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A comparative study of the coats of chimaeric mice and those of heterozygotes for X-linked genes

Published online by Cambridge University Press:  14 April 2009

B. M. Cattanach ,
H. G. Wolfe  and
M. F. Lyon
B. M. Cattanach
Affiliation:
M.R.C. Radiobiology Unit, Harwell, Didcot, Berks
H. G. Wolfe
Affiliation:
M.R.C. Radiobiology Unit, Harwell, Didcot, Berks
M. F. Lyon
Affiliation:
M.R.C. Radiobiology Unit, Harwell, Didcot, Berks
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The coats of Ta–Ta+ and c–c+ chimaeric mice have been compared with those of Ta heterozygotes and animals heterozygous for an X-autosome translocation which causes c-variegation. With one exception all those features typical of the heterozygotes were found in their chimaeric counterparts, thus showing that the creation of two cell populations by X-inactivation can ultimately lead to the observed heterozygous phenotypes. The one exceptional feature not found in c-variegated chimaeric mice was the progessive increase in the proportion of pigmented hairs in c areas with age which occurs in X-autosome translocation heterozygotes.

The coat of a Modp / + – Ta chimaeric animal was also investigated. Ta hairs showing only the colour effect of Modp were numerous, suggesting that Modp and Ta affect different cell populations. However, Ta hairs showing the structural effect of Modp were very rare. The two observations suggest Modp can operate independently upon two different cell types – melanocytes and hair follicle cells.

Type
Research Article
Information
Genetics Research , Volume 19 , Issue 3 , June 1972 , pp. 213 - 228
Copyright
Copyright © Cambridge University Press 1972

