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Influence of curing procedures on sensory quality of vanilla beans

Published online by Cambridge University Press:  06 December 2010

Sahar Van Dyk
Affiliation:
Univ. Western Sydney, Cent. Plants Environ., Locked Bag 1797, Penrith South DC, NSW 1797, Australia
William Barry McGlasson
Affiliation:
Univ. Western Sydney, Cent. Plants Environ., Locked Bag 1797, Penrith South DC, NSW 1797, Australia
Mark Williams
Affiliation:
Univ. Western Sydney, School Nat. Sci., Locked Bag 1797, Penrith South DC, NSW 1797, Australia
Cathy Gair
Affiliation:
Former., Sensory Solutions Ltd., Northmead, NSW 2145, Australia; current address, 12 Partridge Ave., Castle Hill, NSW 2154, Australia
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Abstract

Introduction. During maturation, vanillin is accumulated in green vanilla beans as glucovanillin; it is hydrolyzed to free vanillin by endogenous glucosidases during curing and gives the characteristic flavor of vanilla. The objective of our study was to investigate methods of curing that could greatly reduce the time to complete the process and yield cured beans that retain high concentrations of vanillin and other flavor compounds with high sensory quality rating. Materials and methods. Mature green beans were obtained from a commercial grower (Cairns, Queensland, Australia). One batch of beans was continuously sweated at 35 °C at high relative humidity (RH) for 12 d. Two further batches were blanched in water at 67 °C for 3 min, and then sweated at 45 °C at high RH for 4 d or at 35 °C for 5 d. The beans were sweated until they turned brown. Three methods of drying were evaluated: a heat pump dryer at 40 °C and RH 15%, tunnel dryer at 60 °C and RH 20%, and tunnel dryer at 60 °C and RH 10%. Vanillin was extracted from powdered samples of beans with n-pentane and dichloromethane (1:1 v/v) and assayed by HPLC. Glucovanillin was measured as total vanillin after acid hydrolysis of powdered samples of beans. Results and discussion. About 90% of the glucovanillin was converted to vanillin in non-blanched beans continuously sweated at 35 °C, but there was only 70% conversion in beans blanched at 67 °C for 3 min and sweated at 45 °C for 4 d or at 35 °C for 5 d. The sensory quality of cured beans was assessed by untrained panelists. Profiling showed that the beans sweated continuously at 35 °C had superior aroma compared with beans blanched in hot water and sweated at 45 °C or at 35 °C but the appearance of non-blanched beans was less attractive. Conclusion. The study revealed that a mild hot water blanching treatment followed by sweating at 35–45 °C and rapid drying is required to produce cured beans with excellent appearance and attractive aroma.

