Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-26T14:06:11.940Z Has data issue: false hasContentIssue false

Genetic analysis of actidione resistance in Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

D. Wilkie
Affiliation:
Department of Botany, University College London
B. K. Lee
Affiliation:
Department of Botany, University College London
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Individual genes of a multigenic system of resistance to actidione in yeast were analysed in a series of crosses involving resistant strains and sensitive strains.

2. Resistance conferred varied in the range 0·5 p.p.m. to 20 p.p.m.

3. Positive interaction was seen between resistance genes in recombinant strains.

4. Recessive modifier genes were found which do not themselves confer resistance but which modify the activity of specific resistance genes in a positive way, that is towards a greater degree of resistance. Both a two-fold and a five-fold increase in resistance is seen depending on the gene combination.

5. In selection experiments, strains resistant to relatively high concentrations of actidione (1000 p.p.m.) were obtained but only by selection on media containing successively higher concentrations of the drug, that is, by the step wise build up of multiply mutant forms. Mutations of interacting resistance genes and specific modifier genes were obtained by selection methods including pretreatment of cells with ultra-violet light.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Bryan, B. E. (1961). Genetic modifiers of streptomycin resistance in Pneumococcus. J.Bact. 82, 461470.CrossRefGoogle ScholarPubMed
Cavalli, L. L. & Maccacaro, G. A. (1952). Polygenic inheritance of drug resistance in the bacterium Escherichia coli. Heredity, 6, 311331.CrossRefGoogle Scholar
Ford, J. H. & Leach, B. E. (1948). Actidione, an antibiotic from Streptomyces griseus. J. Amer. chem. Soc. 70, 12231225.CrossRefGoogle ScholarPubMed
Hsu, K. S. (1963). The genetic basis of resistance to actidione in Neurospora. J. gen. Microbiol. 32, 341347.CrossRefGoogle ScholarPubMed
Lee, B. K. & Wilkie, D. (1964). Sensitivity and resistance of yeast strains to actidione and actidione derivatives. Nature (in press).Google Scholar
Middlekauf, J. E., Hino, S., Yang, S. P., Lindegren, C. C. & Lindegren, G. (1957). Gene control of resistance vs. sensitivity to actidione in Saccharomyces. Genetics, 42, 6671.CrossRefGoogle Scholar
Warr, J. R. & Roper, J. A. (1964). Resistance mutations of Aspergillus nidulans. Heredity (in press).Google Scholar
Whiffen, A. J., Bohonos, N. & Emerson, R. L. (1946). The production of an antifungal antibiotic by Streptomyces griseus. J. Bact. 52, 610611.CrossRefGoogle ScholarPubMed
Wickerham, L. J. (1946). A critical evaluation of the nitrogen assimilation tests commonly used in the classification of yeasts. J. Bact. 52, 293301.CrossRefGoogle ScholarPubMed
Wilkie, D. (1963). The induction by monochromatic uv light of respiratory deficient mutants in aerobic and anaerobic cultures of yeast. J. Mol. Biol. 7, 527533.CrossRefGoogle ScholarPubMed