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Chromosomal location of a barley malt endopeptidase gene

Published online by Cambridge University Press:  14 April 2009

J. R. Guerin ,
R. C. M. Lance  and
W. Wallace
J. R. Guerin
Affiliation:
Department of Plant Science Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia, 5064
R. C. M. Lance
Affiliation:
Department of Plant Science Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia, 5064
W. Wallace
Affiliation:
Department of Plant Science Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia, 5064
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Extracts of disomic wheat-barley addition lines were tested for the presence of a barley malt endopeptidase (MEP-1) by employing isoelectric focusing (IEF) and western blotting. The blots were probed with polyclonal antibodies raised against MEP-1 purified from the endosperms of 5-day-old germinated barley seedlings. The endopeptidase was detected in the Betzes barley cultivar and the addition line containing the full genome of the wheat cultivar Chinese Spring plus a chromosome 3 pair from Betzes barley. The endopeptidase was not expressed in Chinese Spring nor the addition lines containing other Betzes chromosome pairs. The endopeptidase was detected in a ditelosomic addition line containing the long arm of Betzes chromosome 3. We have concluded that the gene coding for MEP-1 (Cep-B) is located on the long arm of Betzes chromosome 3.

Type
Research Article
Information
Genetics Research , Volume 59 , Issue 3 , June 1992 , pp. 179 - 181
Copyright
Copyright © Cambridge University Press 1992

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