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Direct and correlated responses to artificial selection on lipid and glycogen contents in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

Andrew G. Clark ,
Frances M. Szumski ,
Karen A. Bell ,
Lisa Esterling Keith ,
Sharyn Houtz  and
D. Andrew Merriwether
Andrew G. Clark*
Affiliation:
Department of Biology and Genetics Program, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802
Frances M. Szumski
Affiliation:
Department of Biology and Genetics Program, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802
Karen A. Bell
Affiliation:
Department of Biology and Genetics Program, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802
Lisa Esterling Keith
Affiliation:
Department of Biology and Genetics Program, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802
Sharyn Houtz
Affiliation:
Department of Biology and Genetics Program, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802
D. Andrew Merriwether
Affiliation:
Department of Biology and Genetics Program, 208 Mueller Laboratory, Pennsylvania State University, University Park, PA 16802
*
Dr Andrew G. Clark.
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A large outbred population of Drosophila melanogaster was subjected to artificial selection on lipid and glycogen storage. In three separate experiments, two replicates underwent sib selection for both increased and decreased storage. In the first study, flies were selected on the basis of total triacylglycerol for ten generations. This experiment resulted in no significant direct response, but there was a significant change in total body weight, underscoring the importance of concern for the allometric relationship between body weight and lipid content. In the second study, selection was performed for 15 generations on the percentage of body composition that was triacylglycerol. A significant direct response was obtained, and the two replicates revealed heritability estimates of 0·40 and 0·43. The third study selected glycogen content for 15 generations, and produced a significant response with heritabilities of 0·25 and 0·31. A series of 12 biochemical and enzyme kinetic traits was examined at five generation intervals in all experiments, and a number of correlated responses were detected. The results are interpreted with respect to the evolutionary constraints on energy storage evolution and the genetic basis of the allometric relationship between body weight and fat content.

Type
Research Article
Information
Genetics Research , Volume 56 , Issue 1 , August 1990 , pp. 49 - 56
Copyright
Copyright © Cambridge University Press 1990

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