References

REFERENCES

Biggers, J. D., Whitten, W. K. & Whittingham, D. G. (1971). The culture of mouse embryos in vitro. In Methods in Mammalian Embryology, ed. Daniel, J. C. Jr. San Francisco: W. H. Freeman and Co.Google Scholar
Cattanach, B. M. (1961). A chemically-induced variegated-type position effect in the mouse. Zeitschrift für Vererbungslehre 92, 165–182.Google ScholarPubMed
Cattanach, B. M. & Isaacson, J. H. (1965). Genetic control over the inactivation of autosomal genes attached to the X-chromosome. Zeitschrift für Vererbungslehre 96, 313–323.Google ScholarPubMed
Danes, B. S. & Bearn, A. C. (1967). Hurler's syndrome: a genetic study of clones in cell culture with particular reference to the Lyon hypothesis. Journal of Experimental Medicine 126, 509–522.CrossRefGoogle Scholar
Davidson, R. G., Nitowsky, H. M. & Childs, B. (1963). Demonstration of two populations of cells in the human female heterozygous for glucose-6-phosphate dehydrogenase variants. Proceedings of the National Academy of Sciences 50, 481–485.CrossRefGoogle ScholarPubMed
Evans, E. P., Breckon, G. & Ford, C. E. (1964). An air-drying method for meiotic preparations from mammalian testes. Cytogenetics 3, 289–294.CrossRefGoogle ScholarPubMed
Ford, C. E. & Hamerton, J. L. (1956). A colchicine, hypotonie citrate, squash sequence for mammalian chromosomes. Stain Technology 31, 247–251.CrossRefGoogle Scholar
Fredga, K. (1964). A simple technique for demonstration of the chromosomes and mitotic stages in a mammal. Chromosomes from cornea. Hereditas 51, 268–273.CrossRefGoogle Scholar
Gartler, S. M., Gandini, E., Hutchison, H. T., Campbell, B. & Zechhi, G. (1971 a). Glucose-6-phosphate dehydrogenase mosaicism: utilisation in the study of hair follicle variegation. Annals of Human Genetics 35 (1), 1–7.CrossRefGoogle Scholar
Gartler, S. M., Scott, R. C., Goldstein, J. L. & Campbell, B. (1971 b). Lesch-Nyhan syndrome: Rapid detection of heterozygotes by use of hair follicles. Science 172, 572–574.CrossRefGoogle ScholarPubMed
Goldstein, J. L., Marks, J. F. & Gartler, S. M. (1971). Expression of two X-linked genes in human hair follicles of double heterozygotes. Proceedings of the National Academy of Sciences 68, 1425–1427.CrossRefGoogle ScholarPubMed
Gruneberg, H. (1966). More about the tabby mouse and about the Lyon hypothesis. Journal of Embryology and Experimental Morphology 16, 569–590.Google ScholarPubMed
Gruneberg, H. (1969). Threshold phenomena versus cell heredity in the manifestation of sex-linked genes in mammals. Journal of Embryology and Experimental Morphology 22, 145–179.Google ScholarPubMed
Lyon, M. F. (1961). Gene action in the X-chromosome of the mouse (Mus musculus L.). Nature 190, 372–373.CrossRefGoogle ScholarPubMed
Lyon, M. F. (1963). Attempts to test the inactive-X theory of dosage compensation in mammals. Genetical Research 4, 93–103.CrossRefGoogle Scholar
Lyon, M. F. (1968). Chromosomal and subchromosomal inactivation. Annual Review of Genetics 2, 31–52.CrossRefGoogle Scholar
Lyon, M. F. & Meredith, R. (1966). Autosomal translocations causing male sterility and viable aneuploidy in the mouse. Cytogenetics 5, 335–354.CrossRefGoogle ScholarPubMed
Migeon, B. R., der Kalaustian, V. M., Nyhan, W. L., Young, W. J. & Childs, B. (1968). X-linked hypoxanthine-guanine phosphoribosyl transferase deficiency: Heterozygote has two clonal populations. Science 160, 425–427.CrossRefGoogle ScholarPubMed
Mintz, B. (1962). Experimental study of the developing mammalian egg: removal of the zona pellucida. Science 138, 594–595.CrossRefGoogle ScholarPubMed
Mintz, B. (1964). Formation of genetically mosaic mouse embryos and early development of ‘lethal (t 12/t 12)-normal’ mosaics. Journal of Experimental Zoology 157, 273–292.CrossRefGoogle ScholarPubMed
Mintz, B. (1967 a). Mammalian embryo culture. From Methods in Developmental Biology, ed. Wilt, F. H. and Wessells, N. K.. New York: T. Y. Crowell Co.Google Scholar
Mintz, B. (1967 b). Gene control of mammalian pigmentary differentiation. I. Clonal origin of melanocytes. Proceedings of the National Academy of Sciences 58, 344–351.CrossRefGoogle ScholarPubMed
Mintz, B. (1968). Hermaphroditism, sex chromosomal mosaicism and germ cell selection in allophenic mice. Journal of Animal Science (Suppl. 1), 27, 51–60.Google ScholarPubMed
Mintz, B. (1969). Developmental mechanisms found in allophenic mice with sex chromosomal and pigmentary mosaicism. Birth Defects: Original Article Series, vol. V, no. 1, pp. 11–22.Google Scholar
Mintz, B. (1971). Clonal basis of mammalian differentiation. In Control Mechanisms of Growth and Differentiation. Symposia of the Society for Experimental Biology, no. xxv, pp. 345–370.Google Scholar
Mintz, B. & Palm, J. (1969). Gene control of hematopoiesis. I. Erythrocyte mosaicism and permanent immunological tolerance in allophenic mice. Journal of Experimental Medicine 129, 1013–1027.CrossRefGoogle ScholarPubMed
Mystkowska, E. T. & Tarkowski, A. K. (1968). Observations in CBA-p/CBA-T6T6 mouse chimaeras. Journal of Embryological and Experimental Morphology 20, 33–52.Google Scholar
Nesbitt, M. N. (1971). X chromosome inactivation mosaicism in the mouse. Developmental Biology 26, 252–263.CrossRefGoogle Scholar
Romeo, G. & Migeon, B. R. (1970). Genetic inactivation of the α-galactocidase locus in carrier of Fabry's disease. Science 170, 180–181.CrossRefGoogle ScholarPubMed
Tarkowski, A. K. (1961). Mouse chimaeras developed from fused eggs. Nature, London 190, 857–860.CrossRefGoogle ScholarPubMed
Wegmann, T. G. & Gilman, J. G. (1970). Chimaerism for three genetic systems in tetraparental mice. Developmental Biology 21, 281–291.CrossRefGoogle Scholar
Stevens, L. C. & Hummel, K. P. (1957). A description of spontaneous congenital testicular teratomas in strain 129 mice. Journal of the National Cancer Institute 18, 719–748.Google ScholarPubMed