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exrB: a malB-linked gene in Escherichia coli B involved in sensitivity to radiation and filament formation

Published online by Cambridge University Press:  14 April 2009

Joseph Greenberg ,
Leonard J. Berends ,
John Donch  and
Michael H. L. Green
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Leonard J. Berends
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
John Donch
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Michael H. L. Green
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
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Summary

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PAM 26, a radiation-sensitive mutant of Escherichia coli strain B, is described. Its properties are attributable to a mutation in a gene, exrB, which is cotransducible with malB. It differs from uvrA (also malB-linked) derivatives of strain B in being sensitive to 1-methyl-3-nitro-1-nitroso-guanidine and γ-radiation, and in being able to reactivate UV-irradiated phage T3. It differs from exrA (also malB-linked) derivatives of strain B in forming filaments during the course of normal growth as well as after irradiation. When exrB was transduced into a K12 (lon+) strain, filaments did not form spontaneously. Three-point transductions established the order of markers as met A malB exrB. Based on an analysis of the frequency of wild-type recombinants in a reciprocal transduction between exrA and exrB strains, it was inferred that they are not isogenic and that the order of markers is malB exrA exrB.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 2 , April 1974 , pp. 175 - 184
Copyright
Copyright © Cambridge University Press 1974

References

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