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Effect of the combined inoculation of arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria on papaya (Carica papaya L.) infected with the root-knot nematode Meloidogyne incognita

Published online by Cambridge University Press:  30 June 2006

María del Carmen Jaizme-Vega ,
Ana Sue Rodríguez-Romero  and
Luis Antonio Barroso Núñez
María del Carmen Jaizme-Vega
Affiliation:
Dpto. Protección Vegetal, Instituto Canario Investigaciones Agrarias, Apdo. 60, 38200 La Laguna, Tenerife, España
Ana Sue Rodríguez-Romero
Affiliation:
Dpto. Protección Vegetal, Instituto Canario Investigaciones Agrarias, Apdo. 60, 38200 La Laguna, Tenerife, España
Luis Antonio Barroso Núñez
Affiliation:
Dpto. Protección Vegetal, Instituto Canario Investigaciones Agrarias, Apdo. 60, 38200 La Laguna, Tenerife, España
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Abstract

Introduction. Arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) can be considered important rhizospheric beneficial microorganisms. Their use as biocontrol strategies against soilborne pathogens such as nematodes should be taken into account. However, optimal management of soil microbiota communities is not easy because of the high specificity involved in these types of interactions. The aim of our study was to determine whether the combined inoculation of two AMF species and a Bacillus consortium based on three strains previously described as PGPR in other crops were able to reduce nematode infection and damage on papaya. Materials and methods. Papaya seedlings were inoculated with two AMF isolates (Glomus mosseae or G. manihotis) at the beginning of the nursery phase. Once the mycorrhizal symbiosis was established, a Bacillus consortium was applied. Nematode inoculum was applied 20 d after transplanting to individual pots. Plants were harvested 160 d after nematode inoculation. Results. In terms of plant development and nutrition, benefits due to AMF inoculation persisted in the presence of PGPR. However, the effect of dual inoculation was different, depending on the Glomus species. This positive effect was also evident in plants with nematode. Meloidogyne infection was significantly reduced in mycorrhizal plants. However, the addition of PGPR does not seem to improve the results of AMF single treatments in terms of nematode infection. Conclusion. Dual application of AMF and PGPR must be considered for papaya threatened by the root-knot nematode, although a previous screening should be done in order to select the best microbe combination to optimise results.

Keywords

SpainCarica papayaarbuscular mycorrhizaeGlomusrhizobacteriaBacillussymbiosisgrowthplant nutritionbiological controlEspagneCarica papayamycorhize à arbusculeGlomusrhizobactérieBacillussymbiosecroissancenutrition des planteslutte biologique
Type
Research Article
Information
Fruits , Volume 61 , Issue 3 , May 2006 , pp. 151 - 162
Copyright
© CIRAD, EDP Sciences, 2006

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