Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-05T11:27:52.812Z Has data issue: false hasContentIssue false

Spread of cymbidium mosaic potexvirus and potyvirusesin vanilla plants grown in shade houses in Reunion Island

Published online by Cambridge University Press:  15 April 2002

Françoise Leclercq-Le Quillec
Affiliation:
Cirad-Flhor, BP 180, 97455 Saint-Pierre, Ile de la Réunion
Claude Rivière
Affiliation:
Cirad-Flhor, BP 180, 97455 Saint-Pierre, Ile de la Réunion
Anne Lagorce
Affiliation:
Cirad-Flhor, BP 180, 97455 Saint-Pierre, Ile de la Réunion
Get access

Abstract

Introduction. Beginning in 1994, vanilla vines grown under shade houses in Reunion Island have developed disorders mainly due to the cymbidium mosaic potexvirus (CymV) and potyviruses. To reduce damage and avoid viral epidemics, we considered developing a vanilla nursery to supply farmers with virus-free plant material and to provide prophylactic advice. Accordingly, we determined the relationship between symptoms on vanilla leaves and viral diagnosis. We also determined the temporal and spatial distribution patterns of viral diseases. Materials and methods. Symptom assessment and serological tests were carried out on vanilla leaves (Vanilla fragrans) at various times. The relationship between symptoms and viral infection data was analyzed with Statistica software. Spatial distribution patterns of infected plants were generated with two-dimensional distance class analysis (2DClass). Results and discussion. Results showed that symptoms do not correlate well with serological diagnosis. Plants in all shade houses in the survey were infected mainly by CymV which spreads in the row according to cultural practices. Potyviruses could be transmitted both by insect vectors and mechanically, but for the latter case less efficiently than CymV. Conclusion. Results from this study can be used to develop control measures, including the production of healthy planting materials to manage plant disease epidemics. Further studies will involve the characterization of vanilla potyviruses so that reliable serological techniques and better molecular tools can be developed.

Type
Research Article
Copyright
© CIRAD, EDP Sciences

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Côme B., Gambin O., Synthèse des premiers travaux sur les viroses des cultures de vanille sous ombrière, Provanille-FDGDEC, St-Pierre, île de la Réunion, 1997, 10 p.
Pearson M.N., Assessment of vanilla virus problem in île de la Réunion, Rapport de mission, Auckland Univ., New Zealand, 1997, 17 p.
Wisler, G.C., Zettler, F.W., Purcifull, D.E., Two viruses of Vanilla in Society Islands, Abstr., Phytopathology 76 (1986) 1091.
Pearson, M.N., Pone, S.P., Viruses of vanilla in the Kingdom of Tonga, Aust. Plant Pathol. 17 (3) (1988) 59-60. CrossRef
Wisler, G.C., Zettler, F.W., Mu, L., Virus infections of vanilla and other orchids in French Polynesia, Plant Dis. 71 (1987) 1125-1129. CrossRef
Zettler, F.W., Ko, N.J., Wisler, G.C., Elliot, M., Wong, S.M., Viruses of orchids and their control, Plant Dis. 74 (1990) 621-625. CrossRef
Pearson, M.N., Brunt, A.A., Pone, S.P., Some hosts and properties of a potyvirus infecting Vanilla fragrans (Orchidaceae) in the Kingdom of Tonga, J. Phytopathol. 128 (1990) 46-54. CrossRef
Pearson, M.N., Jackson, G.V.H., Pone, S.P., Howitt, R.L.J., Vanilla viruses in the South Pacific, Plant Pathol. 42 (1) (1993) 127-131. CrossRef
Wang, Y.Y., Pearson, M.N., Some characteristics of potyvirus isolates from Vanilla tahitensis in French Polynesia and the Cook Islands, J. Phytopathol. 135 (1992) 71-76. CrossRef
Bénézet, H., Picard, E., Côme, C., Grisoni, G., Leclercq-Le Quillec, F., Gambin, O., Jeuffrault, E., Les virus du vanillier à la Réunion : un frein au développement de la culture sous ombrière, Phytoma 526 (2000) 40-42.
Jordan, R., Hammond, J., Comparison and differentiation of potyvirus group-common epitopes using monoclonal antibodies, J. Gen. Virol. 72 (1991) 25-36. CrossRef
Maury, Y., Bossennec, J.M., Boudazin, G., Hampton, R.O., Pietersen, G., Maguire, J.D., Factors influencing Elisa evaluation of transmission of pea seed-borne mosaic virus in infected pea seed: seed group size and seed decortication, Agronomie 7 (4) (1987) 225-230. CrossRef
Ferrandino, F.J., Two dimensional distance class analysis of disease-Incidence data: problems and possible solutions, Phytopa-thology 86 (7) (1996) 685-691.
Liefting, L., Pearson, M.N., Pone, S.P., The isolation and evaluation of two naturally occurring mild strains of vanilla necrosis potyvirus for control by cross-protection, J. Phytopa-thol. 136 (1992) 9-15. CrossRef