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Breeding and evaluation of Musa hybrids resistant to Fusarium oxysporum f. sp. cubense race 1

Published online by Cambridge University Press:  23 January 2009

Thukkaram Damodaran ,
Neelakandan Kumar  and
Mathiyazhagan Kavino
Thukkaram Damodaran
Affiliation:
Div. Hortic. For., Cent. Agric. Res. Inst., Port Blair-744 101, Andaman Nicobar Isl., India
Neelakandan Kumar
Affiliation:
Dep. Fruit Crops, Fac. Hortic., Tamil Nadu Agric. Univ., Coimbatore, India
Mathiyazhagan Kavino
Affiliation:
Dep. Fruit Crops, Fac. Hortic., Tamil Nadu Agric. Univ., Coimbatore, India
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Abstract

Introduction. Wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is the most important disease of banana worldwide, causing severe yield losses in commercial and local consumption cultivars. Chemical control is currently the most used method to manage the Fusarium wilt of banana, although it is toxic, expensive and dangerous. Therefore, control through genetic improvement is widely encouraged. Hence, breeding was carried out to develop banana hybrids with putative resistance. Materials and methods. Hybridization of identified resistant diploids with commercial triploids and tetraploids was carried out; it resulted in development of 22 hybrids with improved agronomic characters. In a second step, screening of these hybrids for resistance to Foc race 1 was attempted using the double cup method of challenge inoculation with the pathogen in the roots of the hybrids at optimum level. Host responses of the susceptible and resistant hybrids were examined under greenhouse conditions through biochemical and isozyme analysis. Results. Six hybrids among the 22 hybrids with improved agronomic characters were selected based on their yield and they were evaluated for resistance. Three of them were resistant to Foc and the others were susceptible. The resistance mechanism involving the relation of enzymes such as peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine lyase (PAL), and biochemicals such as phenols, proline and lignin showed relatively higher activity in resistant hybrids and parents than susceptible ones. Isozyme analysis of PO and PPO carried out using selected resistant and susceptible hybrids revealed the induction of specific isoforms in the resistant hybrids upon challenge inoculation. Conclusion. Three hybrids were identified as potential hybrids with good yield and resistance to Foc race 1.

Keywords

IndiaMusaFusarium oxysporumdisease controldisease resistanceplant breedingdefence mechanismsenzymatic analysispolyphenol oxidaseperoxidasesphenylalanine ammonia lyaseIndeMusaFusarium oxysporumcontrôle de maladiesrésistance aux maladiesamélioration des plantesmécanisme de défenseanalyse enzymatiquepolyphénol oxydasepéroxydasephénylalanine ammonia lyase
Type
Research Article
Information
Fruits , Volume 64 , Issue 1 , January 2009 , pp. 3 - 12
Copyright
© CIRAD, EDP Sciences, 2009

