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Development of Drosophila on sterol mutants of the yeast Saccharomyces cerevisiae

Published online by Cambridge University Press:  14 April 2009

Marten Bos ,
Barrie Burnet ,
Roy Farrow  and
Robin A. Woods
Marten Bos
Affiliation:
Department of Genetics, University of Sheffield, England
Barrie Burnet
Affiliation:
Department of Genetics, University of Sheffield, England
Roy Farrow
Affiliation:
Department of Genetics, University of Sheffield, England
Robin A. Woods
Affiliation:
Department of Genetics, University of Sheffield, England
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Four species of Drosophila (melanogaster, simulans, mauritiana and virilis) were tested for their ability to survive on erg strains of Saccharomyces cerevisiae each having a mutant block at a different reaction in the synthesis of ergosterol. Species capable of completing development on a given yeast mutant strain were tested for egg production and viability, and also for their ability to survive on the mutant yeast for five complete generations. Sterol analyses using gas–liquid chromatography show that the sterol composition of flies closely resembles that of the yeasts on which they are grown, confirming that the steric criteria for sterols capable of a structural role in lipoprotein membranes are relatively broad. Even so, different sterols are not equally efficient in this respect and there are differences between species in their tolerance to the sterols available from each of the yeast mutants. The range of sterols capable of satisfying the micronutrient, as distinct from the macronutrient, requirement is narrower. Growth on two of the yeast mutants (erg-2 and erg-6) leads to developmental arrest in the larval stage due, it is suggested, to a block in the synthesis of ecdysone. The four Drosophila species lack an effective level of Δ8−Δ7 isomerase which is necessary for utilization of zymosterol and other Δ8 sterol precursors of ergosterol. Ergosta-5,7-diene-3β-ol appears to be capable of substituting for cholesterol in the metabolism of Drosophila.

Type
Research Article
Information
Genetics Research , Volume 28 , Issue 2 , October 1976 , pp. 163 - 176
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Barton, D. H. R., Corrie, J. E. T., Widdowson, D. A., Bard, M. & Woods, R. A. (1974). Biosynthesis of terpenes and steroids. IX. The sterols of some mutant yeasts and their relationship to the biosynthesis of ergosterol. Journal of the Chemical Society. Perkin Transactions 1, 1326–1333.Google Scholar
Barton, D. H. R., Gunatilaka, A. A. L., Jarman, T. R., Widdowson, D. A., Bard, M. & Woods, R. A. (1975). Biosynthesis of terpenes and steroids. X. The sterols of some yeast mutants doubly defective in ergosterol biosynthesis. Journal of the Chemical Society. Perkin Transactions 1, 88–92.Google Scholar
Breivik, O. N. & Owades, J. L. (1957). Spectrophotometric semi-microdetermination of ergosterol in yeast. Agricultural Food Chemistry 5, 360–363.CrossRefGoogle Scholar
Clayton, R. B. (1964). The utilization of sterols by insects. Journal of Lipid Research 5, 3–19.CrossRefGoogle ScholarPubMed
Cooke, J. & Sang, J. H. (1970). Utilization of sterols by larvae of Drosophila melanogaster. Journal of Insect Physiology 16, 801–812.CrossRefGoogle ScholarPubMed
Erk, F. C. & Sang, J. H. (1966). The comparative nutritional requirements of two sibling species Drosophila simulans and Drosophila melanogaster. Journal of Insect Physiology 12, 43–51.CrossRefGoogle Scholar
Heed, W. B. & Kircher, H. W. (1965). Unique sterol in the ecology and nutrition of Drosophila pachea. Science 149, 758–761.CrossRefGoogle ScholarPubMed
Kircher, H. W. (1969). Sterols in the leaves of the Cheirodendron gaudichaudii tree and their relationship to Hawaiian Drosophila ecology. Journal of Insect Physiology 15, 1167– 1173.CrossRefGoogle Scholar
Molzahn, S. W. & Woods, R. A. (1972). Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae. The Journal of General Microbiology 72, 339–348.CrossRefGoogle ScholarPubMed
Robertson, F. W. & Reeve, E. C. R. (1952). Studies in quantitative inheritance. I. The effects of selection of wing and thorax length in Drosophila melanogaster. Journal of Genetics 50, 414–448.Google Scholar
Robertson, F. W., Shook, M., Takei, G. & Gaines, H. (1968). Observations on the biology and nutrition of Drosophila disticha, Hardy, an indigenous Hawaiian species. University of Texas Publication, no. 6818, pp. 279–299.Google Scholar
Royes, W. V. & Robertson, F. W. (1964). The nutritional requirements and growth relations of different species of Drosophila. Journal of Experimental Zoology 156, 105–135.CrossRefGoogle Scholar
Sang, J. H. (1972). Utilization of lipids by Drosophila melanogaster. In Insect and Mite Nutrition (Ed. Rodriguez, J. G.), pp. 493–503. Amsterdam: North-Holland.Google Scholar
Sang, J. H. & King, R. C. (1961). Nutritional requirements of axenically cultured Drosophila melanogaster adults. Journal of Experimental Biology 38, 793–809.CrossRefGoogle Scholar
Thompson, M. J., Svoboda, J. A., Kaplanis, J. N. & Robins, W. E. (1972). Metabolic pathways of steroids in insects. Proceedings of the Royal Society of London B 180, 203–221.Google ScholarPubMed
Woods, R. A., Bard, M., Gardner, I. E. & Molzahn, S. W. (1974). Studies on the accumulation of ergosterol and 24(28)-dehydroergosterol in 3 strains of Saccharomyces cerevisiae. Microbios 10 A, 73–80.Google Scholar