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Absence of clustering of functionally related genes in Pseudomonas aeruginosa*

Published online by Cambridge University Press:  14 April 2009

Barbara Fargie  and
B. W. Holloway
Barbara Fargie
Affiliation:
Department of Bacteriology, University of Melbourne, Melbourne, Victoria, Australia
B. W. Holloway
Affiliation:
Department of Bacteriology, University of Melbourne, Melbourne, Victoria, Australia
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Phage F116 transduces sufficiently large fragments of the Pseudomonas aeruginosa chromosome for co-transduction of linked markers to occur. The linkage relationships of 176 auxotrophic mutants have been examined by means of this technique, following characterization of the mutants for their nutritional requirements and by other biochemical means. Thirty-two different loci from fourteen biosynthetic pathways have been differentiated by such combined means. Unlike other bacteria, the clustering of related loci of a biosynthetic pathway appears to be rare. This situation was confirmed for the leucine, methionine, adenine, isoleucine plus valine and arginine pathways. No evidence for linkage of markers within the histidine, proline, cysteine and uracil pathways has been found but further data is needed to completely confirm this conclusion for these groups of markers. The importance of these findings in relation to the genetic control of enzyme biosynthesis in P. aeruginosa is discussed. For markers of the tryptophan pathway, two are possibly linked but the other three are definitely not linked. The only confirmed linkage of related markers was between a homoserine locus and a threonine locus.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 2 , July 1965 , pp. 284 - 299
Copyright
Copyright © Cambridge University Press 1965

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