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Evidence for random distribution of sequence variants in Tenebrio molitor satellite DNA

Published online by Cambridge University Press:  14 April 2009

Miroslav Plohl ,
Branko Borˇstnik ,
Vlatka Lucijanić-Justić  and
ÐurÐica Ugarković
Miroslav Plohl
Affiliation:
Ruder Boškovic Institute, Bijenićrka 54, PO Box 1016, 41000 Zagreb, Croatia
Branko Borˇstnik
Affiliation:
Boris Kidrič Institute of Chemistry, Hajdrihova 19, PO Box 30, 61115 Ljubljana, Slovenia
Vlatka Lucijanić-Justić
Affiliation:
Ruder Boškovic Institute, Bijenićrka 54, PO Box 1016, 41000 Zagreb, Croatia
ÐurÐica Ugarković
Affiliation:
Ruder Boškovic Institute, Bijenićrka 54, PO Box 1016, 41000 Zagreb, Croatia

Summary

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Tenebrio molitor satellite DNA has been analysed in order to study sequential organization of tandemly repeated monomers, i.e. to see whether different monomer variants are distributed randomly over the whole satellite, or clustered locally. Analysed sequence variants are products of single base substitutions in a consensus satellite sequence, producing additional restriction sites. The ladder of satellite multimers obtained after digestion with restriction enzymes was compared with theoretical calculations and revealed the distribution pattern of particular monomer variants within the satellite. A defined higher order repeating structure, indicating the existence of satellite subfamilies, could not be observed. Our results show that some sequence variants are very abundant, being present in nearly 50 % of the monomers, while others are very rare (0-1 % of monomers). However, the distribution of either very frequent, or very rare sequence variants in T. molitor satellite DNA is always random. Monomer variants are randomly distributed in the total satellite DNA and thus spread across all chromosomes, indicating a relatively high rate of sequence homogenization among different chromosomes. Such a distribution of monomer variants represents a transient stage in the process of sequence homogenization, indicating the high rate of spreading in comparison with the rate of sequence variant amplification.

Type
Research Article
Information
Genetics Research , Volume 60 , Issue 1 , August 1992 , pp. 7 - 13
Copyright
Copyright © Cambridge University Press 1992

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