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A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia

Published online by Cambridge University Press:  14 April 2009

Jennifer Dee  and
Russell T. M. Poulter
Jennifer Dee
Affiliation:
Department of Genetics, University of Leicester, England
Russell T. M. Poulter
Affiliation:
Department of Genetics, University of Leicester, England

Summary

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Plasmodia of P. polycephalum homozygous and heterozygous for a mutation (act) which confers resistance to actidione on the haploid amoebal stage have been tested for resistance to actidione in liquid semidefined medium. Growth of homozygous resistant (act/act) plasmodia determined by protein or pigment assay was inhibited less by actidione than growth of homozygous-sensitive or heterozygous plasmodia. The mutant (act) is therefore recessive but, contrary to a previous report, confers resistance on homozygous plasmodia. The same mutation affects the morphology of homozygous (act/act) plasmodia on agar medium, causing slow growth, fragmentation and excessive sliminess.

Type
Research Article
Information
Genetics Research , Volume 15 , Issue 1 , February 1970 , pp. 35 - 41
Copyright
Copyright © Cambridge University Press 1970

References

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