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Further observations on intragenic recombination in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Arthur J. Hilliker  and
Arthur Chovnick
Arthur J. Hilliker
Affiliation:
Genetics and Cell Biology Section, Biological Sciences Group, The University of Connecticut, Storrs, Connecticut 06268
Arthur Chovnick
Affiliation:
Genetics and Cell Biology Section, Biological Sciences Group, The University of Connecticut, Storrs, Connecticut 06268
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This report examines several issues bearing upon intragenic recombination in higher eukaryotes. The fine structure data accumulated in our analysis of the genetic organization of the rosy locus in Drosophila melanogaster. Firstly, we confirm that a conversion event has a markedly less than 50% probability of resulting in flanking marker exchange, a finding consistent with more recent analyses of the available Saccharomyces data (e.g. Fogel et al. 1978). As reported earlier, co-conversion of recombinationally separable sites within the rosy locus occurs (McCarron, Gelbart & Chovnick, 1974). In this report, we demonstrate that the frequency of co-conversion is inversely proportional to the distance between co-converting sites. As in fungi, real conversion frequency differences are observed among rosy mutant alleles, and the data suggest that there may be a relationship between allele conversion frequency and map position. Unlike Neurospora and Saccharomyces, only one flanking marker exchange class is recovered from any given mutant heteroallele recombination experiment. In this respect, the Drosophila system resembles Aspergillus. As in Neurospora and Saccharomyces, rosy locus intragenic recombinants associated with flanking marker exchange exhibit interference with crossing over in adjacent regions, while no interference is seen among recombinants exhibiting parental flanking markers. Finally, experimental results are discussed which demonstrate the occurrence of postmeiotic segregation in Drosophila. These analogies between Drosophila and fungi provide further evidence in support of the notion that eukaryotes share common molecular mechanism(s) of meiotic recombination.

Type
Research Article
Information
Genetics Research , Volume 38 , Issue 3 , December 1981 , pp. 281 - 296
Copyright
Copyright © Cambridge University Press 1981

References

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