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References

Shepherd K., History and methods of banana breeding, InfoMusa 3(1) (1994) 10–11.
Sathiamoorthy S., Balamohan T.N., Improvement of Banana, in: Chadha K.L., Pareek D.P. (Eds.), Advances in Horticulture Vol. 1, Fruit crops part I., Malhotra Publ. House, New Delhi, India, 1993.
Buddenhagen I.W., Banana breeding and Fusarium wilt, in: Fusarium wilt of banana, APS Press, St. Paul, Minnesota, USA, 1990, pp. 107–113.
Thangavelu R., Sundararaju P., Sathiamoorthy S., Reghuchander T., Velazhahan R., Nakkeeran S., Palanisamy A., Status of Fusarium wilt of banana in India, in: Molina A.B., Masdek N.H.N., Liew K.W. (Eds.), Proc. Int. workshop on the banana Fusarium wilt disease, INIBAP, Montpellier, France, 1999, pp. 58–63.
Rowe P.R., Rosales F.E., Bananas and Plantains, in: Janick J., Moore J.N., (Eds.), Advances in fruit breeding, John Wiley & Sons Inc., New York, USA, 1993.
Rowe P.R., Richardson D.L., Breeding bananas for disease resistance, quality and yield, Tech. Bull. No. 2., Trop. Agric. Res. Serv. (SIATSA), La Lima, Honduras, 1975.
Damodaran, T., Kumar, N., Dolezel, J., Suchankova, P., Kavino, M., Hybridization and ploidy determination in new banana hybrids, Phytomorphol. 56 (3–4) (2006) 1–6.
Snyder W.C., Hansen H.W., The species concept in Fusarium, Am. J. Bot. (27) (1940) 64–67.
Brake, V.M., Pegg, K.G., Irwin, J.A.A.G., Chaseling, J., The influence of temperature, inoculum level and race of Fusarium oxysporum f. sp. cubsense on disease reaction of banana cv. Cavendish, Aust. J. Agric. Res. 46 (3) (1995) 673–685. CrossRef
Spies J.R., Colorimetric procedure for amino acid and. phenol, in: Colowick S.P., Kaplan W.O. (Eds.), Methods in enzymology, Acad. Press, New York, USA,1955, pp. 467–468.
Chesson, A., The maceration of line flax under anaerobic condition, J. Appl. Bacteriol. 45 (1978) 219–230. CrossRef
Hammerschmidt, R., Nuckles, E.M., Kuc, J., Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium, Physiol. Plant Pathol. 20 (1982) 73–82. CrossRef
Mayer, A.M., Haul, E., Shaul, R.B., Assay of catechol oxidase, a critical comparison of method, Phytochem. 5 (1965) 783–789. CrossRef
Ross W.W., Sederoff R.R., Phenylalanine ammonia lyase from loblolly pine; Purification of the enzyme and isolation of complementary DNA clones, Plant Physiol. 98 (1992) 380–386. CrossRef
Sindhu, J.S., Ravi, S., Minocha, J.L., Peroxidases isozyme patterns in primary trisomics of pearl millet, Theor. Appl. Genet. 68 (1984) 79–182.
Jayaraman, K.S., Ramanuja, M.N., Vijayaraghavan, P.K., Vaidyanathan, C.S., Oxidative enzyme in pearl millet, Food Chem. 24 (1987) 203. CrossRef
Dodds, K.S., Genetical and cytological studies of Musa. VI. The development of female cells of certain edible diploids, J. Genet. 46 (1945) 161–179. CrossRef
Ortiz, R., Vuylsteke, D., Inheritance of black Sigatoka resistance in plantain and Banana (Musa spp.) hybrids, Theor. Appl. Genet. 89 (1994) 156–162.
Gunavathi J., Studies on resistance to Panama wilt (Fusarium oxysporum f. sp. cubense) in certain banana hybrids, Tamil Nadu Agric. Univ., Thesis, Coimbatore, India, 2000.
Levine, A., Tenhaken, R., Dixon, R., Lamb, C., H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response, Cell 79 (1994) 583–593. CrossRef
Stumm, D., Gessker, C., Role of papillae in induced systemic resistance of cucumber against Colletotrichum lagenarium, Physiol. Mol. Plant Pathol. 29 (1986) 405–410. CrossRef
Gross, G., The biochemistry of lignification, Adv. Bot. Res. 8 (1980) 25–63. CrossRef
Abbattista Gentile, I., Matta, A., Production of some effects of ethylene in relation to Fusarium wilt of tomato, Physiol. Plant Pathol. 5 (1975) 27–35. CrossRef
Matta, A., Diamond, A.E., Symptoms of Fusarium wilt in relation to quantity of fungus and enzyme activity in tomato stems, Phytopathol. 53 (1963) 574–578.
Miyazawa, J., Kawabata, T., Ogasawara, N., Induction of an acidic isozyme of peroxidase and acquired resistance to wilt disease in response to treatment of tomato roots with 2-furoic acid, 4-hydroxybenzoic hydrazide or salicylic hydrazide, Physiol. Mol. Plant Pathol. 58 (1998) 115–125. CrossRef
Lawton, M.A., Dixon, R.A., Hahlbrock, K., Lamb, C.J., Rapid induction of phenylalanine ammonia lyase and of chalcone synthase synthesis in elecitor treated plant cells, Eur. J. Biochem. 129 (1983) 593–601. CrossRef
Cuypers, B., Schmelzer, E., Hahlbrock, K., In-situ localisation of early accumulated phenylalanine ammonia lyase mRNA around penetration site of Phytophthora infestans in potato leaves, Mol. Plant-Microb. Interact. 1 (1988) 157–160. CrossRef
Shivakumar, P.D., Geetha, H.M., Shetty, H.S., Peroxidase activity and isozyme analysis of pearl millet seedlings and their implications in downy mildew disease resistance, Plant Sci. 164 (2003) 85–93. CrossRef
Kavino M., Kumar N., Damodaran T., Harish S., Saravanakumar D., Biochemical markers as a useful tool for the early identification of Fusarium oxysporum f. sp. cubense, race 1 resistance banana clones, Arch. Phytopathol. Plant Prot. (2008) 1–10.
Sariah M., Lim C.L., Tariq S.A., Use of biochemical marker as a measure of resistance towards Fusarium wilt of banana, in: Molina A.B., Masdek N.H.N., Liew K.W. (Eds.), Proc. Int. Workshop on the banana Fusarium wilt disease, INIBAP, Montpellier, France, 1999, 243–249.
Reuveni, R., Shimmoni, M., Crute, I.R., An Association between high peroxidase activity in lettuce (Lactuca sativa) and field resistance to downy mildew (Bremia lactuca), J. Phytopathol. 132 (1992) 312–318. CrossRef
Kavino, M., Harish, S., Kumar, N., Saravanakumar, D., Damodaran, T., Soorianathasundaram, K., Samiappan, R., Rhizosphere and endophytic bacteria for induction of systemic resistance of banana plantlets against bunchy top virus, Soil Biol. Biochem. 39 (2007) 1087–1098. CrossRef
Umesha, S., Shylaja, M.D., Shetty, H.S., Lytic activity in pearl millet: its role in downy mildew disease resistance, Plant Sci. 157 (2000) 33–41. CrossRef