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The dominance of exrB over exrB+ in heterodiploids of Escherichia coli*

Published online by Cambridge University Press:  14 April 2009

Joseph Greenberg ,
John Donch  and
Leonard Berends
Joseph Greenberg
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
John Donch
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
Leonard Berends
Affiliation:
Palo Alto Medical Research Foundation, Palo Alto, California
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To test whether exrB, a mutation in Escherichia coli B making this strain sensitive to ultraviolet radiation and unable to divide normally, is dominant to exrB+, merodiploids were constructed by crossing Hfr exrB metA+his × F−malB met A thr proA recA. metA+recombinants were fertile (F′) and sensitive to UV. One of these, F′ exrB proA, was crossed with an F−metA malB thr, selecting for metA+proA+. All such progeny acquiring malB+ were UV-sensitive, fertile and segregated met mal UV-resistant progeny. They were, therefore, meriodiploids, F′ exrB/exrB+. exrB is dominant to exrB+, in which respect it resembles exrA (lex).

Type
Research Article
Information
Genetics Research , Volume 25 , Issue 1 , February 1975 , pp. 39 - 44
Copyright
Copyright © Cambridge University Press 1975

References

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