Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-08T07:50:43.795Z Has data issue: false hasContentIssue false
  • English
  • Français

Further studies on the maintenance of mate-killer (mu) particles in Stock 540 Paramecium aurelia

Published online by Cambridge University Press:  14 April 2009

J. Franklin
J. Franklin
Affiliation:
School of Biological Sciences, University of East Anglia, Norwich, NOR 88C*
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

1. Mu particles can be removed from stock 540 Paramecium aurelia by incubation in a solution of 1·0 mg/ml RNAase for 12 h. Only a relatively small percentage of cells become mu-free and it is suggested that this variation is due to the activity of the M genes producing metagons at different times.

2. Paramecia, made mu-free by growth at elevated temperatures, can be re-infected with mu by allowing the cells to swim in a brei of stock 540 paramecia. The re-infection is only permanently successful with cells that were made mu-free 10 days earlier.

Type
Research Article
Information
Genetics Research , Volume 22 , Issue 2 , October 1973 , pp. 135 - 141
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Balbinder, E. (1957). Two loci controlling the maintenance and stability of the cytoplasmic factor ‘kappa’ in stock 51, var. 4, killers of Paramecium aurelia. Genetics 31, 634.Google Scholar
Balbinder, E. (1959). The genetic control of kappa in Paramecium aurelia syngen 4, stock 51. Genetics 44, 1227–1241.Google Scholar
Ball, G. H. (1967). Organisms living on and in protozoa. In Research in Protozoology, Vol. 3 (ed. Chen, T. T.), pp. 566–718. London: Pergamon.Google Scholar
Beale, G. H. (1957). A mate-killing strain of Paramecium aurelia, variety 1, from Mexico. Proceedings of the Royal Physical Society Edinburgh 26, 11–14.Google Scholar
Beale, G. H. & Jurand, A. A. (1966). Three different types of mate-killer (mu) particles in P. aurelia (syngen 1). Journal of Cell Science 1, 31–34.Google Scholar
Beale, G. H. & McPhail, S. (1967). Some additional results on the maintenance of kappa in stock 51 paramecia, after the loss of the K gene. Genetical Research 9, 369–375.CrossRefGoogle Scholar
Byrne, B. J. (1969). Kappa, mu and the metagon hypothesis in Paramecium aurelia. Genetical Research 13, 197–211.Google Scholar
Dryl, S. (1959). Antigenic transformation in Paramecium aurelia after homologous antiserum treatment during autogamy and conjugation. Journal of Protozoology 6 (supplement), 225.Google Scholar
Franklin, J. (1971). Studies on the mu particle of Paramecium aurelia. Ph.D. Thesis, University of East Anglia.Google Scholar
Franklin, J. (1973). The removal of mu particles from Paramecium aurelia (stock 540) by penicillin. Journal of General Microbiology 74, 175–177.Google Scholar
Gibson, I. (1965). The replication of metagons and mu particles from Paramecium aurelia in another cell – Didinium. Genetical Research 6, 198–410.CrossRefGoogle Scholar
Gibson, I. & Beale, G. H. (1961). Genetical basis of the mate-killer trait in Paramecium aurelia, stock 540. Genetical Research 2, 82–91.CrossRefGoogle Scholar
Gibson, I. & Beale, G. H. (1962). The mechanism whereby the genes Ml and M2 in Paramecium aurelia, stock 540, control growth of the mate-killer (mu) particles. Genetical Research 3, 24–50.Google Scholar
Gibson, I. & Beale, G. H. (1963). The action of ribonuclease and 8-azaguanine on mate-killer paramecia. Genetical Research 4, 42–54.Google Scholar
Gibson, I. & Beale, G. H. (1964). Infection into paramecia of metagons derived from other mate-killer paramecia. Genetical Research 5, 85–106.Google Scholar
Gibson, I. & Sonneborn, T. M. (1964). Is the metagon an mRNA in Paramecia and a virus in Didinium? Proceedings of the National Academy of Sciences, U.S. 52, 869–876.Google Scholar
Gibson, I., Williams, J. & Chance, M. (1971). Extranuclear DNA and the endosymbionts of Paramecium aurelia. Nature, New Biology, London 234, 75–77.CrossRefGoogle ScholarPubMed
Jurand, A., Gibson, I. & Beale, G. H. (1962). The action of ribonuclease on living paramecia. Experimental Cell Research 26, 598–600.Google Scholar
Preer, J. R. (1967). Genetics of protozoa. In Research in Protozoology, Vol. 3 (ed. Chen, T. T.), pp. 133–278. London: Pergamon.Google Scholar
Reeve, E. C. R. & Ross, G. J. S. (1963). Mate-killer (mu) particles in P. aurelia: the metagon division hypothesis. Genetical Research 4, 158–161.Google Scholar
Sonneborn, T. M. (1950). Methods in the general biology and genetics of Paramecium aurelia. Journal of Experimental Zoology 113, 87–143.Google Scholar
Sonneborn, T. M. (1959). Kappa and related particles in Paramecium. Advances in Virus Research 6, 229–356.CrossRefGoogle Scholar
Widmayer, D. J. (1966). Search for metagon activity for kappa's maintenance in stock 51m 43 of Paramecium aurelia. American Zoologist 6, 589–594.Google Scholar
Yeung, K. K. (1965). Maintenance of kappa particles in cells recently deprived of gene K (stock 51, syngen 4) of Paramecium aurelia. Genetical Research 6, 411–418.Google Scholar