Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-26T15:11:33.720Z Has data issue: false hasContentIssue false
  • English
  • Français

An investigation into the origins of meiotic aneuploidy using ascus analysis

Published online by Cambridge University Press:  14 April 2009

A. M. Fulton  and
D. J. Bond
A. M. Fulton
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN, Scotland
D. J. Bond
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh EH9 3JN, Scotland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Aneuploidy can result from a variety of defects at meiosis. Results are presented of crosses of Sordaria brevicollis in which aneuploid spores are detected through complementation of spore colour mutants at the buff or grey-6 loci on linkage groups II and IV respectively. By using ascus analysis, the underlying cause of the aneuploidy can be deduced in many cases. Thus non-conjuction (pairing failure) and non-disjunction at the first meiotic division, premature centromere division, non-disjunction at the second division, and pre-meiotic errors such as extra replication of the chromosomes can be distinguished. Both linkage groups were found to give a similar proportion of the different errors. Non-conjunction and first-division non-disjunction formed 60–70% of detectable cases, whilst premature centromere division and second-division non-disjunction comprised 10% and 5% of aneuploids respectively. However, only a small proportion of second-division errors are detected.

It is proposed that the systems described in this paper can form the basis of a valuable screening method for detecting agents which increase aneuploid frequency. The advantages and disadvantages of using lower eukaryotes in this way are discussed.

Type
Research Article
Information
Genetics Research , Volume 41 , Issue 2 , April 1983 , pp. 165 - 179
Copyright
Copyright © Cambridge University Press 1983