Type
Original article
Copyright
© 2010 Cirad/EDP Sciences

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References

Childers N.F., Cibes H.R., Hernandez-Medina E., Vanilla – the orchid of commerce, in: Withner C.L., Robert E. (Eds.), The Orchids, A scientific survey, Krieger Publ. Co., Malabar, Fla., U.S.A., 1959, 477–508.
Kanisawa, T., Flavor development in vanilla beans, Kouryou 180 (1993) 113123. Google Scholar
Dignum, M.J.W., Van Der Heijden, R., Kerler, J., Winkel, C., Verpoorte, R., Identification of glucosides in green beans of Vanilla planifolia Andrews and kinetics of vanilla β-glucosidase, Food Chem. 85 (2004) 199205. CrossRefGoogle Scholar
Havkin-Frenkel D., Podstolski A., Witkowska E., Molecki, P., Mikolajczyk M., Vanillin biosynthetic pathways, an overview, in: Fu T.J., Singh G., Curtis W.R., (Eds.), Plant cell and tissue culture for the production of food ingredients, Kluwer Acad. Press / Plenum Publ., N.Y., U.S.A., 1999, 35–43.
Kanisawa T., Tokoro k., Kawahara S., Flavor development in the beans of Vanilla planifolia Andrews, in: Kurihara K., Suzuki N., Ogawa H. (Eds.), Olfaction taste XI, Proc. Int. Symp. Tokyo, Springer, Japan, 1994, pp. 268–270.
Ranadive A.S., Szkutnica K., Guerrera J.G., Frenkel C., Vanillin biosynthesis in vanilla beans, in: Proc. IX Int. Congr. Essent. Oils, Singap., 1983, pp. 147–154.
Havkin-Frenkel, D., French, J.C., Pak, F.E., Frenkel, C., Inside vanilla: Vanilla planifolia’s botany, curing options and future market prospects, Perfum. Flavour. 30 (2005) 3655. Google Scholar
Joel, D.M., French, J.C., Graft, N., Korteva, G., Dixon, R.A., Havkin-Frenkel, D., A hairy tissue produces vanillin, Isr. J. Plant Sci. 51 (2003) 157159. CrossRefGoogle Scholar
Odoux, E., Escoute, J., Verdeil, J.L., Brillouet, J.M., Localization of β-D-glucosidase activity and glucovanillin in vanilla bean, Ann. Bot. 92 (2003) 437444. CrossRefGoogle ScholarPubMed
Odoux, E., Brillouet, J.M., Anatomy, histochemistry and biochemistry of glucovanillin, oleoresin and mucilage accumulation sites in green mature vanilla pod (Vanilla planifolia; Orchidaceae): a comprehensive and critical reexamination, Fruits 64 (2009) 221241. CrossRefGoogle Scholar
Havkin-Frenkel, D., French, J.C., Graft, N.M., Pak, F.E., Frenkel, C., Joel, D.M., Interrelation of curing and botany in vanilla (Vanilla planifolia) bean, Acta Hortic. 629 (2004) 93102. CrossRefGoogle Scholar
Mariezcurrena, M.D., Zavaleta, H., Waliszewski, K.N., Sanchez, V., The effect of killing conditions on the structural changes in vanilla (Vanilla planifolia, Andrew) pods during the curing process, Int. J. Food Sci. Technol. 43 (2008) 14521457. CrossRefGoogle Scholar
Purseglove J.W., Brown E.G., Green C.L., Robins S.R.J., Spices, in: Tropical agricultural, Longman Inc., N.Y., U.S.A., 1981, pp. 644–735.
Havkin-Frenkel, D., Frenkel, C., Postharvest handling and storage of cured vanilla beans, Stewart Postharvest Rev. 4 (2006) 19. Google Scholar
Jones, M.A., Vicente, G.C., Criteria for testing vanilla in relation to killing and curing methods, Agric. Res. 78 (1948) 425434. Google Scholar
Theodose, R., Traditional methods of vanilla preparation and their improvement, Tropic. Sci. 15 (1973) 4757. Google Scholar
Dignum, M.J.W., Kerler, J., Verpoorte, R., Vanilla curing under laboratory conditions, Food Chem. 79 (2002) 165171. CrossRefGoogle Scholar
Odoux, E., Changes in vanillin and glucovanillin concentrations during the various stages of the process traditionally used for curing of Vanilla fragrans beans in Réunion, Fruits 55 (2000) 119125. Google Scholar
Dignum, M.J.W., Kerler, J., Verpoorte, R., Vanilla production: technological chemical and biosynthetic aspects, Food Rev. Int. 17 (2001) 199219. CrossRefGoogle Scholar
Wild-Altamirano, C., Enzymatic activity during growth of vanilla fruit. 1. Proteinase, glucosidase, peroxidase and polyphenoloxidase, J. Food Sci. 34 (1969) 235238. CrossRefGoogle Scholar
Hanum, T., Changes in vanillin and activity of β-glucosidase and oxidases during postharvest processing of vanilla beans (Vanilla planifolia), Bull. Teknol. Ind. Pangan 8 (1997) 4652. Google Scholar
Marquez, O., Waliszewski, K.N., The effect of thermal treatment on β-glucosidase inactivation in vanilla bean (Vanilla planifolia Andrew ), Int. J. Food Sci. Technol. 43 (2008) 19931999. CrossRefGoogle Scholar
Odoux, E., Chauwin, A., Brillouet, J.-M., Purification and characterization of vanilla bean (Vanilla planifolia Andrews) ß–D-glucosidase, J. Agric. Food Chem. 51 (2003) 31683173. CrossRefGoogle Scholar
Odoux, E., Escoute, J., Verdeil, J.L., The relation between glucovanillin, ß-D-glucosidase activity and cellular compartmentation during the senescence, freezing and traditional curing of vanilla beans, Ann. Appl. Biol. 149 (2006) 4352. CrossRefGoogle Scholar
Cervantes L., Azuara E., Beristain, C.I., An improvement in the curing process of vanilla beans, Food Chem. Conf.: Chemical effects of food processing and preservation, Las Vegas, U.S.A., 2004.
Perez-Silva, A., Osouz, E., Brat, P., Riveyre, F., Rodriguez-Jimenes, G., Robles-Olvera, V., Garcia-Alvarado, M.A., Gunata, Z., GC-MS and GC-olfactometry analysis of aroma compounds in a representative organic aroma extract from cured vanilla (Vanilla planifolia G. Jackson) beans, Food Chem. 99 (2006) 728735. CrossRefGoogle Scholar
Adedeji, J., Hartman, T.G., Ho, C.-T., Flavour characterization of different varieties of vanilla beans, Perfum. Flavour. 18 (1993) 2533. Google Scholar
Arana F.E., Vanilla curing and its chemistry, USDA Fed. Exp. Stn., Mayaguez, USDA, P.R. Bull. No. 42, Wash. D.C., U.S.A., 1944.
Rivera J.G., Hageman R.H., Vanilla curing, USDA Rep. Fed. Exp. Stn., Mayaguez, USDA, P.R., 1950.
Sreedhar, R.V., Roohie, K., Venkatachalam, L., Narayan, M.S., Bhagyalakshmi, N., Specific pre-treatments reduce curing period of Vanilla (Vanilla planifolia) beans, J. Agric. Food Chem. 55 (2007) 29472955. CrossRefGoogle Scholar
Gatfield, I., Reib, I., Krammer, G., Schmidt, C.O., Kindel, G., Bertram, H.J., Divanillin: novel taste-active component of fermented vanilla beans, Perfum. Flavour. 1 (2006) 1820. Google Scholar
Dignum, M.J.W., Kerler, J., Verpoorte, R., ß-glucosidase and peroxidase stability in crude enzyme-extracts from green beans of Vanilla planifolia Andrews, Phytochem. Anal. 12 (2001) 174179. CrossRefGoogle Scholar