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Effective method for the isolation of non-nodulating mutants of Rhizobium trifolii

Published online by Cambridge University Press:  14 April 2009

Witold Żurkowski  and
Zbigniew Lorkiewicz
Witold Żurkowski
Affiliation:
Institute of Microbiology, University M. Curie-Sklodowska, Akademicka 19, 20–033 Lublin, Poland
Zbigniew Lorkiewicz
Affiliation:
Institute of Microbiology, University M. Curie-Sklodowska, Akademicka 19, 20–033 Lublin, Poland
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Non-nodulating mutants of Rhizobium trifolii were found in cultures incubated at 35 °C for 7 days. From among ten strains incubated at the elevated temperature six became non-nodulating. The frequency of Nod− mutants in R. trifolii cultures varied from 1 to 75% depending on the strain tested. It is suggested that gene(s) controlling nodulation are located on a large plasmid.

Type
Short Paper
Information
Genetics Research , Volume 32 , Issue 3 , November 1978 , pp. 311 - 314
Copyright
Copyright © Cambridge University Press 1978

References

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