References

REFERENCES

Alberman, E. D. & Creasy, M. R. (1977). Frequency of chromosomal abnormalities in miscarriages and perinatal deaths. Journal of Medical Genetics 14, 313–315.CrossRefGoogle ScholarPubMed
Beechey, C. V. (1973). X-Y chromosome dissociation and sterility in the mouse. Cytogenetics and Cell Genetics 12, 60–67.CrossRefGoogle ScholarPubMed
Berg, C. M. (1966). Biased distribution and polarized segregation in asci of Sordaria brevicollis. Genetics 53, 117–129.CrossRefGoogle ScholarPubMed
Bond, D. J. (1976). A system for the study of meiotic non-disjunction using Sordaria brevicollis. Mutation Research 37, 213–220.CrossRefGoogle Scholar
Bond, D. J. (1982). Systems for detecting meiotic aneuploidy in Sordaria and Neurospora. In Handbook of Mutagenicity Test Procedures, 2nd ed. (eds Kilbey, B. J., Legator, M., Nicholls, W. and Ramel, C.).Google Scholar
Bond, D. J. & McMillan, L. (1979). Meiotic aneuploidy: its origins and induction following chemical treatment in Sordaria brevicollis. Environmental Health Perspectives 31, 67–74.CrossRefGoogle ScholarPubMed
Boué, J., Boué, A. & Lazar, P. (1975). Retrospective and prospective epidemiological studies of 1500 karyotyped spontaneous human abortions. Teratology 12, 11–26.CrossRefGoogle ScholarPubMed
Bryan, J. (1972). Definition of three classes of binding site in isolated microtubule crystals. Biochemistry 11, 2611–2616.CrossRefGoogle ScholarPubMed
Chandley, A. C., MacLean, N., Edmond, P., Fletcher, J. & Watson, G. S. (1976). Cytogenetics and infertility in man. II. Testicular histology and meisosis. Annals of human Genetics 40, 165–176.CrossRefGoogle Scholar
Chen, K. C. & Olive, L. S. (1965). The genetics of Sordaria brevicollis. II. Biased segregation due to spindle overlap. Genetics 51, 761–766.CrossRefGoogle ScholarPubMed
Davis, B. K. (1971). Genetic analysis of a meiotic mutant resulting in precocious sister centromere separation in Drosophila melanogaster. Molecular General Genetics 113, 251–272.CrossRefGoogle ScholarPubMed
Fitzgerald, P. H., Pickering, A. F., Mercer, J. M. & Miethke, P. M. (1975). Premature centromere division: a mechanism of non-disjunction causing X-chromosome aneuploidy in somatic cells of Man. Annals of Human Genetics 38, 417–428.CrossRefGoogle ScholarPubMed
Galloway, S. M. & Buckton, K. E. (1978). Aneuploidy and ageing: chromosome studies on a random sample of the population using G-banding. Cytogenetics and Cell Genetics 20, 78–95.CrossRefGoogle ScholarPubMed
German, J. (1979). Roberts' syndrome: 1. Cytological evidence for a disturbance in chromatid pairing. Clinical Genetics 16, 441–447.CrossRefGoogle Scholar
Golubovskaya, I. N. (1979). Genetic control of meiosis. International Review of Cytology 58, 247–290.CrossRefGoogle ScholarPubMed
Haber, J. E., Peloquin, J. G., Halvorson, H. O. & Borisy, G. G. (1972). Colcemid inhibition of cell growth and the characterization of a colcemid-binding activity in Saccharomyces cerevisiae. Journal of Cell Biology 55, 355–367.CrossRefGoogle ScholarPubMed
Hansmann, I. & El-Nahass, E. (1979). Incidence of non-disjunction in mouse oocytes. Cytogenetics and Cell Genetics 24, 115–121.CrossRefGoogle Scholar
Heath, I. B. (1975). The effect of antimicrotubule agents on the growth and ultrastructure of the fungus Saprolegnia ferax and their ineffectiveness in disrupting hyphal microtubules. Protoplasma 85, 147–176.CrossRefGoogle ScholarPubMed
Jacobs, P. A. & Morton, N. M. (1977). Origin of human trisomics and polyploids. Human Heredity 27, 59–72.CrossRefGoogle ScholarPubMed
Langenbeck, U., Hansmann, I., Hinney, B. & Honig, U. (1976). On the origin of the supernumerary chromosome in autosomal trisomics with special reference to Down's Syndrome. A bias in tracing non-disjunction by chromosomal and biochemical polymorphisms. Human Genetics 33, 89–102.CrossRefGoogle Scholar
Merriam, J. R. & Frost, J. N. (1964). Exchange and non-disjunction of the X chromosome in female Drosophila melanogaster. Genetics 49, 109–122.CrossRefGoogle Scholar
Moustacchi, E., Hottinguer De Margerie, H. & Fabre, F. (1967). A novel character induced in yeast by P32 decay: the ability to manifest high frequencies of abnormal meiotic segregations. Genetics 57, 909–918.CrossRefGoogle ScholarPubMed
Polani, P. E. & Jagiello, G. M. (1976). Chiasmata, meiotic univalents and age in relation to aneuploid imbalance in mice. Cytogenetics and Cell Genetics 16, 505–529.CrossRefGoogle ScholarPubMed
Purnell, D. J. (1973). Spontaneous univalence at male meiosis in the mouse. Cytogenetics and Cell Genetics 12, 327–335.CrossRefGoogle ScholarPubMed
Sansome, E. R. & Bannon, L. (1946). Colchicine ineffective in inducing polyploidy in Penicillium notatum. Lancet ii, 828–829.CrossRefGoogle Scholar
Speed, R. M. (1977). The effects of ageing on the meiotic chromosomes of male and female mice. Chromosoma 64, 241–254.CrossRefGoogle ScholarPubMed
Sturtevant, A. H. & Beadle, G. W. (1939). An Introduction to Genetics. W. B. Saunders.Google Scholar
Sugawara, S. & Mikamo, K. (1980). An experimental approach to the analysis of mechanisms of meiotic non-disjunction and anaphase lagging in primary oocytes. Cytogenetics and Cell Genetics 28, 251–264.CrossRefGoogle Scholar
Threlkeld, S. G. H. & Stoltz, J. M. (1970). A genetic analysis of non-disjunction and mitotic recombination in Neurospora crassa. Genetical Research 16, 29–35.CrossRefGoogle ScholarPubMed
Vogel, H. J. (1956). A convenient growth medium for Neurospora (medium N). Microbial Genetics Bulletin 13, 42–43.Google Scholar