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PLANT GROWTH-PROMOTING BACTERIAL ENDOPHYTES FROM SUGARCANE AND THEIR POTENTIAL IN PROMOTING GROWTH OF THE HOST UNDER FIELD CONDITIONS

Published online by Cambridge University Press:  06 November 2012

HEMLATA CHAUHAN ,
D. J. BAGYARAJ  and
ANITA SHARMA
HEMLATA CHAUHAN*
Affiliation:
Department of Microbiology, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145, India
D. J. BAGYARAJ
Affiliation:
Centre for Natural Biological Resources and Community Development, No. 41, RBI Colony, Anand Nagar, Bangalore 560024, India
ANITA SHARMA
Affiliation:
Department of Microbiology, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145, India
*
‡Corresponding author. Email: [email protected]
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Summary

Ten endophytic bacteria were isolated from different sugarcane varieties growing in the Crop Research Centre, Pantnagar on nitrogen-free medium. Plant growth-promoting potential of the isolates was reported in terms of indole acetic acid (IAA) production, phosphorus solubilization, siderophore production and antagonistic action against the pathogen Colletotrichum falcatum, which causes red rot disease in sugarcane in vitro. All the isolates were able to produce IAA (4.8–9 μg ml−1); three isolates (H3, H5 and H14) solubilized insoluble phosphorus on Pikovaskaya's agar; two isolates (H10 and H14) showed siderophore production on Chrome-azurol S (CAS) agar and antagonism against C. falcatum was exhibited by two isolates (H14 and H15) in a dual plate assay. 16 S rRNA sequencing identified isolates H3 and H12 as Pseudomonas spp., and H8, H14 and H15 as Bacillus spp. A field experiment on sugarcane was conducted with five plant growth-promoting bacterial endophytes Pseudomonas spp. (H3 and H12) and Bacillus spp. (H8, H14 and H15) along with standard strains of Gluconacetobacter and Azospirillum spp. Plant height, chlorophyll content, total nitrogen and cane length were significantly higher in almost all inoculated plants compared with the uninoculated control. An increase of 40% in cane yield over the control was obtained after inoculation with isolate H15 (Bacillus spp.). This was statistically on par with the standard endophyte Gluconacetobacter diazotrophicus, which resulted in 42% increased cane yield. Identification of new diazotrophs and their promising results towards improving plant growth in the field suggest their use as inoculants in future.

Type
Research Article
Information
Experimental Agriculture , Volume 49 , Issue 1 , January 2013 , pp. 43 - 52
Copyright
Copyright © Cambridge University Press 2012

