Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-25T22:06:23.606Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of oligochitosan on development of Colletotrichum musae in vitro and in situ and its role in protection of banana fruits

Published online by Cambridge University Press:  06 April 2012

Meng Xiangchun ,
Tang Yanxia ,
Zhang Aiyu ,
Huang Xuemei  and
Zhang Zhaoqi
Meng Xiangchun
Affiliation:
Inst. Fruit Tree Res., Guangdong Acad. Agric. Sci., Guangzhou, P.R. China
Tang Yanxia
Affiliation:
Inst. Fruit Tree Res., Guangdong Acad. Agric. Sci., Guangzhou, P.R. China
Zhang Aiyu
Affiliation:
Inst. Fruit Tree Res., Guangdong Acad. Agric. Sci., Guangzhou, P.R. China
Huang Xuemei
Affiliation:
Coll. Hortic., S. China Agric. Univ., Guangzhou, P.R. China. [email protected]
Zhang Zhaoqi*
Affiliation:
Coll. Hortic., S. China Agric. Univ., Guangzhou, P.R. China. [email protected]
*
Correspondence and reprints
Get access

Abstract

Introduction. Concerns about the potentially harmful effects of fungicides on human health and the environment encourage the search for alternative treatments for perishable fruit postharvest disease control. To this end, the potential use of oligochitosan as a natural antifungal compound to control postharvest anthracnose caused by Colletotrichum musae was investigated in banana fruits from the Cavendish group (genome AAA). Materials and methods. The influence of oligochitosan on the growth of C. musae was determined in vitro by micrographic analysis, while its in situ antifungal activity was monitored in banana fruits that were artificially injury-inoculated with C. musae; the activities of several defense-related enzymes were measured. Results and discussion. Oligochitosan at (4 and 8) g·L–1 markedly inhibited radial mycelial growth of C. musae in vitro. The scanning electron micrograph of C. musae treated with oligochitosan at inhibitory concentrations showed distortion and thinning of the hyphal wall and reduction in fungus colony diameter. Dipping banana fruits in oligochitosan solution at (5 to 20) g·L–1 significantly reduced the diameter of the anthracnose lesion, and 20 g oligochitosan·L–1 almost reached the same inhibitory effect as 0.5 g·L–1 of Sportak®, a synthetic fungicide. Activities of defense-related enzymes such as phenylalanine ammonia-lyase (PAL), β-1, 3-glucanase (GLU) and chitinase (CHT), but not polyphenol oxidase (PPO), increased in banana fruits treated with 0.5 g oligochitosan·L–1. Conclusion. The inhibitory effect of oligochitosan on anthracnose development is due to the combination of a direct antifungal effect on the pathogen and an indirect effect, whereby the activities of defense-related enzymes in the banana fruit were enhanced. To control anthracnose in harvested bananas, treatment with oligochitosan above 20 g·L–1 may substitute the use of synthetic fungicide.

Keywords

ChinaMusa (bananas)postharvest lossesdisease controlbiological controlanthracnosisoligochitosanantifungal propertiesinduced resistanceChineMusa (bananes)perte après récoltecontrôle de maladieslutte biologiqueanthracnoseoligochitosanepropriété antifongiquerésistance induiteChinaMusa (bananos)pérdidas postcosechacontrol de enfermedadescontrol biológicoantracnosisoligoquitosanopropiedades antimicosicasresistencia inducida
Type
Original article
Information
Fruits , Volume 67 , Issue 3 , May 2012 , pp. 147 - 155
Copyright
© 2012 Cirad/EDP Sciences

