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Analysis of a rare recombination event within the multigenic Hor 2 locus of barley (Hordeum vulgare L.)

Published online by Cambridge University Press:  14 April 2009

Peter R. Shewry ,
Saroj Parmar ,
Julian Franklin  and
Shirley R. Burgess
Peter R. Shewry*
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Saroj Parmar
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Julian Franklin
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
Shirley R. Burgess
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
*
* Professor P. R. Shewry, Department of Agricultural Sciences, University of Bristol, AFRC Institute of Arable Crops Research, Long Ashton Research Station, Bristol, BS18 9AF, U.K.
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A rare recombinant within the multigenic Hor 2 locus of barley was detected by SD-PAGE of hordein fractions from F2 grain from the cross Bomi × P12/3. Analysis of a homozygous F4 line by 2-D IEF/SDS-PAGE showed that recombination between the class I/II and class III subfamilies of genes had occurred, indicating that they are spatially separate within the Hor 2 locus. RFLP analysis using a B hordein-related cDNA clone confirmed that recombination had occurred, while similar analysis using a genomic clone related to γ-type hordein (encoded by the Hor 5/HrdF locus) indicated the order of the two subfamilies of genes on the short arm of chromosome 5, the class I/II genes being closer to the centromere. The results are consistent with the origin of the B hordein gene family from initial duplication of a single ancestral gene to give two genes which diverged to become the ancestors of the class I/II and class III subfamilies. Subsequent cycles of duplication and divergence have resulted in the present high degree of polymorphism.

Type
Research Article
Information
Genetics Research , Volume 55 , Issue 3 , June 1990 , pp. 171 - 176
Copyright
Copyright © Cambridge University Press 1990

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