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Antisuppressor mutations in Aspergillus nidulans: cold-resistant revertants of suppressor suaC109

Published online by Cambridge University Press:  14 April 2009

S. D. Martinelli
S. D. Martinelli
Affiliation:
Department of Biology, Birkbeck College (University of London), Malet Street London WC1 7HX
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Cold-resistant revertants of the cold-sensitive, ribosomal suppressor suaC109 have been isolated, with a view to obtaining mutations in new ribosomal protein genes. Many revertants had reduced suppressor activity and were classified as antisuppressor mutants. Both intragenic and extragenic reversions were found. In seven strains the extragenic reversion to cold resistance segregated with the antisuppressor phenotype, and these were designated asu mutations. Three of the five asu genes, C, B and D were mapped to linkage groups, I, II and V respectively. The antisuppressors are not gene-specific, although they mainly antagonize the activity of ribosomal suppressors. The antisuppressors altered all aspects of the phenotype of suppressor suaC109 including sensitivity to aminoglycoside antibiotics, and are therefore thought to be mutations in ribosomal protein genes.

Type
Research Article
Information
Genetics Research , Volume 49 , Issue 3 , June 1987 , pp. 191 - 200
Copyright
Copyright © Cambridge University Press 1987

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