Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-23T03:39:32.082Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of cytokinins on morphological, physiologicaland biochemical characteristics of shoots of citrus in vitro

Published online by Cambridge University Press:  15 June 2002

Villupanoor Alwar Parthasarathy ,
Anjan Barua ,
Venketaswamy Nagaraju  and
U. Utpala Parthasarathy
Villupanoor Alwar Parthasarathy
Affiliation:
Central Plantation Crops Research Institute, Kasaragod 671 124, Kerala, India
Anjan Barua
Affiliation:
Biotechnology Laboratory, Division of Horticulture, ICAR Research complex for NEH Region, Barapani-793 103, Meghalaya, India
Venketaswamy Nagaraju
Affiliation:
Biotechnology Laboratory, Division of Horticulture, ICAR Research complex for NEH Region, Barapani-793 103, Meghalaya, India
U. Utpala Parthasarathy
Affiliation:
Central Plantation Crops Research Institute, Kasaragod 671 124, Kerala, India
Get access

Abstract

Introduction. Cytokinins enhance axillary shoots, which helps prolific shoot multiplication in vitro. Very few reports are available on biochemical changes associated with this in vitro proliferation of Citrus. The present study was undertaken to find out the influence of two widely used cytokinins on various morphological and biochemical constituents of in vitro proliferated shoots of Citrus species. Materials and methods. Shoots of (0.5 to 1.0) cm long from in vitro proliferated shoots of five Citrus species were cultured on MS medium supplemented with eight increasing levels of benzylaminopurine (BAP) or kinetin spread from (0.25 to 2) mg$\cdot$L-1. After 6 weeks of culture, morphogenetic characters were observed, and shoot samples were crushed with 5 mL of hot ethanol; the extract collected was used for the estimation of reducing sugars, total phenols, ortho dihydric (OD) phenol, amino nitrogen and protein percentages. Results and discussion. For all the morphogenetic characters studied, except the shoot number, there were significant interactions between the Citrus species and the different concentrations of BAP and kinetine. Increased concentration of cytokinins reduced the amount of total phenol, OD phenol and total protein. Calamondin recorded the lowest percent of reducing sugar and protein. The maximum amount of reducing sugars was recorded for C. volkameriana. Supplementation of BAP resulted in the highest amount of total and OD phenols, whereas kinetin improved the reducing sugar and amino nitrogen.

Keywords

IndiaCitrusin vitro cultureplant growth substancescytokininskinetin6-benzyladeninebiological developmentchemical compositionIndeCitrusculture in vitrosubstance de croissance végétalecytokininekinétine6-benzyladéninedéveloppement biologiquecomposition chimique
Type
Research Article
Information
Fruits , Volume 57 , Issue 3 , May 2002 , pp. 153 - 160
Copyright
© CIRAD, EDP Sciences

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Parthasarathy, V.A., Singh, I.P., Nagaraju, V., Mishra, M., Proliferating ability of shoots of certain citrus species in vitro, Ann. Plant Physiol. 12 (1996) 108-111.
Parthasarathy, V.A., Nagaraju, V., In vitro shoot proliferation in certain Citrus species, Indian J. Hortic. 53 (1996) 171-174.
Parthasarathy, V.A., Nagaraju, V., Effect of benzylaminopurine on in vitro shoot cultures of Citrus species from different mother plants, Agrotropica 10 (1998) 139-142.
Barua, A., Nagaraju, V., Parthasarathy, V.A., Micropropagation of three endangered Citrus species. 1. Shoot proliferation in vitro, Ann. Plant. Physiol. 10 (1996) 124-125.
Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plantarum 15 (1962) 473-497. CrossRef
Hazarika, B.N., Nagaraju, V., Parthasarathy, V.A., Paclobutrazol induced biochemical changes in microshoots of Citrus species, Folia Hortic. 12 (2000) 69-77.
Nelson, N., A photometric adaptation of the Somogyi methods for the determination of glucose, J. Biol. Chem. 153 (1944) 375-380.
Bray, H.G., Thorpe, W.V., Analysis of phenolic compounds on interest in metabolism, Method. Biochem. Anal. 1 (1954) 27-57.
Moore, S., Stein, W.H., Photometric method for use in the chromatography of amino acids, J. Biol. Chem. 176 (1948) 367-388.
Lowry, O.H., Rosebrough, N.J., Fan, A.L., Randall, R.J., Protein measurement with Folin-phenol reagent, J. Biol. Chem. 193 (1955) 265-275.
Rodriguez, R., Fernandez, B., Benzylaminopurine effect on oak, filbert, chestnut and walnut seeds in culture, An. Edafol. Agrobiol. 41 (1982) 11-12.
Zerbe R., Wild A., The effects of gibberellic acid and kinetin on fresh weight, dry weight, leaf area, contents of soluble reducing sugars, soluble proteins, chlorophylls and cytochrome f. Photosynthesis. Vol. VI. Photosynthesis and productivity, in: Akoyunogolu G. (Ed.), Photosynthesis and environment, Proc. V Int. Cong. Photosynthesis, Sept. 7-13, Balaban International Science Services, 1980, Halkidiki, Greece, 1981, pp. 349-351.
Wang, S.Y., Steffens, G.L., Postharvest responses of `Spartan' apples to preharvest paclobutrazol treatment, Hortscience 22 (1987) 276-278.
Steffens, G.L., Wang, S.Y., Steffens, C.L., Brennant, T., Influence of paclobutrazol (PP333)) on apple seedling growth and physiology, Proc. Plant Growth Regul. Soc. Am. 10 (1983) 195-205.
Wang, S.Y., Byun, J.K., Steffens, G.L., Controlling plant growth via the gibberellin biosynthesis system. II. Biochemical and physiological alterations in apple seedlings, Physiol. Plantarum 63 (1985) 169-175. CrossRef
Nagaraju V., Tissue culture studies in Gladiolus, Ph.D. thesis, Gauhati Univ., Guwahati, India, 1994.
Upadhyay, A., Davis, T.D., Sankhla, N., Some biochemical changes associated with paclobutrazol induced adventitious root formation on bean hypocotyl cuttings, Ann. Bot. Fenn. 57 (1986) 309-315.