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The bar-properties, in particular glucosylation of deoxy-ribonucleic acid, in crosses of bacteriophages T2 and T4*

Published online by Cambridge University Press:  14 April 2009

B. de Groot
Affiliation:
Department of Radiation Genetics, University of Leiden, and Institute for Radiopathology and Radiation Protection, The Netherlands
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Analysis of the inheritance of the three bar-properties of bacteriophage T4: exclusion of T2 from the progeny of crosses, glucosylation of the hydroxymethylcytosine (HMC)moiety of the DNA according to T4, and plating with large plaques on E. coli K strains, was carried out by means of marker rescue from T4 by T2 on E. coli K (λh) as a selective indicator. Five of the strains isolated plated with large plaques on K (λh), but did not exclude T2 and showed T2 glucosylation; plating on E. coli K (λh) was found to segregate from the other two bar-properties. The sixth isolate showed, in addition to plating with large plaques on K, partial non-excludability by the parental T4 and T4 glucosylation of HMC. If partial non-excludability is the result of T4 glucosylation, the role of the additional glucose substitutions might be a protective effect on the DNA against the exclusion factor of T4. This proposal is supported by the analysis of the progeny from a single burst from a cross of T4 and T2. The following T2 genes were partially excluded: host-range, no exclusion of parental T2, sensitivity to ultraviolet, and limited plating efficiency on E. coli K (λh). The exclusion factor of T4 is not transmitted to all progeny and does not behave like a bar-property. Only resistance to exclusion and T4 glucosylation were transmitted to all twenty-seven progeny of the single burst. The elimination of sensitivity to exclusion and T2 glucosylation is explained by assuming that the recombinant class with the exclusion factor of T4 and T2 α-glucosylation will exclude itself and be suicidal upon infection of a new host. Exclusion and differential glucosylation are discussed with regard to restriction and modification, respectively.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1967

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publ.CrossRefGoogle Scholar
Arber, W. (1965). Host specificity of DNA produced by Escherichia coli. V. The role of methionine in the production of host specificity. lJ. molec. Biol. 11, 247256.CrossRefGoogle ScholarPubMed
Edgar, R. S. & Epstein, R. H. (1963). Conditional lethal mutations in bacteriophage T4. ‘Genetics Today’. Proc. XI. Int. Congr. Genet. (Geerts, S. J., ed.), 2, 116. The Hague.Google Scholar
de Groot, B. (1966 a). Marker rescue of the adsorption properties of bacteriophage T2. Antonie van Leeuwenhoek, 32, 1724.CrossRefGoogle ScholarPubMed
de Groot, B. (1966 b). Partial exclusion or bacteriophage T2 by T4: An early function in the second linkage group. Genetica, 37, 3751.CrossRefGoogle ScholarPubMed
Hershey, A. D. & Rotman, R. (1949). Genetic recombination between host range and plaque-type mutants of bacteriophage in single bacterial cells. Genetics, 34, 4471.CrossRefGoogle ScholarPubMed
Jesaitis, M. A. (1961). The inheritance of the glucose component of the phage nucleic acids. J. gen. Physiol. 44, 585609.CrossRefGoogle ScholarPubMed
Kornberg, S. R., Zimmerman, S. B. & Kornberg, A. (1961). Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli. J. biol. Chem. 236, 14871493.CrossRefGoogle ScholarPubMed
Lehman, I. R. & Pratt, E. A. (1960). On the structure of the glucosylated hydroxymethyl- cytosine nucleotides of coliphages T2, T4 and T6. J. biol. Chem. 235, 32543259.CrossRefGoogle Scholar
Pratt, E. A., Kuno, S. & Lehman, I. R. (1963). Glucosylation of the deoxyribonucleic acid in hybrids of coliphages T2 and T4. Biochim. biophys. Acta, 68, 108111.CrossRefGoogle ScholarPubMed
Richardson, C. C. (1966). Influence of glucosylation of deoxyribonucleic acid on hydrolysis by deoxyribonucleases of Escherichia coli. J. biol. Chem. 241, 20842092.CrossRefGoogle ScholarPubMed
Shedlovsky, A. & Brenner, S. (1963). A chemical basis for the host-induced modification of T-even bacteriophages. Proc. natn. Acad. Sci. U.S.A. 50, 300305.CrossRefGoogle ScholarPubMed
Stahl, F. W., Edgar, R. S. & Steinberg, J. (1964). The linkage map of bacteriophage T4. Genetics, 50, 539552.CrossRefGoogle ScholarPubMed
Streisinger, G. (1956 a). The genetic control of ultraviolet sensitivity levels in bacteriophages T2 and T4. Virology, 2, 112.CrossRefGoogle ScholarPubMed
Streisinger, G. (1956 b). The genetic control of host range and serological specificity in bacteriophages T2 and T4. Virology, 2, 377387.CrossRefGoogle ScholarPubMed
Streisingr, G. & Weigle, J. (1956). Properties of bacteriophages T2 and T4 with unusual inheritance. Proc. natn. Acad. Sci. U.S.A. 42, 504510.CrossRefGoogle Scholar
de Waard, A. (1964). On the specificity of bacteriophage induced hydroxymethylcytosine glucosyltransferases. I. Specificity differences between the hydroxymethylcytosine— glucosyltransferases induced by bacteriophages T2, T4 and T6. Biochim. biophys. Acta, 92 286304.Google ScholarPubMed