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Contribution of recombinants produced by female flies heterozygous for Est-α alleles to genie variation of Drosophila virilis

Published online by Cambridge University Press:  14 April 2009

Kendo Tsuno
Kendo Tsuno
Affiliation:
Department of Biology, Josai Dental University, Sakado, Sailama 350–02, Japan
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With 12 alleles at the Est-α locus of Drosophila virilis, 44 genotypes of females heterozygous for a pair of them were constructed, and about 4 × 104 of their F1 progenies per genotype were electrophoretically examined. Eighty-three variants different from both of their parental alleles were obtained from a total of 2·0 × 106 progenies. Statistical analysis suggested most of their variants to be recombinants. And no null or inactive allele, of which frequency is several percent in natural populations, appeared in 11·6 × 104 progenies from females heterozygous for active alleles. These results suggest that the high variation of the Est-α locus in this species is generated by recombinants from female heterozygotes.

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 3 , June 1988 , pp. 217 - 222
Copyright
Copyright © Cambridge University Press 1988

References

Ayala, F. J., Tracey, M. L., Barr, L. G., McDonald, J. F. & Perez-Saras, S. (1974). Genetic variation in natural populations of five Drosophila species and the hypothesis of the selective neutrality of protein polymorphisms. Genetics 77, 343–384.CrossRefGoogle ScholarPubMed
Chino, M. (1936). The genetics of Drosophila virilis. Japanese Journal of Genetics 12, 189–210 (in Japanese).Google Scholar
Coyne, J. A. & Felton, A. A. (1977). Genetic heterozygosity at two alcohol dehydrogenase loci in Drosophila pseudoobscura and Drosophila persimilis. Genetics 87, 285–304.CrossRefGoogle Scholar
Ebitani, N. (1976). Comparison of fitness among genotypes in Drosophila virilis with the different level of esterase activity. Japanese Journal of Genetics 51, 1–10.Google Scholar
Fisher, R. A. & Yates, F. (1963). Statistical Tables, 6th edn.Longman Group.Google Scholar
Fristrom, J. W. & Yund, M. A. (1973). Genetic programming for development in Drosophila. Critical Reviews of Biochemistry 1, 537–570.CrossRefGoogle ScholarPubMed
Fukatami, A. (1977). Enzyme polymorphism in natural populations of Drosophila lutescens. Japanese Journal of Genetics 52 (2), 341–356.Google Scholar
Kreitman, M. (1983). Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster. Nature 304, 412–417.CrossRefGoogle ScholarPubMed
Laird, C. D. (1973). DNA of Drosophila chromosomes. Annual Review of Genetics 7, 177–204.CrossRefGoogle ScholarPubMed
Lefevre, G. Jr, (1971). Salivary chromosome bands and the frequency of crossing over in Drosophila melanogaster. Genetics 67, 497–513.CrossRefGoogle ScholarPubMed
Mukai, T. & Cockerham, C. C. (1977). Spontaneous mutation rates at enzyme loci in Drosophila melanogaster. Proceedings of the National Academy of Sciences, USA 74, 2514–2517.CrossRefGoogle ScholarPubMed
Prakash, S. (1974). Gene differences between the sex ratio and standard gene arrangements of the X chromosome and linkage disequilibrium between loci in the standard gene arrangement of the X chromosome in Drosophila pseudoobscura. Genetics 77, 795–804.CrossRefGoogle ScholarPubMed
Rasch, E. M., Barr, H. J. & Rasch, R. W. (1971). The DNA content of sperm of Drosophila melanogaster. Chromosoma 33, 1–18.CrossRefGoogle ScholarPubMed
Sasaki, M. & Narise, S. (1978). Molecular weight of esterase isozymes of Drosophila species. Drosophila Information Service 53, 123.Google Scholar
Singh, R. S., Lewontin, R. C. & Felton, A. A. (1976). Genetic heterogeneity within electrophoretic ‘alleles’ of xanthine dehydrogenase in Drosophila pseudoobscura. Genetics 84, 609–629.CrossRefGoogle ScholarPubMed
Thatcher, D. R. (1980). The complete amino acid sequence of three alcohol dehydrogenase alleloenzyme (AdhN − 11, Adhs and AdhUF) from the fruitfly Drosophila melanogaster. Biochemical Journal 187, 875–886.CrossRefGoogle ScholarPubMed
Thuriaux, P. (1977). Is recombination confined to structural genes on the eukaryotic genome? Nature 268, 460–462.CrossRefGoogle ScholarPubMed
Tsuno, K. (1975). Esterase gene frequency differences and linkage equilibrium in Drosophila virilis populations from different ecological habitats. Genetics 80, 585–594.CrossRefGoogle ScholarPubMed
Tsuno, K. (1981). Studies on mutation at esterase loci in Drosophila virilis. I. Spontaneous mutation rates and newly arisen variants. Japanese Journal of Genetics 56, 155–174.Google Scholar
Tsuno, K. (1982). Studies on mutation at esterase loci in Drosophila virilis. II. Intragenic recombination at the Est-α locus. Japanese Journal of Genetics 57, 259–267.Google Scholar
Tsuno, K. (1985). Studies on mutation at esterase loci in Drosophila virilis. III. Genie variation in Est-α alleles produced by mutation experiments. Japanese Journal of Genetics 60, 103–118.Google Scholar
Tsuno, K., Aotsuka, N. T. & Ohba, S. (1984). Further genetic variation at the esterase loci of Drosophila virilis. Biochemical Genetics 22 (3/4), 323–337.CrossRefGoogle ScholarPubMed
Voelker, R. A., Langley, C. H., Brown, A. J. L., Ohnishi, S., Bickson, B., Montgomery, E. & Smith, S. (1980). Enzyme null alleles in natural populations of Drosophila melanogaster. Genetics 70, 397–403.Google Scholar