegel, M. & Sisler, H. (1964). Site of action of cycloheximide in cells of Saccharomyces pastorianus. Biochim. biophys. Acta, 87, 7089.Google ScholarPubMed
Warr, J. R. & Roper, J. A. (1965). Resistance to various inhibitors in Aspergillus nidulans. J. gen. Microbiol. 40, 273281.CrossRefGoogle ScholarPubMed
Whiffen, A. J., Bohonos, N. & Emerson, R. L. (1946). The production of an antifungal antibiotic by Streptomyces griseus. J. Bact. 52, 610611.CrossRefGoogle ScholarPubMed
Wilkie, D. & Lee, B. K. (1965). Genetic analysis of actidione resistance in Saccharomyces cerevisiae. Genet. Res. 6, 130138.CrossRefGoogle ScholarPubMed