Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T13:17:00.407Z Has data issue: false hasContentIssue false
  • English
  • Français

Cytogenetic and complementation analyses of recessive lethal mutations induced in the X chromosome of Drosophila by three alkylating agents

Published online by Cambridge University Press:  14 April 2009

J. K. Lim  and
L. A. Snyder
J. K. Lim
Affiliation:
Department of Biology, University of Wisconsin-Eau Claire, Eau Claire, Wisconsin 54701
L. A. Snyder
Affiliation:
Department of Genetics and Cell Biology, University of Minnesota, St Paul, Minnesota 55108
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Salivary-gland chromosomes of 54 methyl methanesulphonate- and 50 triethylene melamine-induced X-chromosome recessive lethals in Drosophila melanogaster were analysed. Two of the lethals induced by the mono-functional agent and 11 of those induced by the polyfunctional agent were found to be associated with detectable aberrations. A complementation analysis was also done on 82 ethyl methanesulphonate- and 34 triethylene melamine-induced recessive lethals in the zeste-white region of the X chromosome. The EMS-induced lethals were found to represent lesions affecting only single cistrons. Each of the 14 cistrons in the region known to mutate to a lethal state was represented by mutant alleles, but in widely different frequencies. Seven of the TEM-induced lethals were associated with deletions, only one of which had both breakpoints within the mapped region. Twenty-six of the 27 mutations in which only single cistrons were affected were mapped to 7 of the 14 known loci. One TEM- and two EMS-induced mutations were alleles representing a previously undetected locus in the zeste-white region.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 1 , August 1974 , pp. 1 - 10
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Auerbach, C. & Kilbey, B. J. (1971). Mutation in eukaryotes. Annual Review of Genetics 5, 163–218.Google Scholar
Beermann, W. (1972). Chromomeres and genes. In Developmental Studies on Giant Chromosomes (ed. Beermann, W.), pp. 1–33. New York, Heidelberg: Springer-Verlag.CrossRefGoogle Scholar
Bishop, J. B. & Lee, W. R. (1969). Ethyl methanesulfonate (EMS) induced mutations in Drosophila melanogaster. Genetics 61, s4–5.Google Scholar
Bishop, J. B. & Lee, W. R. (1973). Chromosome breakage in Drosophila melanogaster induced by a monofunctional alkylating agent (EMS). Mutation Research 21, 327–333.CrossRefGoogle ScholarPubMed
Bridges, C. B. (1938). A revised map of the salivary gland X chromosome of Drosophila melanogaster. Journal of Heredity 29, 11–13.CrossRefGoogle Scholar
Fahmy, O. G. & Fahmy, M. J. (1955). Cytogenetic analysis of the action of carcinogens and tumour inhibitors in Drosophila melanogaster. IV. The cell stage during spermatogenesis and the induction if intra- and inter-genic mutations by 2:4:6-tri(ethyleneimino)-1:3:5-triazine. Journal of Genetics 53, 563–584.Google Scholar
Fahmy, O. G. & Fahmy, M. J. (1956). Cytogenetic analysis of the action of carcinogens and tumour inhibitors in Drosophila melanogaster. V. Differential genetic response to the alkylating mutagens and X-radiation. Journal of Genetics 54, 146–164.CrossRefGoogle Scholar
Fahmy, O. G. & Fahmy, M. J. (1959). Drosophila melanogaster; new mutants. Drosophila Information Service 33, 82–94.Google Scholar
Fahmy, O. G. & Fahmy, M. J. (1961). Cytogenetic analysis of the action of carcinogens and tumour inhibitors in Drosophila melanogaster. X. The nature of the mutations induced by the mesyloxy esters in relation to molecular cross-linkage. Genetics 46, 447–458.CrossRefGoogle Scholar
Hochman, B. (1971). Analysis of chromosome 4 in Drosophila melanogaster. II. Ethyl methanesulfonate-induced lethals. Genetics 67, 235–252.CrossRefGoogle ScholarPubMed
Judd, B. H., Shen, M. W. & Kaufman, T. C. (1972). The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. Genetics 71, 139–156.CrossRefGoogle ScholarPubMed
Lawley, P. D. (1961). The action of alkylating agents on deoxyribonucleic acid. Journal de Ghimie Physique 58, 1011–1020.Google Scholar
Lewis, E. B. & Bacher, F. (1968). Method of feeding ethyl methane sulfonate (EMS) to Drosophila males. Drosophila Information Service 43, 193.Google Scholar
Lifschytz, E. & Falk, R. (1969). Fine structure analysis of a chromosome segment in Drosophila melanogaster. Analysis of ethyl methanesulfonate-induced lethals. Mutation Research 8, 147–155.CrossRefGoogle Scholar
Lim, J. K. & Snyder, L. A. (1968). The mutagenic effects of two monofunctional alkylating chemicals on mature spermatozoa of Drosophila. Mutation Research 6, 129–137.Google Scholar
Lindsley, D. L. & Grell, E. H. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institution of Washington, publication no. 627.Google Scholar
Muller, H. J. (1940). An analysis of the process of structural change in chromosomes of Drosophila. Journal of Genetics 40, 1–66.CrossRefGoogle Scholar
Rudkin, G. T. (1965). The relative mutabilities of DNA in regions of the X chromosome of Drosophila melanogaster. Genetics 52, 665–681.CrossRefGoogle ScholarPubMed
Schalet, A. (1972). Lethal, semilethal and male sterile mutants in the proximal region of the X chromosome of D. melanogaster. Drosophila Information Service 49, 64–66.Google Scholar
Slizynska, H. & Slizynski, B. M. (1947). Genetical and cytological studies of lethals induced by chemical treatment in Drosophila melanogaster. Proceedings of the Royal Society of Edinburgh B 62, 234–242.Google ScholarPubMed
Williamson, J. H. (1970). Ethyl methanesulfonate-induced mutants in the Y chromosome of Drosophila melanogaster. Mutation Research 10, 597–605.CrossRefGoogle Scholar