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Meristem culture of two fig cultivars in Turkey

Published online by Cambridge University Press:  07 March 2007

Songul Comlekcioglu ,
Ayzin B. Kuden ,
Yldz Aka Kacar  and
Muharrem A. Kamberoglu
Songul Comlekcioglu
Affiliation:
 Horticultural Department, Agricultural Faculty, Cukurova University, 01330 Adana, Turkey
Ayzin B. Kuden
Affiliation:
 Horticultural Department, Agricultural Faculty, Cukurova University, 01330 Adana, Turkey
Yldz Aka Kacar
Affiliation:
 Horticultural Department, Agricultural Faculty, Cukurova University, 01330 Adana, Turkey
Muharrem A. Kamberoglu
Affiliation:
 Plant Protection Department, Agricultural Faculty, Cukurova University, 01330 Adana, Turkey
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Abstract

Introduction. Two Turkish fig cultivars, Alkuden and Bursa Siyahi, were propagated by meristem culture to eliminate the fig mosaic virus. The technique of dsRNA analysis was conducted on the in vitro propagated plants to test for virus-free status. Materials and methods. Four different Murashige and Skoog (MS) media complementedwith different concentrations of growth hormones (GA3, BA and IBA) were compared to study the cultured meristems’ survival rate, shoot formation and rooting. Short- and long-term thermotherapy treatments were also applied. Results. For survival rate, 0.2 mg GA3·L–1 + 0.5 mg BA·L–1 gave the most favorable results; for shoot formation, it was the medium with 0.2 mg GA3·L–1 + 2.0 mg BA·L–1 which was the best, while rooting rate was the highest for meristems cultured on MS medium with only 0.1 mg GA3·L–1. Conclusion. Meristem culture, together with thermotherapy treatment, is recommended to obtain virus-free fig plant material. Although cultured plants seemed to be very healthy, dsRNA virus tests are recommended for sensitive evaluation of the sanitary status of the plants obtained.

Keywords

TurkeyFicus caricamicropropagationmeristem cultureplant growth substancesheat therapyplant virusesTurquieFicus caricamicropropagationculture de méristèmesubstance de croissance végétalethermothérapievirus des végétaux
Type
Research Article
Information
Fruits , Volume 62 , Issue 2 , March 2007 , pp. 125 - 131
Copyright
© CIRAD, EDP Sciences, 2007

