Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-22T19:58:52.943Z Has data issue: false hasContentIssue false
  • English
  • Français

Propagation of citrus somatic hybrids with potential for utilization as rootstocks

Published online by Cambridge University Press:  15 April 2006

Rafael Pio ,
Francisco de Assis Alves MourãoFilho ,
Beatriz Madalena Januzzi Mendes ,
Fábio Albuquerque Entelmann  and
André Siqueira Rodrigues Alves
Rafael Pio
Affiliation:
Instituto Agronômico (IAC), Centro Avançado de Pesquisa Tecnológica do Agronegócio de Frutas, Av. Luiz Pereira dos Santos, nº 1500, Corrupira, 13214-820, Jundiaí, SP, Brazil
Francisco de Assis Alves MourãoFilho
Affiliation:
Departamento de Produção Vegetal, USP/ESALQ, CP 9, Piracicaba, SP, 13418-900, Brazil
Beatriz Madalena Januzzi Mendes
Affiliation:
Laboratório de Biotecnologia Vegetal, USP/CENA, CP 96, Piracicaba, SP, 13400-970, Brazil
Fábio Albuquerque Entelmann
Affiliation:
Departamento de Produção Vegetal, USP/ESALQ, CP 9, Piracicaba, SP, 13418-900, Brazil
André Siqueira Rodrigues Alves
Affiliation:
Departamento de Produção Vegetal, USP/ESALQ, CP 9, Piracicaba, SP, 13418-900, Brazil
Get access

Abstract

Introduction. Alternative propagation techniques must be developed in order to reduce the time required to evaluate germplasm in citrus breeding programs. Our study investigated the rooting capacity and initial plant development of different citrus somatic hybrids that have potential for use as rootstocks. Materials and methods. Eleven different hybrids were tested. Cuttings of these plants were placed in trays inside an intermittent mist chamber. After 100 days, the percentage of cuttings with callous and with roots, average number of roots, and root length were recorded. The cuttings were then transferred to plastic bags and kept in the greenhouse. The flush length was recorded monthly. After 7 months, the percentage of plants suitable for grafting and the average scion and root dry mass were calculated. Results and discussion. [Caipira sweet orange + Cleopatra mandarin], [Caipira sweet orange + Volkamer lemon], [Rangpur lime + Sunki mandarin] and [Rohde Red Valencia sweet orange + Volkamer lemon] hybrids developed many roots, while [Caipira sweet orange + Volkamer lemon] and [Rohde Red Valencia sweet orange + Volkamer lemon] hybrids showed the best development after the transplant.

Keywords

BrazilCitrus sp.protoplast fusioncuttingsrootingBrésilCitrus sp.fusion de protoplastesboutureenracinement
Type
Research Article
Information
Fruits , Volume 61 , Issue 1 , January 2006 , pp. 1 - 7
Copyright
© CIRAD, EDP Sciences, 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Grosser, J.W., Ollitrault, P., Olivarer-Fuster, O., Somatic hybridization in Citrus: an effective tool to facilitate variety improvement, In Vitro Cell. Dev.-Pl. 36 (2000) 434–449. CrossRef
Grosser, J.W., Gmitter, F.G. Jr., Protoplast fusion and citrus improvement, Plant Breeding Rev. 8 (1990) 339–374.
Platt R.G., Optiz K.W., Propagation of Citrus, in: Reuther W. (Eds.), The citrus industry, Univ. Calif. Press, Berkeley, USA, 1973, p. 1–47.
Boava, L.P., Siviero, A., Masuda, Y., Furtado, E.L., Machado, M.A., Resistência de citrandarins e citranges a Phytophthora parasitica, Laranja 24 (1) (2003) 135–144.
Frost H.B., Soost R.K., Seed reproduction: development of gametes and embryos, in: Reuther W., Batchelor L.D., Webber H.J. (Eds.), The citrus industry, Univ. Calif. Press, Berkeley, USA, 1968, p. 290–324.
Soost R.K., Cameron J.W., Citrus, in: Janick J., Moore J.N. (Eds.), Advances in fruit breeding, Purdue Univ. Press, West Lafayette, USA, 1975, p. 507–540.
Costa, M.A.P.C., Mendes, B.M.J., Mourão Filho, F.A.A., Somatic hybridization for improvement of citrus rootstock: production of five new combinations with potential for improved disease resistance, Aust. J. Exp. Agr. 43 (2003) 1151–1156. CrossRef
Mendes, B.M.J., Mourão Filho, F.A.A., Farias, P.C.M., Benedito, V.A., Citrus somatic hybridization with potential for improved blight and CTV resistance, In Vitro Cell. Dev.-Pl. 37 (2001) 490–495. CrossRef
Mendes-da-Glória, F.J., Mourão Filho, F.A.A., Camargo, L.E.A., Mendes, B.M.J., Caipira sweet orange + Rangpur lime: a potential somatic hybrid for use as roostock in the Brazilian citrus industry, Genet. Mol. Biol. 23 (3) (2000) 661–665. CrossRef
Fachinello J.C., Hoffmann A., Nachtigal J.C., Kersten E., Fortes G.R.L., Propagação de plantas frutíferas de clima temperado, UFPel, Pelotas, Brazil, 1995, 178 p.
Pasqual M., Chalfun N.N.J., Ramos J.D., Vale M.R., Silva, C.R.R., Fruticultura comercial: Propagação de plantas frutíferas, UFLA/FAEPE, Lavras, Brazil, 2001, 137 p.
Ollitrault, P., Dambier, D., Froelicher, Y., Bakry, F., Aubert, B., Rootstock breeding strategies for the Mediterranean citrus industry: the somatic hybridization potential, Fruits 53 (5) (1998) 335–344.
Sabbah, S.M., Grosser, J.W., Chandler, J.L., Louzada, E.S., The effect of growth regulators on the rooting of stem cultures of Citrus, related genera and intergeneric somatic hybrids, Proc. Fla. State Hortic. Soc. 104 (1992) 188–191.
Andrade, R.A., Martins, A.B.G., Propagação vegetativa de porta-enxertos para citros, Rev. Bras. Frutic. 25 (1) (2003) 134–136. CrossRef
Pio, R., Ramos, J.D., Gontijo, T.C.A., Carrijo, E.P., Coelho, J.H.C., Alvares, B.F., Mendonça, V., Enraizamento de estacas dos porta-enxertos de citros ‘Fly Dragon’ e ‘Trifoliata’, Rev. Bras. Agrociênc. 8 (3) (2002) 195–198.
Santos R.F.A., Villas Boas R.M.F., Salibe A.A., Estudos sobre o enraizamento de estacas de citros com aplicação de agentes de efeito hormonal, in: IX Cong. Bras. Frutic., Campinas, Brazil, 1987, p. 387–393.
Pratti, P., Mourão Filho, F.A.A., Dias, C.T.S., Scarpare Filho, J.A., Estaquia semi-lenhosa: um método rápido e alternativo para a produção de mudas de lima ácida ‘Tahiti’, Sci. Agric. 56 (1) (1999) 185–190. CrossRef
Rocha, A.C., Tavares, E.D., Sandrini, M., Carvalho, S.A., Silva, L.F.C., Propagação de três espécies de citros através do enraizamento de estacas verdes, Rev. Bras. Frutic. 10 (2) (1988) 31–33.
Janick J.A., A ciência da horticultura, Freitas Bastos, Rio de Janeiro, Brazil, 1966, 485 p.
Hartmann H.T., Kester D.E., Davies F.T. Jr., Geneve R.L., Plant propagation: principles and practices, 7 ed., Prentice Hall, New Jersey, USA, 2002, 880 p.