Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T18:54:39.207Z Has data issue: false hasContentIssue false
  • English
  • Français

Transferability of SSR markers from lychee (Litchi chinensis Sonn.) to pulasan (Nephelium ramboutan-ake L.)

Published online by Cambridge University Press:  08 February 2006

Chun Hock Sim ,
Mansor Clyde Mahani ,
Chee Yen Choong  and
Idris Salma
Chun Hock Sim
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Mansor Clyde Mahani
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Chee Yen Choong
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Idris Salma
Affiliation:
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Get access

Abstract

Introduction. Pulasan and lychee are from the same family and closely related, therefore the SSR markers are expected to be highly transferable between these two taxa. We investigated the transferability of 12 lychee (Litchi chinensis Sonn.) simple sequence repeat (SSR) loci to pulasan (Nephelium ramboutan-ake L.). Materials and methods. Genomic DNA was extracted from 20 accessions of pulasan for the PCR amplification of the SSR loci using 12 pairs of SSR primers derived from lychee. The PCR products were resolved on denaturing polyacrylamide gels. Results. The percentage of SSR transferability from lychee to pulasan was 58.3% and the percentage of polymorphic SSR markers was 25%. Discussion. The moderate transferability and low polymorphism rates suggest the possibility of interruptions within the repeat motif and mutations in the flanking sequences of SSR repeat motifs. Our results did not reveal a high rate of transferability between lychee and pulasan. However, this study showed that the SSR markers developed in lychee are a good source of molecular markers for pulasan.

Keywords

MalaysiaLitchi chinensisNephelium ramboutan-akemolecular biologygenetic markersPCRgenetic polymorphismMalaisieLitchi chinensisNephelium ramboutan-akebiologie moléculairemarqueur génétiquePCRpolymorphisme génétique
Type
Research Article
Information
Fruits , Volume 60 , Issue 6 , November 2005 , pp. 379 - 385
Copyright
© CIRAD, EDP Sciences, 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adema F., Leenhouts P.W., van Welzen P.C., Flora Malesiana, Series I, Spermatophyta: flowering plants, Vol. 11, part 3: Sapindaceae, Rijksherbarium, Leiden, Netherlands, 1994, pp. 419–768.
Aman R., Buah-buahan nadir Semenanjung Malaysia, Dewan Bahasa dan Pustaka, Kuala Lumpur, 1992.
Kuleung, C., Baenziger, P.S., Dweikat, I., Transferability of SSR markers among wheat, rye and triticale, Theor. Appl. Genet. 108 (2004) 1147–1150. CrossRef
Fitzsimmons, N.N., Moritz, C., Moore, S.S., Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution, Mol. Biol. Evol. 12 (1995) 432–440.
Szewc-McFadden, A.K., Kresovich, S., Bliek, S.M., Mitchell, S.E., McFerson, J.R., Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species, Theor. Appl. Genet. 93 (1996) 534–538. CrossRef
Smulders, M.J.M., Bredemeijer, G., Ruskortekaas, W., Arens, P., Vosman, B., Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species, Theor. Appl. Genet. 94 (1997) 264–272. CrossRef
Rallo, P., Tenzer, I., Gessler, C., Baldoni, L., Transferability of olive microsatellite loci across the genus Olea, Theor. Appl. Genet. 107 (2003) 940–946. CrossRef
Eujayl, I., Sledge, M.K., Wang, L., May, G.D., Chekhovskiy, K., Zwonitzer, J.C., Mian, M.A.R., Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp., Theor. Appl. Genet. 108 (2004) 414–422. CrossRef
Brondani, C., Rangel, P.H.N., Borba, T.C.O., Brondani, R.P.V., Transferability of microsatellite and sequence tagged site markers in Oryza species, Hereditas 138 (2003) 187–192. CrossRef
Cordeiro, G.M., Pan, Y.B., Henry, R.J., Sugarcane microsatellites for the assessment of genetic diversity in sugarcane germplasm, Plant Science 165 (2003) 181–189. CrossRef
Yazdani, R., Scotti, I., Jansson, G., Plomion, C., Mathur, G., Inheritance and diversity of simple sequence repeat (SSR) microsatellite markers in various families of Picea abies, Hereditas 138 (2003) 219–227. CrossRef
Viruel, M.A., Hormaza, J.I., Development, characterization and variability analysis of microsatellites in lychee (Litchi chinensis Sonn., Sapindaceae), Theor. Appl. Genet. 108 (2004) 896–902. CrossRef
Doyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue, Focus 12 (1990) 13–15.
Sambrook J., Russel D.W., Molecular cloning: a laboratory manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001.
González-Martínez, S.C., Robledo-Arnuncio, J.J., Collada, C., Díaz, A., Willioms, C.G., Alía, R., Cervera, M.T., Cross-amplification and sequence variation of microsatellite loci in Eurasian hard pines, Theor. Appl. Genet. 109 (2004) 103–111.
Joseph S.S., The analysis of microsatellites by silver staining of nondenaturing polyacrylamide gels, in: Tietz D., Nucleic acid electrophoresis, Springer-Verlag, Berlin, Germany, 1998.
Barreneche, T., Casasoli, M., Russell, K., Akkak, A., Meddour, H., Plomion, C., Villani, F., Kremer, A., Comparative mapping between Quercus and Castanea using simple-sequence repeats (SSRs), Theor. Appl. Genet. 108 (2004) 558–566. CrossRef
Saha, M.C., Mian, M.A.R., Eujayl, I., Zwonitzer, J.C., Wang, L., May, G.D., Tall fescue EST-SSR markers with transferability across several grass species, Theor. Appl. Genet. 109 (2004) 783–791. CrossRef
Taylor, J.S., Durkin, J.M.H., Breden, F., The death of a microsatellite: a phylogenetic perspective on microsatellite interruptions, Mol. Biol. Evol. 16 (1999) 567–571. CrossRef
Peakall, R., Gilmore, S., Keys, W., Morgante, M., Rafalski, A., Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants, Mol. Biol. Evol. 15 (1998) 1275–1287. CrossRef
Pépin, L., Amigues, Y., Lepingle, A., Berthier, J.L., Bensaid, A., Vaiman, D., Sequence conservation of microsatellites between Bos Taurus (cattle), Capra hircus (goat) and related species: examples of use in parentage testing and phylogeny analysis, Heredity 74 (1995) 52–61. CrossRef
Scott, K.D., Eggler, P., Seaton, G., Rossetto, M., Ablett, E.M., Lee, L.S., Henry, R.J., Analysis of SSRs derived from grape ESTs, Theor. Appl. Genet. 100 (2000) 723–726. CrossRef