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References

Özbek, S., ncir yetistiriciligi, Çözel meyvecilik, ÇÜZF, Yayn No 128, Ders Kitab 11 (1978) 392–483 (in Turkish).
Anon., Fig production, FAO, Rome, Italy, 2005.
Gella, R., Marin, J.A., Corrales, M. L., Toribio, F., Elimination of fig mosaic from fig shoot-tip cultures by thermotherapy, in: Proc. 1st Int. Symp. fig, zmir,Turkey, 24–28 June 1997, Acta Hortic. 480 (1998) 173–177. CrossRef
Açkgöz S., DökmenT.M., Incir mozaik hastaligi etmeninin tantlmasnda dsrna analiz yönteminin kullanlmas, Türkiye IX, Fitopatol. Kongr., Trakya Üniv. Rektörlügü Yaynlar, No. 45, Tekirda, Turkey, 2001 (in Turkish).
Demiralay, A., Mendi-Yalçn, Y., Aka-Kaçar, Y., Çetiner, S., In vitro propagation of Ficus carica L. var. Bursa Siyah through meristem culture. in: Proc. 1st Int. Symp. fig, zmir, Turkey, 24–28 June 1997, Acta Hortic. 480 (1998) 165–168. CrossRef
Günver, G., Ertan, E., A study on the propagation of figs by tissue culture techniques, in: Proc. 1st Int. Symp. Fig, zmir, Turkey, 24–28 June 1997, Acta Hortic. 480 (1998) 169–172. CrossRef
Nobr, J., Romano, A., In vitro cloning of Ficus carica L. adult trees, in: Proc. 1st Int. Symp. Fig, zmir, Turkey, 24–28 June 1997, Acta Hortic. 480 (1998) 161–164. CrossRef
Hepaksoy S., Aksoy U., Propagation of Ficus carica L. clones by in vitro culture, Biol. Plant. 50 (3) (2006) 433–436.
Robert U., Zel J., Ravnikar M., Thermotherapy in virus elimination from garlic: influences on shoot multiplication from meristems and bulb formation in vitro, Sci. Hortic. 73 (4) (1998) 193–202.
Verma, N., Ram, R., Hallan, V., Kumar, K., Zaidi, A.A., Production of cucumber mosaic virus-free chrysanthemums by meristem tip culture, Crop Prot. 23 (2004) 469–473. CrossRef
Katoh, N., Yui, M., Sato, S., Shirai, T., Yuasa, H., Hagimori, M., Production of virus-free plants from virus-infected sweet pepper by in vitro grafting, Sci. Hortic. 100 (2004) 1–6. CrossRef
Truskinov E.V., Rogozina E.V., Elimination of viruses from a potato clone collection by tissue culture, Russ. J. Plant Physiol. 44 (3) (1997) 374–380.
Manganaris G.A., Economou A.S., Boubourakas I.N., Katis N.I., Elimination of PPV and PNRSV through thermotherapy and meristem-tip culture in nectarine, Plant Cell Rep. 22 (3) (2003) 195–200.
Ram R., Verma N., Singh A.K., Singh L., Hallan V., Zaidi A.A., Indexing and production of virus-free chrysanthemums, Biol. Plant. 49 (1) (2005) 149–152.
Kaka N., Kuden A.B., Kuden A., Çetiner S., Ege bölgesi incirleri ile çukurova bölgesinden selekte edilen incirlerin adana’ya adaptasyonu üzerine çalismalar, ÇÜZF, Derg. 5 (4) (1990) 77–86 [in Turkish].
Kaka N., Kuden A.B., Cebeci E., Türkiye 1, ulusal bahçe bitkileri kongresi, Cilt 1 (Meyve), Ege Üniv., Ziraat Fak., Bornova, zmir, Turkey, 1992 [in Turkish].
Kabasakal A., ncir yetistiriciligi, TAV Yaynlar, No. 20, 96 s., Yalova, Turkey, 1990 (in Turkish).
Kuden A.B., Tanrver E.,. Baz incir çeitlerinin meyvelerini olgunlatrabilmek için gereksinim duyduklar “büyüme derece saatleri toplam” (bdst) üzerine aratrmalar, Türkiye II, Ulusal bahçe bitkileri kongresi, Cilt I (Meyve), ÇÜZF, Bahçe Bitkileri Bölümü, Adana, Turkey, 775 (1995) 668–671 [in Turkish].
Kuden A.B., Plant genetic resources of fig in Turkey, 3rd, Gen. l Meet. Mesfin, Adana, Turkey, 1999, 105–112.
Kuden A.B., Güneydogu anadolu bölgesinde incir yetistiriciligi, 2006, (in press).
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plantarum 15 (1962) 473–497. CrossRef
Barbosa, W., Campo-Doll’orto F.A., Ojma M., Martines F.P., Bovi V., Castro J.L., In vitro production of fig tree cuttings from cv. Roxo de Valinhas, Agron 44 (1–3) (1992) 6–10.
Kumar V., Rodha A., Chitta S.K., In vitro plant regeneration of fig (Ficus carica l., cv. Gular) apical buds from mature trees, Plant Cell Rep. 17 (9) (1998) 717–720.
Brum G.R., Silva A.B., Posqual M., Effect of different concentrations of BAP and NAA in the fig (Ficus carica L.) in vitro propagation, Cienc. Agrotec., Ed. Espec., Lavras, Brazil, 2002, 1403–1409.
Yancheva S.D., Golubowicz Z., Yablowicz Z., Perl A., Flaischman M.A., Efficient Agrobacterium-mediated transformation and recovery of transgenic fig (Ficus carica L.) Plant Sci. 168 (2005) 1433–1441.
Korkmaz S., Double stranded RNA (dsRNA), Analizi Yöntemiyle Bitki Virüslerinin Teshisi ÇÜZF, Derg. 14 (3) (1999) 1–6 (in Turkish).