Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-17T16:12:52.556Z Has data issue: false hasContentIssue false
  • English
  • Français

OXIDATIVE ENZYME RESPONSES OF SIX CITRUS ROOTSTOCKS INFECTED WITH PHOMA TRACHEIPHILA (PETRI) KANTSCHAVELI AND GIKASHVILI

Published online by Cambridge University Press:  24 July 2012

AYDIN UZUN ,
UBEYIT SEDAY ,
ERCAN CANIHOS  and
OSMAN GULSEN
AYDIN UZUN*
Affiliation:
Department of Horticulture, Erciyes University, Kayseri, Turkey
UBEYIT SEDAY
Affiliation:
Alata Horticultural Research Station, Erdemli, Mersin, Turkey
ERCAN CANIHOS
Affiliation:
Plant Protection Research Institute, Adana, Turkey
OSMAN GULSEN
Affiliation:
Department of Horticulture, Erciyes University, Kayseri, Turkey
*
‡Corresponding author. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Citrus trees are often exposed to severe infectious diseases. Mal secco caused by Phoma tracheiphila (Petri) Kantschaveli and Gikashvili is one of the most destructive fungal diseases of lemons (Citrus limon Burm. F.). In the present study, antioxidant enzyme activity in different mal secco-resistant and susceptible citrus rootstocks including Cleopatra mandarin (C. reshni Tan.), sour orange (C. aurantium L.), rough lemon (C. jambhiri Lush.), Volkameriana (C. volkameriana Tan. and Pasq.), Carrizo citrange (Poncirus trifoliata L. Raf. X C. sinensis L. Osbeck) and trifoliate orange (P. trifoliata) was investigated. Possible differences in constitutive levels of these antioxidant enzymes and correlations between enzyme levels and mal secco caused by P. tracheiphila were examined. Among the rootstocks, Cleopatra mandarin was found to be resistant to mal secco, whereas rough lemon, sour orange and trifoliate orange were highly susceptible. Total peroxidase (TPX; EC: 1.11.1.7) activity increased in all infected rootstocks. Ascorbate peroxidase (APX; EC: 1.11.1.11) activity increased in most of the rootstocks and no correlation was found between catalase (CAT; EC: 1.11.1.6) activity and mal secco resistance. This study indicates that overall TPX activity is upregulated and APX activity is up- and down-regulated depending on the type of rootstock in response to P. tracheiphila infection.

