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A Rapid Fluorescent-dye Technique for Measuring Root Length

Published online by Cambridge University Press:  03 October 2008

M. McGowan ,
M. J. Armstrong  and
J. A. Corrie
M. McGowan
Affiliation:
University of Nottingham, School of Agriculture, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, England
M. J. Armstrong
Affiliation:
University of Nottingham, School of Agriculture, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, England
J. A. Corrie
Affiliation:
University of Nottingham, School of Agriculture, Sutton Bonington, Loughborough, Leicestershire, LE12 5RD, England
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Summary

A fluorescent-dye technique has been developed to measure root length of field crops. Roots are washed from soil samples, stained for about 30 min in a solution of fluorescein diacetate in acetone, washed, spread out on a tension table and photographed under UV light. Lengths of fluorescent roots recorded on the photograph are estimated by line intersection. The method is quick, simple, inexpensive and largely eliminates the usual time-consuming, tedious process of hand-sorting live roots from organic debris. Whereas all the roots of young laboratory-grown plants take up the dye, some older roots of field-grown crops do not fluoresce. Data indicate that the method may also give information on root diameter.

Type
Research Article
Information
Experimental Agriculture , Volume 19 , Issue 3 , July 1983 , pp. 209 - 216
Copyright
Copyright © Cambridge University Press 1983

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References

REFERENCES

Arnold, C. R., Rolls, P. J. & Stewart, J. C. J., (1971). Applied Photography, 1st Ed.London: Focal Press.Google Scholar
Clements, C. R. (1966). A simple and reliable tension table. Journal of Soil Science 17:133–136.CrossRefGoogle Scholar
Fisher, C. (1971). The new quantimet 720. The Microscope 1:1–20.Google Scholar
Newman, E. I. (1966) A method for estimating the total length of roots in a sample.journal of applied Ecology 3:139–145.CrossRefGoogle Scholar
Richards, D. F., Gaubran, J. H., Garwoli, W. N. & Doly, M. W. (1979). A method for determining root length. Plant and Soil 52:69–76.CrossRefGoogle Scholar
Rowse, H. R. & Phillips, D. A. (1974). An instrument for estimating the total length of root in a sample. Journal of applied Ecology 11:309–314.CrossRefGoogle Scholar
Voorhees, W. B., Carlson, V. A. & Hallauer, E. A. (1980). Root length measurement with a computer-controlled digital scanning microdensitometer. Agronomy Journal 71:847–851.CrossRefGoogle Scholar
Wildholm, J. M. (1979). The use of fluorescein diacetate and pheno safranine for determining viability of cultured plant cells. Stain Technology 47:189–194.CrossRefGoogle Scholar