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Effect of inhibition of DNA synthesis on mating in Escherichia coli K12

Published online by Cambridge University Press:  14 April 2009

Susan Hollom  and
R. H. Pritchard
Susan Hollom
Affiliation:
Department of Genetics, The University, Leicester
R. H. Pritchard
Affiliation:
Department of Genetics, The University, Leicester
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From studies involving inhibition of DNA synthesis in Hfr strains of Escherichia coli K12, either by thymine starvation (Pritchard, 1963) or amino-acid starvation (Suit, Matney, Doudney & Billen, 1964), during mating with F− strains, it has been concluded that transfer of DNA from males to females can occur in the absence of DNA synthesis. This conclusion is at variance with the hypothesis (Jacob, Brenner & Cuzin, 1963) that the energy required for transfer is derived from the process of DNA replication. On the other hand, a second prediction from this hypothesis, that one polynucleotide chain of the DNA transferred during mating will have been synthesized during transfer, is strongly supported by recent experiments (Ptashne, 1965; Blinkova, Bresler & Lanzov, 1965; Gross & Caro, 1965).

Type
Short Notes
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 479 - 483
Copyright
Copyright © Cambridge University Press 1965

References

REFERENCES

Blinkova, A. A., Bresler, S. E. & Lanzov, V. A. (1965). DNA synthesis and chromosome transfer in E. coli K12. Z. VererbLehre, 96, 267–274.Google Scholar
Goss, W. A., Deitz, W. H. & Cook, T. M. (1964). Mechanism of action of Nalidixic acid on E. coli. J. Bact. 88, 1112–1118.CrossRefGoogle Scholar
Goss, W. A., Deitz, W. H. & Cook, T. M. (1965). Mechanism of action of Nalidixic acid on E. coli II. J. Bact. 89, 1068–1074.CrossRefGoogle Scholar
Gross, J. D. & Caro, L. (In press.)Google Scholar
Jacob, F., Brenner, S. & Cuzin, F. (1963). On the regulation of DNA replication in bacteria. Cold Spring Harb. Symp. quant. Biol. 28, 329–348.CrossRefGoogle Scholar
Pritchard, R. H. (1964). The relationship between conjugation recombination and DNA synthesis in E. coli. Proc. XI Int. Congr. Genet. in Genetics Today, pp. 55–78. London: Pergamon Press.Google Scholar
Ptashne, M. (1965). Replication and host modification of DNA transferred during bacterial mating. J. molec. Biol. 11, 829–838.CrossRefGoogle ScholarPubMed
Roodyn, D. B. & Mandel, H. G. (1960). A simple membrane fractionation method for determining the distribution of radio-activity in chemical fractions of Bacillus cereus. Biochim. biophys. Acta, 41, 80–88.CrossRefGoogle Scholar
Suit, J. C., Matney, T. S., Doudney, C. O. & Billen, D. (1964). Transfer of the E. coli K12 chromosome in the absence of DNA synthesis. Biochem. biophys. res. Commun. 17, 237–241.CrossRefGoogle Scholar