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Cellular damage associated with multiple mating in E. coli

Published online by Cambridge University Press:  14 April 2009

J. D. Gross
J. D. Gross
Affiliation:
Medical Research Council Microbial Genetics Research Unit, Hammersmith Hospital, London, W.12
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In crosses in which the ratio of Hfr to F- cells was varied it was found that with one group of Hfr strains the yield of recombinants increased linearly with donor concentration until the number of donor and recipient cells were approximately equal. With other Hfr strains marked deviations from linearity were observed at Hfr: F− ratios greater than 1:5 and in crosses with one particular F− strain the yield of recombinants actually decreased progressively as the Hfr concentration was raised beyond this value. The deviations from linearity observed with this group of strains have been shown to be due to damage to F− cells which mate simultaneously with several Hfr cells.

Type
Research Article
Information
Genetics Research , Volume 4 , Issue 3 , November 1963 , pp. 463 - 469
Copyright
Copyright © Cambridge University Press 1963

References

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