Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2025-01-05T12:04:45.533Z Has data issue: false hasContentIssue false
  • English
  • Français

Restriction Fragment Length Polymorphisms (RFLPS) and Crop Improvement

Published online by Cambridge University Press:  03 October 2008

Nevin Dale Young
Nevin Dale Young
Affiliation:
495 Borlaug Hall, University of Minnesota, Department of Plant Pathology, St. Paul, Minnesota, USA, 55108
Get access Rights & Permissions [Opens in a new window]

Summary

Restriction fragment length polymorphisms (RFLPs) are genetic markers based on cloned molecules of DNA. Using RFLPs, scientists can construct high density genetic linkage maps and locate economically important genes. Once a gene for a valuable trait has been mapped with RFLPs, desirable genotypes can be selected using RFLPs rather than scoring for the trait itself. This is important when a trait is recessive, difficult to score, or obscured by other characters. RFLPs are particularly valuable in mapping genes controlling complex polygenic characters, including those fundamental to crop improvement. The first RFLP maps were developed in crop species primarily important in temperate countries. Now RFLP maps for major tropical and sub-tropical crop species are also being developed.

Type
Research Article
Information
Experimental Agriculture , Volume 28 , Issue 4 , October 1992 , pp. 385 - 398
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Behare, J., Latterot, H., Sarfatti, M. & Zamir, D. (1991). Restriction fragment length polymorphism mapping of the Stemphylium resistance gene in tomato. Molecular Plant-Microbe Interactions 4:489–492.CrossRefGoogle Scholar
Bonierbale, M. W., Plaisted, R. L. & Tansklcy, S. D. (1988). RFLP maps based on common sets of clones reveal modes of chromosome evolution in potato and tomato. Genetics 120:1095–1103.CrossRefGoogle Scholar
Dallas, J. F. (1988). Detection of DNA “fingerprints” of cultivated rice by hybridization with a human minisatellite DNA probe. Proceedings of the National Academy of Science U.S.A. 85:6831–6835.CrossRefGoogle ScholarPubMed
Diers, B. W., Mansur, L., Imsande, J. & Shoemaker, R. C. (1991). Mapping of Phytophthora resistance loci in soyabean with restriction fragment length polymorphism markers. Crop Science. (In press).Google Scholar
Doebly, J., Stec, A., Wendel, J. & Edwards, M. (1990). Genetic and morphological analysis of a maizeteosinte F2 populations: Implications for the origin of maize. Proceedings of the National Academy of Science U.S.A. 87:9888–9892.CrossRefGoogle Scholar
Drabo, I., Redden, R., Smithson, J. B. & Aggarwal, V. D. (1984). Inheritance of seed size in cowpea (Vigna unguiculata (L.) Walp.). Euphytica 33:929–934.CrossRefGoogle Scholar
Fujii, K., Ishimoto, M., Kitamura, K. (1989). Patterns of resistance to bean weevils (Bruchidae) in Vigna radiata-sublobata complex inform the breeding of new resistant varieties. Applied Entomological Zoology 24:126–132.CrossRefGoogle Scholar
Gawel, N. J. & Jarret, R. L. (1991). Chloroplast DNA restriction fragment length polymorphisms (RFLPs) in Musa species. Theoretical and Applied Genetics 81:783–786.CrossRefGoogle ScholarPubMed
Helentjaris, T. (1987). A genetic linkage map for maize based on RFLPs. Trends in Genetics 3:217–221.CrossRefGoogle Scholar
Hinze, K., Thompson, R. D., Ritter, E., Salamini, F. & Schulze-Lefert, P. (1991). Restriction fragment length polymorphism-mediated targeting of the ml-o resistance locus in barley (Hordeum vulgare). Proceedings of the National Academy of Science U.S.A. 88:3691–3695.CrossRefGoogle ScholarPubMed
Hulbert, S. H., Ilott, T. W., Legg, E. J., Lincoln, S. E., Lander, E. S. & Michelmore, R. W. (1988). Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics: 947–958.CrossRefGoogle ScholarPubMed
Hulbert, S. H., Richter, T. E., Axtell, J. D. & Bennetzen, J. L. (1990). Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes. Proceedings of the National Academy of Science U.S.A. 87:4251–4255.CrossRefGoogle ScholarPubMed
Keim, P., Diers, B. W. & Shoemaker, R. C. (1990). Genetic analysis of soybean hard seededness with molecular markers. Theoretical and Applied Genetics 79:465–469.CrossRefGoogle ScholarPubMed
Klein-Lankhorst, R., Rietveld, P., Machiels, B., Verkerk, R., Weide, R., Gebhardt, C., Koornneef, M. & Zabel, P. (1991). RFLP markers linked to the root knot nematode resistance gene Mi in tomato. Theoretical and Applied Genetics 81:661–667.CrossRefGoogle Scholar
Kochert, G., Halward, T., Branch, W. D. & Simpson, C. E. (1991). RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species. Theoretical and Applied Genetics 81:565–570.CrossRefGoogle ScholarPubMed
Lander, E. S. & Botstein, D. (1990) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199.CrossRefGoogle Scholar
Landry, B. S., Kesseli, R. V., Farrara, B. & Michelmore, R. W. (1987). A genetic map of lettuce (Lactuca saliva L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337.CrossRefGoogle Scholar