Type
Research Article
Information
Experimental Agriculture , Volume 48 , Issue 4 , October 2012 , pp. 563 - 572
Copyright
Copyright © Cambridge University Press 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abedi, T. and Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding 46:27–34.CrossRefGoogle Scholar
Alguacil, M. M., Hernandez, J. A., Caravaca, F., Portillo, B. and Roldan, A. (2003). Antioxidant enzyme activities in shoots from three mycorrhizal shrub species afforested in a degraded semi-arid soil. Physiologia Plantarum 118:562–570.CrossRefGoogle Scholar
Arora, A., Sairam, R. K. and Srivastava, G. C. (2002). Oxidative stress and antioxidative system in plants. Current Science 82:1227–1238.Google Scholar
Baker, C. J. and Orlandi, E. W. (1995). Active oxygen in plant pathogenesis. Annual Review Phytopathology 33:299–321.CrossRefGoogle ScholarPubMed
Ballester, A. R., Lafuente, M. T. and Gonzales-Candelas, L. (2006). Spatial study of antioxidant enzymes, peroxidase and phenylalanine ammonia-lyase in the citrus fruit–Penicillium digitatum interaction. Postharvest Biology and Technology 39:115–124.CrossRefGoogle Scholar
Cakmak, I. and Marschner, H. (1992). Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology 98:1222–1227.CrossRefGoogle ScholarPubMed
Ding, L., Charles, M. T., Carisse, O., Tsao, R., Dube, C. and Khanizadeh, S. (2011). Changes in ascorbate–glutathione pathway enzymes in response to Mycosphaerella fragariae infection in selected strawberry genotypes. Archives of Phytopathology and Plant Protection 44:712–725.CrossRefGoogle Scholar
Ehsani-Moghaddam, B., Charles, M. T., Carisse, O. and Khanizadeh, S. (2006). Superoxide dismutase responses of strawberry cultivars to infection by Mycosphaerella fragariae. Journal of Plant Physiology 163:147–153.CrossRefGoogle ScholarPubMed
Gulsen, O. and Roose, M. L. (2001). Lemons: Diversity and relationships with selected Citrus genotypes as measured with nuclear genome markers. Journal of American Society for Horticultural Science 126:309–317.CrossRefGoogle Scholar
Gulsen, O., Uzun, A., Pala, H., Canihos, E. and Kafa, G. (2007). Development of seedless and ‘mal secco’ tolerant mutant lemons through budwood irradiation. Scientia Horticulturae 112:184–190.CrossRefGoogle Scholar
Gulsen, O., Eickhoff, T., Heng-Moss, T., Shearman, R., Baxendale, F., Sarath, G. and Lee, D. (2010). Characterization of peroxidase changes in resistant and susceptible warm-season turfgrasses challenged by Blissus occiduus. Arthropod-Plant Interactions 4:45–55.CrossRefGoogle Scholar
Heng-Moss, T., Sarath, G., Baxendale, F., Novak, D., Bose, S., Ni, X. and Quisenberry, S. (2004). Characterization of oxidative enzyme changes in buffalograsses challenged by Blissus occiduus. Journal of Economic Entomology 97:1086–1095.CrossRefGoogle ScholarPubMed
Hernandez, J. A., Talavera, J. M., Martinez-Gomez, P., Dicenta, F. and Sevilla, F. (2001). Response of antioxidative enzymes to plum pox virus in two apricot cultivars. Physiologia Plantarum 111:313–321.CrossRefGoogle ScholarPubMed
Hernandez, J. A., Rubio, M., Olmos, E., Ros-Barcelo, A. and Martinez-Gomez, P. (2004). Oxidative stress induced by long-term plum pox virus infection in peach (Prunus persica). Physiologia Plantarum 122:486–495.CrossRefGoogle Scholar
Hildebrand, D. F., Rodriguez, J. G., Brown, G. C., Luu, K. T. and Volden, C. S. (1986). Peroxidative responses of leaves in two soybean genotypes injured by twospotted spider mites (Acari: Tetranychidae). Journal of Economic Entomology 79:1459–1465.CrossRefGoogle Scholar
Kumar, N., Ebel, R. C. and Roberts, P. D. (2011). Superoxide dismutase activity in kumquat leaves infected with Xanthomonas axonopodis pv. citri. Journal of Horticultural Science & Biotechnology 86:62–68.CrossRefGoogle Scholar
Kuzniak, E. and Sklodowska, M. (2005). Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta 222:192–200.CrossRefGoogle ScholarPubMed
Mandal, S., Mitra, A. and Mallick, N. (2008). Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. Lycopersici. Physiological and Molecular Plant Pathology 72:56–61.CrossRefGoogle Scholar
Mates, J. M. and Sanchez-Jiménez, F. (1999). Antioxidant enzymes and their implications in pathophysiologic processes. Frontiers in Bioscience 4:339–345.CrossRefGoogle ScholarPubMed
Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7:405–410.CrossRefGoogle ScholarPubMed
Nakano, Y. and Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22:867–880.Google Scholar
Polidoros, A. N., Mylona, P. V. and Scandalios, J. G. (2001). Transgenic tobacco plants expressing the maize Cat2 gene have altered catalase levels that affect plant–pathogen interactions and resistance to oxidative stress. Transgenic Research 10:555–569.CrossRefGoogle ScholarPubMed
Reverberi, M., Betti, C., Fabbri, A. A., Zjalic, S., Spadoni, S., Mattei, B. and Fanelli, C. (2008). A role for oxidative stress in the Citrus limon /Phoma tracheiphila interaction. Plant Pathology 57:92–102.CrossRefGoogle Scholar
SAS Institute (2002). JMP: Statistical Discovery Software, Version 5.0.1. Cary, NC: SAS Institute, Inc.Google Scholar
Solel, Z. and Spiegel-Roy, P. (1978). Methodology of selection of lemon clones for tolerance to mal secco (P. tracheiphila). Phytoparasitica 6:129–134.CrossRefGoogle Scholar
Torres, R., Valentines, M. C., Usall, J., Vinas, I. and Larrigaudiere, C. (2003). Possible involvement of hydrogen peroxide in the development of resistance mechanisms in ‘Golden Delicious’ apple fruit. Postharvest Biology and Technology 27:235–242.CrossRefGoogle Scholar
Tuzcu, O., Cinar, A., Kaplankiran, M., Erkilic, A. and Yesiloglu, T. (1989). Tolerance of some Citrus species and hybrids to mal secco (Phoma tracheiphila Kantshaveli & Gikashvili). Fruits 44:139–148.Google Scholar
Uzun, A., Yesiloglu, T., Aka-Kacar, Y., Tuzcu, O. and Gulsen, O. (2009a). Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Scientia Horticulturae 121:306–312.CrossRefGoogle Scholar
Uzun, A., Gulsen, O., Kafa, G., Seday, U., Tuzcu, O. and Yesiloglu, T. (2009b). Characterization for yield, fruit quality, and molecular profiles of lemon genotypes tolerant to ‘mal secco’ disease. Scientia Horticulturae 122:556–561.CrossRefGoogle Scholar
Vanacker, H., Foyer, C. H. and Carver, T. L. W. (1998). Changes in apoplastic antioxidants induced by powdery mildew attack in oat genotypes with race non-specific resistance. Planta 208:444–452.CrossRefGoogle Scholar
Wu, Q. S., Zou, Y. Z. and Xia, R. X. (2006a). Effects of water stress and arbuscular mycorrhizal fungi on reactive oxygen metabolism and antioxidant production by citrus (Citrus tangerine) roots. European Journal of Soil Biology 42:166–172.CrossRefGoogle Scholar
Wu, Q. S., Zou, Y. Z., Xia, R. X. and Wang, M. (2006b). Effects of arbuscular mycorrhizal fungi on the growth and antioxidant enzymes of micropropagated citrus. Chinese Journal of Applied & Environmental Biology 12:635–639.Google Scholar
Zhang, S., Lu, S., Xu, X., Korpelainen, H. and Li, C. (2010). Changes in antioxidant enzyme activities and isozyme profiles in leaves of male and female Populus cathayana infected with Melampsora larici-populina. Tree Physiology 30:116–128.CrossRefGoogle ScholarPubMed