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Determination of citrus fruit origin by using 16S rDNA fingerprinting of bacterial communities by PCR- DGGE: an application to clementine from Morocco and Spain

Published online by Cambridge University Press:  13 March 2008

Doan Duy Le Nguyen ,
Elodie Gemrot ,
Gérard Loiseau  and
Didier Montet
Doan Duy Le Nguyen
Affiliation:
CIRAD, PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France Cantho University, College of Agriculture and Applied Biology, Rue 3-2, Campus 2, Ninh Kieu district, Cantho province, Viet Nam
Elodie Gemrot
Affiliation:
CIRAD, PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France
Gérard Loiseau
Affiliation:
CIRAD, PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France
Didier Montet
Affiliation:
CIRAD, PERSYST, UMR Qualisud, TA B-95/16, 34398 Montpellier Cedex 5, France
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Abstract

Introduction. Consumption of citrus fruits and their juice has strongly grown in the last few decades. However, at the present time, the traceability of these fruits is only documentary. In case of doubt or fraud, no standardized analysis makes it possible to discriminate or determine the geographical origin of culture of the fruit. Materials and methods. A method of bacterial ecology, PCR-DGGE, was used to characterize the bacterial flora of clementines imported into France from Spain and Morocco in order to show that there is a relation between the bacterial communities of the fruits and their geographical origins. The principle rests on the determination of specific biological markers for a given region. Protocols of microbial extraction and DNA amplification were optimized. Results. DGGE profiles analyzed by multivariate analysis permitted us to distinguish microbial communities from different origins. Conclusion. We propose the PCR-DGGE method as a new tool for traceability that provides citrus products with a unique bar code and makes it possible to trace back the citrus fruit to their original location.

Keywords

MoroccoSpainCitrus reticulatafruitsprovenancemicrobial proteinsDNAPCRDNA fingerprintingMarocEspagneCitrus reticulatafruitsprovenanceprotéine microbienneADNPCRempreinte ADN
Type
Research Article
Information
Fruits , Volume 63 , Issue 2 , March 2008 , pp. 75 - 84
Copyright
© CIRAD, EDP Sciences, 2008

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