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Transferability of SSR markers from lychee (Litchi chinensis Sonn.) to pulasan (Nephelium ramboutan-ake L.)

Published online by Cambridge University Press:  08 February 2006

Chun Hock Sim ,
Mansor Clyde Mahani ,
Chee Yen Choong  and
Idris Salma
Chun Hock Sim
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Mansor Clyde Mahani
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Chee Yen Choong
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Idris Salma
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
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Abstract

Introduction. Pulasan and lychee are from the same family and closely related, therefore the SSR markers are expected to be highly transferable between these two taxa. We investigated the transferability of 12 lychee (Litchi chinensis Sonn.) simple sequence repeat (SSR) loci to pulasan (Nephelium ramboutan-ake L.). Materials and methods. Genomic DNA was extracted from 20 accessions of pulasan for the PCR amplification of the SSR loci using 12 pairs of SSR primers derived from lychee. The PCR products were resolved on denaturing polyacrylamide gels. Results. The percentage of SSR transferability from lychee to pulasan was 58.3% and the percentage of polymorphic SSR markers was 25%. Discussion. The moderate transferability and low polymorphism rates suggest the possibility of interruptions within the repeat motif and mutations in the flanking sequences of SSR repeat motifs. Our results did not reveal a high rate of transferability between lychee and pulasan. However, this study showed that the SSR markers developed in lychee are a good source of molecular markers for pulasan.

Keywords

MalaysiaLitchi chinensisNephelium ramboutan-akemolecular biologygenetic markersPCRgenetic polymorphismMalaisieLitchi chinensisNephelium ramboutan-akebiologie moléculairemarqueur génétiquePCRpolymorphisme génétique
Type
Research Article
Information
Fruits , Volume 60 , Issue 6 , November 2005 , pp. 379 - 385
Copyright
© CIRAD, EDP Sciences, 2005

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