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The extended survival of tw5/tw5 mouse embryo cells in vitro

Published online by Cambridge University Press:  14 April 2009

William Garside
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122, USA
Nina Hillman*
Affiliation:
Department of Biology, Temple University, Philadelphia, PA 19122, USA
*
* Corresponding author.
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Summary

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The tw5 haplotype is a recessive mutation which is lethal when homozygous in mouse embryos following implantation. This series of studies was undertaken to determine the effect of the tw5/tw5 genotype on embryos developing in vitro. Blastocyst embryos from + / tw5inter se matings were compared with control blastocysts obtained from matings between T/ + and + / + females and + / tw5 males for their abilities to continue development in vitro in two culture media. The data show that there are no significant differences between the percentages of experimental and control blastocyst embryos which attach and outgrow or which contain inner cell masses on any day of culture up to equivalent gestation day 21 in either media. These findings show that the life span of cells from tw5 / tw5 embryos can be extended significantly by in vitro culture.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1990

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