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Characterization of a new choline locus in Aspergillus nidulans and its significance for choline metabolism

Published online by Cambridge University Press:  14 April 2009

Paul Markham
Affiliation:
Department of Microbiology, Queen Elizabeth College, Campden Hill Road, London W8 7AH
Brian W. Bainbridge
Affiliation:
Department of Microbiology, Queen Elizabeth College, Campden Hill Road, London W8 7AH
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Summary

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A new locus for choline-requirement has been identified in Aspergillus nidulans and designated choC3. It has been located to linkage group VIII at a position 31·9 ± 2·2 map units to the left of the sD locus.

It is proposed that this locus codes either for the enzyme that catalyses the transmethylation of phosphatidyl monomethylaminoethanol to phosphatidyl dimethylaminoethanol or for a molecule essential for the synthesis or function of this enzyme.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Arst, H. N. (1968). Genetic analysis of the first steps of sulphate metabolism in Aspergillus nidulans. Nature 219, 268270.CrossRefGoogle ScholarPubMed
Arst, H. N. (1971). Mutants of Aspergillus nidulans unable to use choline-O-sulphate. Genetical Research 17, 273277.CrossRefGoogle Scholar
Bremer, J. & Greenberg, D. M. (1959). Mono- and dimethylethanolamine isolated from rat-liver phospholipids. Biochimica et biophysica acta 35, 287288.CrossRefGoogle ScholarPubMed
Bremer, J. & Greenberg, D. M. (1960). Biosynthesis of choline in vitro. Biochimica et biophysica acta 37, 173175.CrossRefGoogle ScholarPubMed
Bremer, J. & Greenberg, D. M. (1961). Methyl transferring enzyme system of microsomes in the biosynthesis of lecithin (phosphatidylcholine). Biochimica et biophysica acta 46, 205216.CrossRefGoogle Scholar
Bremer, J., Figard, P. H. & Greenberg, D. M. (1960). The biosynthesis of choline and its relation to phospholipid metabolism. Biochimica et biophysica acta 43, 477488.CrossRefGoogle Scholar
Clutterbuck, A. J. (1973). Gene symbols in Aspergillus nidulans. Genetical Research 21, 291296.CrossRefGoogle ScholarPubMed
Clutterbuck, A. J. (1974). Aspergillus nidulans. In Handbook of Genetics, vol. 1 (ed. King, R. C.). PP. 447510. New York and London: Plenum Press.Google Scholar
Crocken, B. J. & Nyc, J. F. (1964). Phospholipid variations in mutant strains of Neurospora crassa. Journal of Biological Chemistry 239, 17271730.CrossRefGoogle ScholarPubMed
Gravel, R. A. (1976). Choline-O-sulphate utilization in Aspergillus nidulans. Genetical Research 28, 261276.CrossRefGoogle ScholarPubMed
Horowitz, N. H. (1946). The isolation and identification of a natural precursor of choline. Journal of Biological Chemistry 162, 413419.CrossRefGoogle ScholarPubMed
Horowitz, N. H., Bonner, D. & Houlahan, M. B. (1945). The utilization of choline analogues by cholineless mutants of Neurospora. Journal of Biological Chemistry 159, 145151.CrossRefGoogle Scholar
Käfer, E. (1958). An eight-chromosome map of Aspergillus nidulans. Advances in Genetics 9, 105145.CrossRefGoogle Scholar
Kaneshiro, T. & Law, J. H. (1964). Phosphatidyl choline synthesis in Agrobacterium tumefaciens. 1. Purification and properties of a phosphatidylethanolamine N-methyltransferase. Journal of Biological Chemistry 239, 17051713.CrossRefGoogle Scholar
Kennedy, E. P. & Weiss, S. B. (1956). The function of cytidine coenzymes in the biosynthesis of phospholipides. Journal of Biological Chemistry 222, 193214.CrossRefGoogle ScholarPubMed
Kubitschek, H. E. (1969). Counting and sizing microorganisms with the Coulter counter. In Methods in Microbiology, vol. 1 (ed. Norris, J. R. and Ribbons, D. W.), pp. 593610. London: Academic Press.Google Scholar
Mackintosh, M. E. & Pritchard, R. H. (1963). Production and replica plating of microcolonies of Aspergillus nidulans. Genetical Research 4, 320322.CrossRefGoogle Scholar
Markham, P. & Bainbridge, B. W. (1978). A morphological lesion (ballooning) related to a requirement for choline in mutants of Aspergillus nidulans. Proceedings of the Society for General Microbiology 5, 65.Google Scholar
Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D. & Bufton, A. W. J. (1953). The genetics of Aspergillus nidulans. Advances in Genetics 5, 141238.CrossRefGoogle ScholarPubMed
Rehbinder, D. & Greenberg, D. M. (1965). Studies on the methylation of ethanolamine phosphatides by liver preparations. Archives of Biochemistry and Biophysics 109, 110115.CrossRefGoogle ScholarPubMed
Roberts, C. F. (1959). A replica plating technique for the isolation of nutritionally exacting mutants of a filamentous fungus (Aspergillus nidulans). Journal of General Microbiology 20, 540548.CrossRefGoogle ScholarPubMed
Scarborough, G. A. & Nyc, J. F. (1967 a). Methylation of ethanolamine phosphatides by microsomes from normal and mutant strains of Neurospora crassa. Journal of Biological Chemistry 242, 238242.CrossRefGoogle ScholarPubMed
Scarborough, G. A. & Nyc, J. F. (1967 B). Properties of a phosphatidyl-monomethylethanolamine N-methyltransferase from Neurospora crassa. Biochimica et biophysica acta 146, 111119.CrossRefGoogle Scholar
Singh, M. & Sinha, U. (1976). Chloral hydrate induced haploidization in Aspergillus nidulans. Experientia 32, 11441145.CrossRefGoogle ScholarPubMed
Steiner, M. R. & Lester, R. L. (1970). In vitro study of the methylation pathway of phosphatidylcholine synthesis and the regulation of this pathway in Saccharomyces cerevisiae. Biochemistry 9, 6369.CrossRefGoogle ScholarPubMed
Valentine, B. P. (1975). The isolation and characterisation of temperature sensitive mutants of Aspergillus nidulans, with special reference to cell wall synthesis and mannose utilisation. Ph.D. Thesis, University of London.Google Scholar
Waldron, C. & Roberts, C. F. (1974). Cold-sensitive mutants in Aspergillus nidulans. I. Isolation and general characterisation. Molecular and General Genetics 134, 99113.CrossRefGoogle ScholarPubMed