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

De Costa, D.M.,Erabadupitiya, H.R.U.T., An integrated method to control postharvest diseases of banana using a member of the Burkholderia cepacia complex, Postharvest Biol. Technol. 36 (2005) 31–39.CrossRefGoogle Scholar
Finlay, A.R.,Brown, A.E., The relative importance of Colletotrichum musae as a crown rot pathogen on Windward Island bananas, Plant Pathol. 42 (1993) 67–74.CrossRefGoogle Scholar
Khan, S.H.,Aked, J.,Magan, N., Control of the anthracnose pathogen of banana (Colletotrichum musae) using antioxidants alone and in combination with thiabendazole or imazalil, Plant. Pathol. 50 (2001) 601–608.CrossRefGoogle Scholar
Maqbool, M.,Ali, A.,Alderson, P.G., Effect of cinnamon oil on incidence of anthracnose disease and postharvest quality of bananas during storage, Int. J. Agric. Biol. 12 (2010) 516–520.Google Scholar
Eryani, A.A.,Mahmud, T.M.M.,Syed, S.R.,Mohamed, A.R.,Eryani, A.R., Effects of calcium and chitosan treatments on controlling anthracnose and postharvest quality of papaya (Carica papaya L.), Int. J. Agric. Res. 4 (2009) 53–68.CrossRefGoogle Scholar
Jitareerat, P.,Paumchai, S.,Kanlayanarat, S.,Sangchote, S., Effect of chitosan on ripening, enzymatic activity, and disease development in mango (Mangifera indica) fruit, N. Z. J. Crop Hortic. Sci. 35 (2007) 211–218.CrossRefGoogle Scholar
Win, N.K.K.,Jitareerat, P.,Kanlayanarat, S.,Sangchote, S., Effects of cinnamon extract, chitosan coating, hot water treatment and their combinations on crown rot disease and quality of banana fruit, Postharvest Biol. Technol. 45 (2007) 333–340.CrossRefGoogle Scholar
Liu, J.,Tian, S.P.,Meng, X.H.,Xu, Y., Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit, Postharvest Biol. Technol. 4 (2007) 300–306.CrossRefGoogle Scholar
Han, C.,Zhao, Y.,Leonard, S.W.,Traber, M.G., Edible coatings to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria × ananassa) and raspberries ( Rubus idaeus), Postharvest Bio. Technol. 33 (2004) 67–78.CrossRefGoogle Scholar
Bautista-Baños, S.,Hernandez, A.N.,Valle, M.G.,Hernandez, M.,Barka, E.A.,Bosquez, E.,Wilson, C.L., Chitosan as a potential natural compound to control pre and post harvest diseases of horticultural commodities, Crop Prot. 25 (2006) 108–118.CrossRefGoogle Scholar
Meng, X.,Li, B.,Liu, J.,Tian, S., Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage, Food Chem. 106 (2008) 501–508. CrossRefGoogle Scholar
Zhao, X.M.,She, X.P.,Du, Y.G.,Liang, X.M., Induction of antiviral resistance and stimulary effect by oligochitosan in tobacco, Pestic. Biochem. Physiol. 87 (2007) 78–84.CrossRefGoogle Scholar
Liu, H.M.,Cheng, S.H.,Liu, J.,Du, Y.G.,Bai, Z.H.,Du, Y.G., Synthesis of pentasaccharide and heptasaccharide derivatives and their effects on plant growth, J. Agric. Food Chem. 56 (2008) 5634–5638. CrossRefGoogle ScholarPubMed
Zhang, M.,Tan, T.,Yuan, H.,Rui, C., Insecticidal and fungicidal activities of chitosan and oligo-chitosan, J. Bioact. Compat. Polym. 18 (2003) 391–400.CrossRefGoogle Scholar
Meng, X.,Yang, L.,Kennedy, J.F.,Tian, S., Effects of chitosan and oligochitosan on growth of two fungal pathogens and physiological properties in pear fruit, Carbohydr. Polym. 81 (2010) 70–75.CrossRefGoogle Scholar
Kim, S.K.,Rajapakse, N., Enzymatic production and biological activities of chitosan oligosaccharides (COS): A review, Carbohydr. Polym. 62 (2005) 357–368.CrossRefGoogle Scholar
Yao, H.J.,Tian, S.P., Effects of pre- and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage, Postharvest Biol. Technol. 35 (2005) 253–262.CrossRefGoogle Scholar
Alvindia, D.G.,Natsuaki, K.T., Biocontrol activities of Bacillus amyloliquefaciens DGA14 isolated from banana fruit surface against banana crown rot-causing pathogens, Crop Prot. 28 (2009) 236–242.CrossRefGoogle Scholar
Jullien, A.,Chillet, M.,Malezieux, E., Pre-harvest growth and development, measured as accumulated degree days, determine the post-harvest green life of banana fruit, J. Hortic. Sci. Biotechnol. 83 (2008) 506–512.CrossRefGoogle Scholar
Lisker, N.,Cohen, L.,Chalutz, E.,Fuchs, Y., Fungal infections suppress ethylene-induced phenylalanine ammonia lyase activity in grapefruits, Physiol. Mol. Plant Pathol. 22 (1983) 331–338.CrossRefGoogle Scholar
Deepak, S.,Niranjan, S.,Shailasree, S.,Kini, R.K.,Boland, W.,Shetty, H.S.,Mithofer, A., Induction of resistance against downy mildew pathogen in pearl millet by a synthetic jasmonate analogon, Physiol. Mol. Plant Pathol. 71 (2007) 96–105.CrossRefGoogle Scholar
Zhao, X.M.,She, X.P.,Yu, W.,Liang, X.M.,Du, Y.G., Effects of oligochitosans on tobacco cells and role of endogenous nitric oxide burst in the resistance of tobacco to tobacco mosaic virus, J. Plant Pathol. 89 (2007) 55–65.Google Scholar
Kasprzewska, A., Plant chitinases-regulation and function, Cell. Mol. Biol. Lett. 8 (2003) 809–824.Google ScholarPubMed
Wang, J.,Wang, B.,Jiang, W.,Zhao, Y., Quality and shelf life of mango (Mangifera indica L. cv. Tainong) coated by chitosan and polyphenols, Food Sci. Technol. Int. 13 (2007) 317–322.CrossRefGoogle Scholar