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Chromosomal location of a mutation causing chloramphenicol resistance in Escherichia coli K 12

Published online by Cambridge University Press:  14 April 2009

E. C. R. Reeve  and
D. R. Suttie
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh 9
D. R. Suttie
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh 9
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A chloramphenicol-resistant mutation in Escherichia coli K 12, cmlA1 (previously designated 1a), giving a higher Cm-resistance than other mutations yet examined, has been shown to have a chromosomal location, the gene order being gal, λ, bio, cmlA, pyrD. CmlA can be transduced efficiently into cm-sensitive strains by P1 with little phenotypic lag, and is co-transduced with the λ-attachment site (frequency 1·13%) but not with gal or pyrD.

Type
Research Article
Information
Genetics Research , Volume 11 , Issue 1 , February 1968 , pp. 97 - 104
Copyright
Copyright © Cambridge University Press 1968

References

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers.Google Scholar
Anderson, E. S. & Lewis, M. J. (1965 a). Drug resistance and its transfer in Salmonella typhimurium. Nature, Lond. 206, 579–583.Google Scholar
Anderson, E. S. & Lewis, M. J. (1965 b). Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature, Lond. 208, 843–849.Google Scholar
Broda, P. (1967). The formation of Hfr strains in Escherichia coli K 12. Genet. Res. 9, 35–48.Google Scholar
Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76.Google Scholar
Lennox, E. S. (1955). Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206.Google Scholar
Reeve, E. C. R. & Bishop, J. O. (1965). Variations in resistance to three antibiotics among some single-step mutants to chloramphenicol resistance in a strain of Escherichica coli K 12. Genet. Res. 6, 310–315.Google Scholar
Reeve, E. C. R. (1966). Characteristics of some single step mutants to chloramphenicol resistance in Escherichia coli K 12 and their interactions with R-factor genes. Genet. Res. 7, 281–286.CrossRefGoogle Scholar
Rothman, June L. (1965). Transduction studies on the relation between prophage and host chromosome. J. molec. Biol. 12, 892–912.Google Scholar
Watanabe, T. (1963). Infective heredity of multiple drug resistance in bacteria. Bact. Rev. 27, 87–114.Google Scholar