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Effects of actinomycin D and puromycin upon excystment of Didinium nasutum

Published online by Cambridge University Press:  14 April 2009

H. M. Butzel  and
L. Mayer
H. M. Butzel
Affiliation:
Department of Biological Sciences, Union College, Schenectady, New York 12308, U.S.A.
L. Mayer
Affiliation:
Department of Biological Sciences, Union College, Schenectady, New York 12308, U.S.A.

Summary

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Activated cysts of Didinium nasutum continue excystment after treatment with actinomycin D or puromycin. Cysts treated at developmental stages 19–20 emerge holociliated and lacking the seizing organ. No effect of the antibiotics at any other stages was found, suggesting that it is at stage 19–20 that the initiation of normal ciliation and of mouth formation occurs. Treatment with other metabolic inhibitors either blocks excystment completely or has no effect on normal development depending upon dosage, suggesting that the action of the antibiotics is specific.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 291 - 296
Copyright
Copyright © Cambridge University Press 1976

References

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