Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-23T10:25:26.419Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of the F transfer cistron, traL

Published online by Cambridge University Press:  14 April 2009

N. S. Willetts
N. S. Willetts
Affiliation:
M.R.C. Molecular Genetics Unit, Department of Molecular Biology, University of Edinburgh
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The properties of two transfer-deficient Flac mutants (in Escherichia coli K12) carrying mutations in the cistron traL are described. The traL product is needed for conjugational DNA transfer and for pilus formation, but not for surface exclusion; it is not plasmid-specific. traL maps between traA and traE; one of the traL mutations is apparently polar on traA. The properties of Tra+ revertants of this mutation suggest that the traL product is directly required for F-specific phage infection.

Type
Research Article
Information
Genetics Research , Volume 21 , Issue 2 , April 1973 , pp. 205 - 213
Copyright
Copyright © Cambridge University Press 1973

References

REFERENCES

Achtman, M., Willetts, N. S. & Clark, A. J. (1971). Beginning a genetic analysis of conjugational transfer determined by the F factor in E. coli by isolation and characterization of transfer-deficient mutants. Journal of Bacteriology 106, 529–538.CrossRefGoogle Scholar
Achtman, M., Willetts, N. S. & Clark, A. J. (1972). Conjugational complementation analysis of transfer-deficient mutants of Flac in E. coli. Journal of Bacteriology 110, 831–842.CrossRefGoogle Scholar
Alfaro, G. & Willetts, N. S. (1972). The relationship between the transfer systems of some bacterial plasmids. Genetical Research 20.Google ScholarPubMed
Finnegan, D. J. & Willetts, N. S. (1971). Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Molecular and General Genetics 111, 256–264.CrossRefGoogle ScholarPubMed
Ippen-Ihler, K., Achtman, M. & Willetts, (1972). Deletion map of the E. coli sex factor F: the order of eleven transfer genes. Journal of Bacteriology 110, 857–863.CrossRefGoogle Scholar
Model, P., Webster, R. E. & Zinder, N. D. (1969). The UGA codon in vitro: chain termination and suppression. Journal of Molecular Biology 43, 177–190.CrossRefGoogle Scholar
Ohtsubo, E. (1970). Transfer-defective mutants of sex-factors in E. coli. II. Deletion mutants of an F prime and deletion mapping of cistrons involved in genetic transfer. Genetics 64, 189–197.CrossRefGoogle Scholar
Ohtsubo, E., Nishimura, Y. & Hirota, Y. (1970). Transfer-defective mutants of sex factors in E. coli. I. Defective mutants and complementation analysis. Genetics 64, 173–188.CrossRefGoogle Scholar
Willetts, N. S. (1970). The interaction of an I-like R factor and transfer-deficient mutants of Flac in E. coli K12. Molecular and General Genetics, 108, 365–373.CrossRefGoogle Scholar
Willetts, N. S. (1971). Plasmid specificity of two proteins required for conjugation in E. coli K12. Nature New Biology 230, 183–185.CrossRefGoogle ScholarPubMed