Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-23T00:42:20.588Z Has data issue: false hasContentIssue false
  • English
  • Français

Demonstration of different mutational sites controlling rhamnose fermentation in FIRN and non-FIRN rha− strains of Salmonella typhimurium: an essay in bacterial archaeology

Published online by Cambridge University Press:  14 April 2009

A. Morgenroth  and
J. P. Duguid
A. Morgenroth
Affiliation:
Bacteriology Department, University of Dundee
J. P. Duguid
Affiliation:
Bacteriology Department, University of Dundee
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Observations of mutation and phage-mediated transduction of rhamnose-fermentation properties were made on two groups of wild-type rhamnose-non-fermenting (rha−) strains of Salmonella typhimurium isolated in several different countries. The ‘FIRN’ group included twenty-three strains in the biotypes 15, 16, 17 and 18, and the ‘non-FIRN rha−’ group included fourteen strains in a new biotype, provisionally designated 19 X d. The two groups showed different patterns of mutational behaviour and the pattern was uniform for the different strains in each group. Non-FIRN rha− strains gave rise directly to colonies of rha+ mutant bacteria in platings on rhamnose minimal media. FIRN strains were unable to do this, but produced rha+ bacteria by two successive mutations, the first of which yielded weakly fermenting (rhaw) bacteria capable of fermenting rhamnose in peptone water, but not of utilizing it for growth on minimal medium.

Rha+ recombinant bacteria were produced in transductional crosses between any one FIRN strain and any one non-FIRN rha− strain, but not in any cross between one FIRN strain and another FIRN strain or between one non-FIRN rha− strain and another non-FIRN rha− strain. These findings suggest that although the sites of the rha− mutations are different between the FIRN and non-FIRN rha− groups, they are located at identical sites on the chromosome in all strains in either group. It is concluded that all FIRN strains have probably descended from a single ancestral strain which underwent mutations at two sites in the rhamnose region of the chromosome and that all non-FIRN rha− strains have descended from a single ancestral bacterium which underwent a mutation at a third site in the rhamnose region.

Type
Research Article
Information
Genetics Research , Volume 11 , Issue 2 , April 1968 , pp. 151 - 169
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Boyd, J. S. K. (1956). Immunity of lysogenic bacteria. Nature, Lond. 178, 141.CrossRefGoogle ScholarPubMed
Brinton, C. C. Jr, Buzzell, A. & Lauffer, M. A. (1954). Electrophoresis and phage susceptibility studies on a filament producing variant of the E. coli B bacterium. Biochim. biophys. Acta 15, 533–542.CrossRefGoogle Scholar
Callow, Bessie R. (1959). A new phage-typing scheme for Salmonella typhimurium. J. Hyg., Camb. 57, 346–359.CrossRefGoogle Scholar
Davis, B. D. & Mingioli, E. S. (1950). Mutants of Escherichia coli requiring methionin or vitamin B12. J. Bact. 60, 17–28.CrossRefGoogle ScholarPubMed
Demerec, M., Moser, H., Hemmerly, J., Blomstrand, I., Demerec, Z. E., Fitzgerald, P. L., Glover, S. W., Hanson, J. F., Nielsen, F. J. & Yura, T. (1954). Bacterial genetics, I. Yb. Carnegie Instn. Wash. 53, 225–241.Google Scholar
Duguid, J. P., Anderson, E. S. & Campbell, I. (1966). Fimbriae and adhesive properties in salmonellae. J. Path. Bact. 91, 107–138.CrossRefGoogle Scholar
Duguid, J. P., Old, D. C. & Hume, V. B. (1962). Transduction of fimbriation and rhamnose fermentation characters in Salmonella typhimurium. Proc. Genet. Soc. Edinb. p. 138.Google Scholar
Duguid, J. P., Smith, I. W., Dempster, G. & Edmunds, P. N. (1955). Non-flagellar filamentous appendages (‘fimbriae’) and haemagglutinating activity in Bacterium coli. J. Path. Bact. 70, 335–348.CrossRefGoogle ScholarPubMed
Harhoff, N. (1948). Gastroenteritisbaciller af Salmonellagruppen i Danmark. Nyt nordisk Forlag, pp. 23, 42. Copenhagen.Google Scholar
Hartman, P. E. (1956). Genetic studies with bacteria. Pubis. Carnegie Instn. no. 612, pp. 35–62.Google Scholar
Houwink, A. L. & Van Iterson, W. (1950). Electron-microscopical observations on bacterial cytology. II. Study on flagellation. Biochim. biophys. Acta 5, 10–44.CrossRefGoogle Scholar
Kallings, L. O. & Laurell, A. B. (1957). Relation between phage types and fermentation types of Salmonella typhimurium. Acta path. microbiol. scand. 40, 328–342.Google Scholar
Kauffmann, F. (1954). Enterobacteriaceae, 2nd ed., p. 42. Copenhagen: Ejnar Munksgaard.Google Scholar
Kristensen, M., Bojlén, K. & Faarup, C. (1937). Bacteriologisk-epidemiologiske Erfaringer om Infektioner med Gastroenteritisbaciller af Paratyfusgruppen. Bibltk Laeger 129, 310–375.Google Scholar
Old, D. C. (1963). Fimbriation in Enterobacteriaceae. Ph.D. thesis, University of Edinburgh.Google Scholar
Power, J. (1967). The L-rhamnose genetic system in Escherichia coli K-12. Genetics 55, 557–568.CrossRefGoogle ScholarPubMed
Sanderson, K. E. & Demerec, M. (1965). The linkage map of Salmonella typhimurium. Genetics 51, 897–913.CrossRefGoogle ScholarPubMed
Stocker, B. A. D. & Edgar, J. B. (1959). Annual Report of the Board of Governors of the Lister Inst. p. 13. Also Proc. Path. Soc. Gt Brit. Ireland, 8 January 1959 (unpublished).Google Scholar
Subbaiah, T. V. & Stocker, B. A. D. (1962). Mapping of the correlated characters fimbriation and rhamnose-fermentation in Salmonella typhimurium using col-agents as fertility factors. Proc. Genet. Soc. Edinb. pp. 138.Google Scholar
Swanstrom, M. & Adams, M. H. (1951). Agar layer method for production of high titer phage stocks. Proc. Soc. exp. Biol. Med. 78, 372–375.CrossRefGoogle ScholarPubMed
Zinder, N. D. & Lederberg, J. (1952). Genetic exchange in Salmonella. J. Bact. 64, 679–699.CrossRefGoogle ScholarPubMed