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Cytogenetic and complementation analyses of recessive lethal mutations induced in the X chromosome of Drosophila by three alkylating agents

Published online by Cambridge University Press:  14 April 2009

J. K. Lim  and
L. A. Snyder
J. K. Lim
Affiliation:
Department of Biology, University of Wisconsin-Eau Claire, Eau Claire, Wisconsin 54701
L. A. Snyder
Affiliation:
Department of Genetics and Cell Biology, University of Minnesota, St Paul, Minnesota 55108
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Salivary-gland chromosomes of 54 methyl methanesulphonate- and 50 triethylene melamine-induced X-chromosome recessive lethals in Drosophila melanogaster were analysed. Two of the lethals induced by the mono-functional agent and 11 of those induced by the polyfunctional agent were found to be associated with detectable aberrations. A complementation analysis was also done on 82 ethyl methanesulphonate- and 34 triethylene melamine-induced recessive lethals in the zeste-white region of the X chromosome. The EMS-induced lethals were found to represent lesions affecting only single cistrons. Each of the 14 cistrons in the region known to mutate to a lethal state was represented by mutant alleles, but in widely different frequencies. Seven of the TEM-induced lethals were associated with deletions, only one of which had both breakpoints within the mapped region. Twenty-six of the 27 mutations in which only single cistrons were affected were mapped to 7 of the 14 known loci. One TEM- and two EMS-induced mutations were alleles representing a previously undetected locus in the zeste-white region.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 1 , August 1974 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 1974

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