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cDNA sequence and chromosomal localization of the mouse parvalbumin gene, Pva

Published online by Cambridge University Press:  14 April 2009

Ch. Zühlke ,
F. Schöffl ,
H. Jockusch ,
D. Simon  and
J.-L. Guénet
Ch. Zühlke
Affiliation:
Genetics Unit, University of Bielefeld, D-4800 Bielefeld 1, FRG
F. Schöffl*
Affiliation:
Genetics Unit, University of Bielefeld, D-4800 Bielefeld 1, FRG
H. Jockusch
Affiliation:
Developmental Biology Unit, University of Bielefeld, D-4800 Bielefeld 1, FRG
D. Simon
Affiliation:
Unité de Génétique des Mammifères, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France
J.-L. Guénet
Affiliation:
Unité de Génétique des Mammifères, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France
*
* To whom correspondence should be addressed.
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In the homozygous condition, the mutation adr (arrested development of righting response) of the mouse causes a myotonia and a drastic reduction of the Ca2+-binding protein parvalbumin (PV) in fast muscles. Using a rat PV probe, a mouse cDNA clone was isolated from a λgt11 wild-type fast-muscle library and its nucleotide sequence was determined. The protein coding and the 3′ non-translated regions of the mouse gene show extensive homology with the rat PV gene. The result of Southern blot hybridization is consistent with a single copy gene for parvalbumin. Restriction fragment length polymorphisms (RFLPs) between Mus musculus domesticus (e.g. C57BL/6) and Mus spretus (SPE) were detected with the enzymes Eco RI, Pst I, and Sst I. The restriction fragment patterns of DNA samples from 65 individual offspring of (C57BL/6 × SPE)F1 × C57BL/6 backcrosses were tested with the PV probe and matched, for linkage detection, to pre-existing patterns established with various RFLP probes on the same samples. A co-distribution of PV-RFLPs with Pvt-1 and Mlvi-2, which had been localized on chromosome 15, was detected. Thus, the structural gene for PV, designated Pva, maps to chromosome 15 of the mouse whereas the adr mutation shows no linkage with markers on this chromosome. Gene locus homology between chromsome 15 of the mouse and chromosome 22 of man (which carries the human PV gene) is discussed.

Type
Research Article
Information
Genetics Research , Volume 54 , Issue 1 , August 1989 , pp. 37 - 44
Copyright
Copyright © Cambridge University Press 1989

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