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Methylation-sensitive amplification polymorphism (MSAP) protocol to assess CpG and CpNpG methylation in the banana genome

Published online by Cambridge University Press:  13 March 2008

Franc-Christophe Baurens ,
Sandrine Causse  and
Thierry Legavre
Franc-Christophe Baurens
Affiliation:
CIRAD-Bios, UMR DAP, TA A-96 / 03, Avenue Agropolis, 34398 Montpellier Cedex 5, France [email protected], [email protected]
Sandrine Causse
Affiliation:
CIRAD-Bios, UMR DAP, TA A-96 / 03, Avenue Agropolis, 34398 Montpellier Cedex 5, France [email protected], [email protected]
Thierry Legavre
Affiliation:
CIRAD-Bios, UMR DAP, TA A-96 / 03, Avenue Agropolis, 34398 Montpellier Cedex 5, France [email protected], [email protected]
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Abstract

Introduction. The technique presented provides molecular markers for DNA methylation studies in the Musa sp. genome. This technique can be used for studying DNA methylation in natural populations, in mapping populations, and also in plant organs or during plant development. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. This part describes the required materials, and the protocols for producing MSAP autoradiography with HpaII / MspI (CCGG methylation) and for EcoRII / PspGI (CCWGG methylation). It mentions the main problems which can occur. Results. The protocol makes it possible to detect CCWGG and CCGG methylated sites.

Keywords

FranceMusa sp.methodsmethylationDNAmarqueur génétiqueFranceMusa sp.méthodeméthylationADNgenetic markers
Type
Research Article
Information
Fruits , Volume 63 , Issue 2 , March 2008 , pp. 117 - 123
Copyright
© CIRAD, EDP Sciences, 2008

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References

Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., AFLP: a new technique for DNA fingerprinting, Nucleic Acids Res. 23 (21) (1995) 4407–4414. CrossRef
Reyna-López, G.E., Simpson, J., Ruiz-Herrera, J., Differences in DNA methylation pattern are detectable during the dimorphic transition of fungi by amplification of restriction polymorphisms, Mol. Gen. Genet. 253 (1997) 703–710.
Xiong, L.Z., Xu, C.G., Saghai-Maroof, M.A., Zhang, Q., Patterns of cytosine methylation in an elite rice hybrid and its parental lines, detected by a methylation-sensitive amplification polymorphism technique, Mol. Gen. Genet. 261 (1999) 439–446. CrossRef
Schmitt, F., Oakeley, E.J., Jost, J.P., Antibiotics induce genome-wide hypermethylation in cultures of Nicotiana tabacum plants, J. Biol. Chem. 272 (3) (1997) 1534–1540. CrossRef
Zhou, Y., Magill, C.W., Magill, J.M., Newton, R.J., An apparent case of nonsymetrical and sustained strand-specific hemimethylation in the Dc8 gene of carrot, Genome 41 (1998) 23–33. CrossRefPubMed