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References

REFERENCES

Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiology 24:1–15.CrossRefGoogle ScholarPubMed
Baldani, V. L. D., Baldani, J. I. and Dobereiner, J. (1987). Inoculation of field-grown wheat (Triticum aestivum) with Azospirillum spp. in Brazil. Biology and Fertility of Soils 4:57–60.Google Scholar
Bandara, W. M. M. S, Seneviratane, G. and Kulasooriya, S. A. (2006). Interactions among endophytic bacteria and fungi: effects and potentials. Journal of Biosciences 31:645–650.Google Scholar
Bowen, G. D. and Rovira, A. D. (1999). The rhizosphere and its management to improve plant growth. Advances in Agronomy 66:1–102.Google Scholar
Cavalcante, V. A. and Döbereiner, J. (1988). A new acid-tolerant nitrogen-fixing bacterium associated with sugarcane. Plant and Soil 108:23–31.CrossRefGoogle Scholar
Cochran, G. M. and Cox, W. G. (1959). Experimental Designs. New Delhi, India: Asia Publication House.Google Scholar
Costa, J. M. and Loper, J. E. (1994). Characterization of siderophore production by the biological control agent Enterobacter cloacae. Molecular Plant-Microbe Interactions 7:440–448.CrossRefGoogle Scholar
Döbereiner, J. (1992). History and new perspectives of diazotrophs in association with non-leguminous plants. Symbiosis 13:1–13.Google Scholar
Gordon, S. A. and Weber, R. P. (1951). Colorimeteric estimation of indole acetic acid. Plant Physiology 26:192–195.Google Scholar
Govindarajan, M. J., Kwon, S. W. and Weon, H. Y. (2007). Isolation, molecular characterization and growth-promoting activities of endophytic sugarcane diazotroph Klebsiella sp. GR9. World Journal of Microbiology & Biotechnology 23:997–1006.CrossRefGoogle Scholar
Jackson, M. L. (1973). Soil Chemical Analysis. New Delhi, India: Prentice Hallpp. 186–192, 195–196.Google Scholar
Krotkzy, A. and Werner, D. (1987). Nitrogen fixation in Pseudomonas stutzeri. Archives of Microbiology 147:48–57.Google Scholar
Lee, S., Flores-Encarnacion, M., Contreras-Zentella, M., Garcia- Flores, L., Escamilla, J. E. and Kennedy, C. (2004). Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter diazotrophicus, strains with mutations in cytochrome C biogenesis genes. Journal of Bacteriology 186:5384–5391.Google Scholar
Mahesh Kumar, K. S., Krishnaraj, P. U. and Alagawadi, A. R. (1999). Mineral phosphate solubilizing activity of Acetobacter diazotrophicus: A bacterium associated with sugarcane. Current Science 76:874–875.Google Scholar
Mehnaz, S. (2011). Plant growth promoting bacteria associated with sugarcane. In Bacteria in Agrobiology: Crop Ecosystems, 165–187 (Ed Maheshwari, D. K.). Berlin, Germany: Springer-Verlag.CrossRefGoogle Scholar
Melnick, R. L., Zidack, N. K., Bailey, B. A., Maximova, S. N., Guiltinan, M. and Backman, P. A. (2008). Bacterial endophytes: Bacillus spp. from annual crops as potential biological control agents of black pod rot of cacao. Biological Control 46:46–56.Google Scholar
Mirza, M. S., Mehnaz, S., Normand, P., Prigent-Combaret, C., Moënne-Loccoz, Y., Bally, R. and Malik, K. A. (2006). Molecular characterization and PCR detection of a nitrogen-fixing Pseudomonas strain promoting rice growth. Biology and Fertility of Soils 43:163–170.CrossRefGoogle Scholar
Muthukumarasamy, R., Revathi, G. and Lakshminarasimhan, C. (1999). Influence of N fertilization on the isolation of Gluconacetobacter diazotrophicus and Herbaspirillum spp. from Indian sugarcane varieties. Biology and Fertility of Soils 29:157–164.Google Scholar
Nihorimbere, V., Ongena, M., Smargiassi, M., Thonart, P. (2011). Beneficial effect of the rhizosphere microbial community for plant growth and health. Biotechnology, Agronomy, Society and Environment 15 (2):327–337.Google Scholar
Olivares, F. L., Baldani, V. L. D., Reis, V. M., Baldani, J. I. and Döbereiner, J. (1996). Occurrence of endophytic diazotroph Herbaspirillum spp. in roots, stems and leaves predominantly of gramineae. Biology and Fertility of Soils 21:197–200.CrossRefGoogle Scholar
Oliveira, A. M., Canuto, E. L., Reis, V. M. and Baldani, J. I. (2003). Response of micropropagated sugarcane varieties to inoculation with endophytic diazotrophic bacteria. Brazilian Journal of Microbiology 34:59–61.Google Scholar
Oliveira, A. L. M., Urquiaga, S., Döbereiner, J. and Baldani, J. I. (2002). Effect of inoculating endophytic N2-fixing bacteria on micropropagated sugarcane plants. Plant and Soil 242 (2):205–215.Google Scholar
Olsen, S. R. (1965). Estimation of available phosphorus in soils by extraction with sodium carbonate. Cire US Department of Agriculture, Bulletin No. 939.Google Scholar
Peng, S., Biswas, J. C., Ladha, J. K., Gyaneshwar, P. and Chen, Y. (2002). Influence of rhizobial inoculation on photosynthesis and nitrogen content of field grown rice in the tropics. Agronomy Journal 94:925–929.Google Scholar
Pikovaskaya, R. I. (1948). Mobilization of the phosphorus in soil in connection with the vital activity of some microbial sp. Mikrobiologiya 17:362–370.Google Scholar
Rutge, J. R. (1991). Chlorophyll tester helps pinpoint nitrogen needs. Rice Journal 94:6–10.Google Scholar
Schwyn, B. and Neilands, J. (1987). Universal chemical assays for the detection and determination of siderophore. Annals of Biochemistry 160:47–56.CrossRefGoogle Scholar
Sevilla, M., Burris, R. H., Gunapala, N. and Kennedy, C. (2001). Comparison of benefit to sugarcane plant growth and 15N incorporation following inoculation of sterile plants with Acetobacter diazotropicus wild type and Nif − mutant strains. Molecular Plant-Microbe Interactions 14:358–366.Google Scholar
Sevilla, M., de Oliveira, A., Baldani, J., Kennedy, C. (1998). Contributions of the bacterial endophyte Acetobacter diazotrophicus to sugarcane nutrition: a preliminary study. Symbiosis 25:181–191.Google Scholar
Sorokin, I. D., Kravchenko, I. K., Tourova, T. P., Kolganova, T. V., Boulygina, E. S. and Sorokin, D. Y. (2008). Bacillus alkalidiazotrophicus sp. nov., a diazotrophic, low salt-tolerant alkaliphile isolated from Mongolian soda soil. International Journal of Systematic and Evolutionary Microbiology 58:2459–2464.CrossRefGoogle Scholar
Souza, V. L., Eguiarte, G., Avila, R., Capello, C., Gallardo, J. and Montoya, D. Pin˜ero (1994). Genetic structure of Rhizobium etli biovar phaseoli associated with wild and cultivated bean plants (Phaseolus vulgaris and Phaseolus coccineus) in Morelos, Mexico. Applied and Environmental Microbiology 60:1260–1268.Google Scholar
Tondon, H. L. S. (1998). Methods of Analysis of Soils, Plant, Waters and Fertilizers. New Delhi, India: Fertilizer Development and Consultation Organization.Google Scholar
Wakelin, S., Warren, R., Harvey, P. and Ryder, M. (2004). Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biology and Fertility of Soils 40:36–43.CrossRefGoogle Scholar
Wang, H., Wen, K., Zhao, X., Wang, X., Li, A. and Hong, H. (2009). The inhibitory activity of endophytic Bacillus sp. strain CHM1 against plant pathogenic fungi and its plant growth-promoting effect. Crop Protection 28:634–639.Google Scholar
Will, M. E. and Sylvia, D. M. (1990). Interaction of rhizosphere bacteria, fertilizer, and vesicular-arbuscular mycorrrhizal fungi in sea oats. Applied and Environmental Microbiology 56:2073–2079.Google Scholar
You, C. B., Song, H. X., Wang, J. P., Lin, M. and Hai, W. L. (1991). Association of Alcaligenes faecalis with wetland rice. Plant and Soil 137:81–85.CrossRefGoogle Scholar
You, C. and Zhou, F. (1989). Non-nodular endorhizospheric nitrogen fixation in wetland rice. Canadian Journal of Microbiology 35:403–408.Google Scholar