No CrossRef data available.
Article contents
Effects of the white allele of the mi locus on coat pigmentation in chimeric mice
Published online by Cambridge University Press: 14 April 2009
Summary
Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
To investigate the cellular action of the Miωh allele in the mouse with regard to its effects upon coat color patterns, we generated a series of aggregation chimeras, using embryos that differ in their mi locus genotype. We have obtained 11 chimeras Miωh/ + C/C↔ + / + c/c and 8 chimeras + / + C/C↔ + / + c/c. Chimerism was determined by coat and retinal pigment epithelium mosaicism and by the electrophoretic analysis of GPI-1 isoenzymes. In Miωh/+ C/C↔, +/+ c/c mice white coat color prevailed due to the higher percentage of unpigmented areas and the higher percentage of unpigmented hairs in pigmented areas. Our data indicate that a single Miωh gene dose decreases the melanoblast proliferative activity, causing the lightening of coat pigmentation. In Miωh/ + C/C↔+/+ c/c mice a few pigmented hairs were often detected on the belly where Miωh / + mice always had a white spot. This suggests that in the chimeras the presence of some non-Miωh cells in the skin of the belly allows pigment cells to develop. Using embryos of two substrains of Miωh/Miωh mice that differ in their Gpi-1 locus genotype we have produced 8 Miωh/ + ↔ Miωh/Miωh chimeras. In all these chimeras coat color patterns resembled those of Miωh/ + heterozygotes despite the higher percentage of the Miwh/Miωh component in three chimeras. Mosaic hairs were absent in the chimeras. This shows that the chimeras have only one Miωh/ + melanoblast population which actively proliferates and colonizes almost all hair follicles. Thus the Miωh/Miωh dermis and epidermis do not suppress proliferation and differentiation of the Miωh/ + melanoblasts except the certain area on the belly.
- Type
- Research Article
- Information
- Copyright
- Copyright © Cambridge University Press 1994
References
Gray, P. (Ed) (1973). The Encyclopedia of Microscopy and Microtechnique p. 338. Cincinnati, Toronto, London, Melbourne: Van Nostrand Reinhold.Google Scholar
Green, M. C. (1989). Catalog of mutant genes and polymorphic loci. In Genetic Variants and Strains of the Laboratory Mouse, 2nd edn. (ed. Lyon, M. F., and Searle, A. G.), pp. 12–404. New York: Oxford University Press.Google Scholar
Huszar, D., Sharpe, A. & Jaenisch, R. (1991). Migration and proliferation of cultured neural crest cells in W mutant neural crest chimeras. Development 112, 131–141.CrossRefGoogle Scholar
Kindyakov, B. N., Konyukhov, B. V. & Malinina, N. A. (1984). Study of mutant gene expression in mouse aggregation chimeras. 1. The white gene. Ontogenez 15, 153–162. (in Russian).Google Scholar
Konyukhov, B. V., Kindyakov, B. N., Malinina, N. A. & Sazhina, M. V. (1986). A study of mutant gene expression in mouse aggregation chimeras. 6. Analysis of the white gene effects by a histochemical marker. Ontogenez 17, 389–395. (in Russian).Google Scholar
Konyukhov, B. V. & Osipov, V. V. (1968). Interallelic complementation of the genes microphthalmia and white in mouse. Genetika 4(11), 65–75. (in Russian).Google Scholar
Konyukhov, B. V. & Sazhina, M. V. (1980). Allelic complementation at the mi-locus of the mouse: partial normalization of the retinal pigment epithelium development. Biologisches Zentralblatt 99, 571–584.Google Scholar
Markert, C. L. & Silvers, W. K. (1956). The effects of genotype and cell environment on melanoblast differentiation in the house mouse. Genetics 41, 429–450.CrossRefGoogle ScholarPubMed
Mayer, T. C. & Green, M. C. (1968). An experimental analysis of the pigment defect caused by mutations at the W and Sl loci in mice. Developmental Biology 18, 62–75.CrossRefGoogle Scholar
Mintz, B. (1967). Gene control of mammalian pigmentary differentiation. 1. Clonal origin of melanocytes. Proceedings of the National Academy of Sciences U.S.A. 58, 344–351.Google ScholarPubMed
Mintz, B. (1969). Gene control of the mouse pigmentary system. Genetics 61, (Supplement), 41.Google Scholar
Mintz, B. (1970). Gene expression in allophenic mice. In Control Mechanisms in the Expression of Cellular Phenotypes (ed. Padykula, H. A.), pp. 15–42. New York: Academic Press.CrossRefGoogle Scholar
Mintz, B. (1971). Allophenic mice of multi-embryo origin. In Methods in Mammalian Embryology, (eds. Daniel, J. C.), pp. 186–214. San Francisco: Freeman.Google Scholar
Mintz, B., Gearhart, Y. D. & Guymont, A. O. (1973). Phytohemagglutinin mediated blastomere aggregation and development of allophenic mice. Developmental Biology 31, 195–199.CrossRefGoogle ScholarPubMed
Nakayama, H., Ru, X.-M., Fujita, J., Kasugai, T., Onoue, H., Hirote, S., Kuroda, H. & Kitamura, Y. (1990). Growth competition between W mutant and wild-type cells in mouse aggregation chimeras. Development, Growth and Differentiation 32, 255–261.CrossRefGoogle ScholarPubMed
Pratt, B. M. (1982). Site of gene action of the white allele (Miwh) of the microphthalmia locus: a dermal-epidermal recombination study. Journal of Experimental Zoology 220, 93–101.CrossRefGoogle ScholarPubMed
Pratt, B. M. (1983). Effects of microphthalmic white (Miwh) on the number function of cutaneous melanocytes. Journal of Experimental Zoology 225, 329–335.CrossRefGoogle ScholarPubMed
Rawles, M. E. (1955). Pigmentation in autoplastic and homoplastic grafts of skin from fetal and newborn hooded rats. American Journal of Anatomy 97, 79–128.CrossRefGoogle ScholarPubMed
Schaible, R. (1968). Development of transitory piebald spotted and completely pigmented patterns in the chicken. Developmental Biology 18, 117–148.CrossRefGoogle ScholarPubMed
Schaible, R. H. (1969). Clonal distribution of melanocytes in piebald-spotted and variegated mice. Journal of Experimental Zoology 172, 181–199.CrossRefGoogle ScholarPubMed
Shows, T. B. & Ruddle, F. H. (1968). Malate dehydrogenase: Evidence for tetrameric structure in Mus musculus. Science 160, 1356–1357.CrossRefGoogle ScholarPubMed
Silvers, W. K. (1956). Pigment cells: occurrence in hair follicles. Journal of Morphology 99, 41–55.CrossRefGoogle Scholar
Stephenson, D. A., Glenister, P. H. & Hornby, J. E. (1985). Site of the beige (bg) and leaden (ln) pigment gene expression determined by recombinant embryonic skin grafts and aggregation mouse chimeras employing sash (Wsh) homozygotes. Genetical Research 46, 193–20.CrossRefGoogle ScholarPubMed
You have
Access