Lee, M., Godshalk, E. B., Lamkey, K. R. & Woodman, W. W. (1989). Association of restriction fragment length polymorphisms among maize inbreds with agronomic performance of their crosses. Crop Science 29:1067–1071.CrossRefGoogle Scholar
Martin, B., Nienhuis, J., King, G. & Schaefer, A. (1989). Restriction fragment length polymorphisms associated with water use efficiency in tomato. Science 243:1725–1728.CrossRefGoogle ScholarPubMed
Martin, G. B., Williams, J. G. K. & Tanksley, S. D. (1991). Rapid identification of markers linked to a Pseudomonas resistance gene in tomato using random primers and near-isogenic lines. Proceedings of the National Academy of Sciences U.S.A. 88:2336–2340.CrossRefGoogle ScholarPubMed
McCouch, S. R., Kochert, G., Yu, Z. Y., Khush, G. S., Coffman, W. R. & Tanksley, S. D. (1988). Molecular mapping of rice chromosomes. Theoretical and Applied Genetics 76:815–829.CrossRefGoogle ScholarPubMed
McDonald, B. A. & Martinez, J. P. (1990). RFLP variation among Septoria tritici (teleomorph Mycospharella graminicola) isolates collected from a single wheat field. Phytopathology 80:1368–1373.CrossRefGoogle Scholar
McMullen, M. D. & Louie, R. (1989). The linkage of molecular markers to a gene controlling the symptom response in maize to maize dwarf mosaic virus. Molecular Plant-Microbe Interactions 2:309–314.CrossRefGoogle Scholar
Messeguer, R., Ganal, M., de Vicente, M. C., Young, N. D., Bolkan, H. & Tanksley, S. D. (1991). High resolution RFLP map around the root knot nematode resistance gene (Mi) in tomato. Theoretical and Applied Genetics 82:529–536.CrossRefGoogle ScholarPubMed
Miller, J. C. & Tanksley, S. D. (1990). RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theoretical and Applied Genetics 80:437–448.CrossRefGoogle ScholarPubMed
Muehlbauer, G. J., Staswick, P. E., Specht, J. E., Graef, G. L., Shoemaker, R. C. & Keim, P. (1991). RFLP mapping using near-isogenic lines in the soyabean [Glycine max (L.) Merr.]. Theoretical and Applied Genetics 81:189–198.CrossRefGoogle ScholarPubMed
Nienhuis, J., Helentjaris, T., Slocum, M., Ruggero, B. & Schaefer, A. (1987). Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Science 27:797–803.CrossRefGoogle Scholar
Ottaviano, E., Sari Gorla, M. Pè. E., & Frova, C. (1991). Molecular markers (RFLPs and HSPs) for the genetic dissection of thermotolerance in maize. Theoretical and Applied Genetics 81:713–719.CrossRefGoogle ScholarPubMed
Paterson, A., Damon, S., Hewitt, J. D., Zamir, D., Rabinowitch, H. D., Lincoln, S. E., Lander, E. S. & Tanksley, S. D. (1991). Mendelian factors underlying quantitative traits in tomato: Comparison across species, generations, and environments. Genetics 127:181–197.CrossRefGoogle ScholarPubMed
Paterson, A. H., DeVerna, J. W., Lanini, B. & Tanksley, S. D. (1990). Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics 124:735–742.CrossRefGoogle Scholar
Paterson, A. H., Lander, E. S., Hewitt, J. D., Peterson, S., Lincoln, S. E. & Tanksley, S. D. (1988). Resolution of quantitative traits into Mendelian factors using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.Google Scholar
Sarfatti, M., Katan, J., Fluhr, R. & Samir, D. (1989). An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene 12. Theoretical and Applied Genetics 78:755–759.CrossRefGoogle Scholar
Sharp, P. J., Chao, S., Desai, S. & Gale, M. D. (1989). The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homeologous chromosome arm. Theoretical and Applied Genetics 78:342–348.CrossRefGoogle Scholar
Slocum, M. K., Figdore, S. S., Kennard, W. C., Suzuki, J. Y. & Osborn, T. C. (1990). Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theoretical and Applied Genetics 80:57–64.CrossRefGoogle ScholarPubMed
Tanksley, S. D., Miller, J. C., Paterson, A. H. & Bernatzky, R. (1988). Molecular mapping of plant chromosomes. In Chromosome Structure and Function, 157–173 (Ed. by Gustafson, J. F. and Appels, R.). New York: Plenum.CrossRefGoogle Scholar
Tanksley, S. D., Young, N. D., Paterson, A. H. & Bonierbale, M. W. (1989). RFLP mapping in plant breeding: New tools for an old science. Bio/Technology 7:257–264.Google Scholar
Vallejos, C. E. & Chase, C. D. (1991). Linkage between isozyme markers and a locus affecting seed size in Phaseolus vulgaris L. Theoretical and Applied Genetics 81:413–419.CrossRefGoogle Scholar
Young, N. D. (1990). Potential applications of map-based cloning to plant pathology. Physiological and Molecular Plant Pathology 37:81–94.CrossRefGoogle Scholar
Young, N. D. & Tanksley, S. D. (1989). RFLP analysis of the size of chromosomal segments retained around the Tm-2 locus of tomato during backcross breeding. Theoretical and Applied Genetics 77:353–359.CrossRefGoogle ScholarPubMed
Young, N. D., Zamir, D., Ganal, M. W. & Tanksley, S. D. (1988). Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:579–585.CrossRefGoogle Scholar
Yu, Z. H., Mackill, D. J., Bonman, J. M. & Tanksley, S. D. (1991). Tagging genes for blast resistance in rice via linkage to RFLP markers. Theoretical and Applied Genetics 81:471–476.CrossRefGoogle ScholarPubMed