Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-20T13:33:20.127Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of actinomycin D and puromycin upon excystment of Didinium nasutum

Published online by Cambridge University Press:  14 April 2009

H. M. Butzel  and
L. Mayer
H. M. Butzel
Affiliation:
Department of Biological Sciences, Union College, Schenectady, New York 12308, U.S.A.
L. Mayer
Affiliation:
Department of Biological Sciences, Union College, Schenectady, New York 12308, U.S.A.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Activated cysts of Didinium nasutum continue excystment after treatment with actinomycin D or puromycin. Cysts treated at developmental stages 19–20 emerge holociliated and lacking the seizing organ. No effect of the antibiotics at any other stages was found, suggesting that it is at stage 19–20 that the initiation of normal ciliation and of mouth formation occurs. Treatment with other metabolic inhibitors either blocks excystment completely or has no effect on normal development depending upon dosage, suggesting that the action of the antibiotics is specific.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 291 - 296
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Beers, C. D. (1935). Structural changes during encystment and excystment in the ciliate Didinium nasutum. Archives für Protistenkunde 84, 133–155.Google Scholar
Beers, C. D. (1945). The excystment process in the ciliate Didinium nasutum. Journal of the Elisha Mitchell Society 61, 264–275.Google Scholar
Beers, C. D. (1946). Excystment in Didinium nasutum with special references to the role of bacteria. Journal of Experimental Zoology 103, 201–231.CrossRefGoogle Scholar
Butzel, H. M. & Bolten, A. B. (1968). The relationship of the nutritive state of the prey organism Paramecium aurelia to the growth and development of Didinium nasutum. Journal of Protozoology 15, 256–258.CrossRefGoogle Scholar
Butzel, H. M. & Davich, M. (1974). The relationship of the cyst walls to excystment of Didinium nasutum as demonstrated by various agents acting upon these walls. Journal of Protozoology 21 (suppl), 441.Google Scholar
Butzel, H. M. & Horwitz, H. (1965). Excystment of Didinium nasutum. Journal of Protozoology 12, 413–416.CrossRefGoogle ScholarPubMed
Butzel, H. M. & Mayer, L. (1974). Effects of actinomycin D and puromycin upon excystment of Didinium nasutum. Journal of Protozoology 21 (suppl), 419.Google Scholar
Galtsoff, P. S., Lutz, F. E., Welch, P. S. & Needham, J. G. (1937). Culture Methods for Invertebrate Animals, p. 101. Ithaca, New York: Comstock Publishing Company.Google Scholar
Hansok, E. D. & Kaneda, M. (1968). Evidence for sequential gene action within the cell cycle of Paramecium aurelia. Genetics 60, 793–805.CrossRefGoogle Scholar
Holt, P. A. (1972). An electron microscope study of the Rhabdophorine ciliate, Didinium nasutum, during excystment. Transactions of the American Microscopical Society. 191, 144–168.CrossRefGoogle Scholar
James, E. (1967). Regeneration and division in Stentor coeruleus: the effects of microinjected and externally applied actinomycin D and puromycin. Developmental Biology 16, 577–593.CrossRefGoogle ScholarPubMed
Rieder, N. (1973). Elektronenoptische und histochemische Untersuchungen an der Cystenhülle von Didinium nasutum O. F. Müller. Archives für Protistenkunde 115, 125–131.Google Scholar
Sapra, G. R. & Ammerman, D. (1974). An analysis of the developmental program in relation to RNA metabolism in the ciliate Stylonichia mytilus. Developmental Biology 36, 105–112.CrossRefGoogle Scholar
Whitson, G. L. & Padilla, C. M. (1965). The effects of actinomycin D on stomatogenesis and cell division in temperature-synchronized Tetrahymena pyriformis. Experimental Cell Research 36, 667–671.CrossRefGoogle Scholar