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Cytogenetic manifestations associated with the reversion, by gene amplification, at the HGPRT locus in V79 Chinese hamster cells

Published online by Cambridge University Press:  14 April 2009

A. Di Leonardo ,
C. Agnese ,
P. Cavolina ,
A. Maddalena ,
G. Sciandrello  and
R. Randazzo
A. Di Leonardo
Affiliation:
Department of Cell and Developmental Biology ‘A. Monroy’, University of Palermo, via Archiraft 22, 90123 Palermo, Italy
C. Agnese
Affiliation:
Department of Cell and Developmental Biology ‘A. Monroy’, University of Palermo, via Archiraft 22, 90123 Palermo, Italy
P. Cavolina
Affiliation:
Department of Cell and Developmental Biology ‘A. Monroy’, University of Palermo, via Archiraft 22, 90123 Palermo, Italy
A. Maddalena
Affiliation:
Department of Cell and Developmental Biology ‘A. Monroy’, University of Palermo, via Archiraft 22, 90123 Palermo, Italy
G. Sciandrello
Affiliation:
Department of Cell and Developmental Biology ‘A. Monroy’, University of Palermo, via Archiraft 22, 90123 Palermo, Italy
R. Randazzo
Affiliation:
Department of Cell and Developmental Biology ‘A. Monroy’, University of Palermo, via Archiraft 22, 90123 Palermo, Italy
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Summary

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Some HGPRT spontaneous revertants were isolated from a mutant line (E2) of V79 Chinese hamster cells and phenotypically characterized. Dot–Blot hybridization with a 32P-Iabelled HGPRT probe revealed an increase in the number of HGPRT sequences in some of these revertants, suggesting the occurrence of gene amplification. Cytogenetic analysis performed in three of these revertants showed a characteristic abnormally banding region (ABR) on the elongated p arm of the X chromosome. In Situ hybridization in one revertant (RHE2) showed that the amplified sequences reside on the p+ arm of the X chromsome in two different localizations. Because of the very probable clonal origin of the revertant, these features indicate that the amplified sequences might rearrange after their integration into the chromosome.

Type
Research Article
Information
Genetics Research , Volume 53 , Issue 3 , June 1989 , pp. 201 - 206
Copyright
Copyright © Cambridge University Press 1989

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